Interferon-gamma

Publication Title: 
Journal of Biotechnology

It has been widely reported that CHO cells undergo apoptosis in culture, despite supplementation of nutrients through fed-batch strategies. Improvement of cell viability in culture can effectively improve recombinant protein yield through extension of the culture's production lifespan, especially at high cell densities. Heat shock proteins (HSPs) have been reported to demonstrate anti-apoptotic effects against a wide range of physical and chemical stimuli through their ability to bind and act as antagonists to critical apoptotic molecules.

Author(s): 
Lee, Yih Yean
Wong, Kathy T. K.
Tan, Janice
Toh, Poh Choo
Mao, Yanying
Brusic, Vesna
Yap, Miranda G. S.
Publication Title: 
Aging Cell

In mice, monocytes that exhibit a pro-inflammatory profile enter muscle tissue after muscle injury and are crucial for clearance of necrotic tissue and stimulation of muscle progenitor cell proliferation and differentiation. The aim of this study was to test if pro-inflammatory capacity of classically activated (M1) monocytes relates to muscle mass and strength in humans. This study included 191 male and 195 female subjects (mean age 64.2 years (SD 6.4) and 61.9 ± 6.4, respectively) of the Leiden Longevity Study.

Author(s): 
Beenakker, Karel G. M.
Westendorp, Rudi G. J.
de Craen, Anton J. M.
Slagboom, Pieternella E.
van Heemst, Diana
Maier, Andrea B.
Publication Title: 
Natural Product Research

This study aims to explore and evaluate the effects of Semen Ziziphi Spinosae extracts on the serum levels of interleukin (IL)-6, IFN-?, IL-1?, TNF-?, IL-10 and IL-4 in mice, and the regulative effect of Semen Ziziphi Spinosae on the cytokine system. Using an ELISA assay, the serum levels of IL-6, IFN-?, IL-1?, TNF-?, IL-10 and IL-4 were examined in mice after intraperitoneal injection (i.p.) with Semen Ziziphi Spinosae extracts. The results showed that the levels of IL-6 and IL-1?

Author(s): 
Xie, Junbo
Guo, Li
Pang, Guangchang
Wu, Xin
Zhang, Mingchun
Publication Title: 
The American Journal of Tropical Medicine and Hygiene

Single doses (250, 500, 1,000, or 2,000 units/kg) of an ovine polyclonal-specific Fab fragment directed against tumor necrosis factor-alpha (TNF-alpha) were given to 17 adult patients with severe falciparum malaria immediately before treatment with artesunate in a pilot study to assess safety and optimal dosage with a view to future studies. Clinical and laboratory variables were compared with 11 controls. In the groups given Fab, there was a tendency for a faster resolution of clinical manifestations and reduction of fever but also a tendency towards longer parasite clearance times.

Author(s): 
Looareesuwan, S.
Sjostrom, L.
Krudsood, S.
Wilairatana, P.
Porter, R. S.
Hills, F.
Warrell, D. A.
Publication Title: 
Acta Pharmacologica Sinica

AIM: To study the immunosuppressive activity of SM735 {[3-(12-beta-artemisininoxy)] phenoxyl succinic acid}, a synthetic artemisinin derivative with nonsteroidal anti-inflammatory drug structure, with the aim of finding potential immunosuppressive agents. METHODS: Concanavalin A (ConA), lipopolysaccharide (LPS), and mixed lymphocyte reaction (MLR), were used to induce the proliferation of splenocytes, and [3H]-thymidine incorporation was used to evaluate the proliferation of splenocytes.

Author(s): 
Zhou, Wen-liang
Wu, Jin-ming
Wu, Qing-Li
Wang, Jun-Xia
Zhou, Yu
Zhou, Ru
He, Pei-Lan
Li, Xiao-yu
Yang, Yi-fu
Zhang, Yu
Li, Ying
Zuo, Jian-ping
Publication Title: 
British Journal of Pharmacology

BACKGROUND AND PURPOSE: Artemisinin and its derivatives exhibit potent immunosuppressive activity. The purpose of the current study was to examine the immunosuppressive activity of artemether directly on T lymphocytes and to explore its potential mode of action. EXPERIMENTAL APPROACH: In vitro, T-cell proliferation was measured using [(3)H]-thymidine incorporation assay in cells stimulated with ConA, alloantigen and anti-CD3 antibody. CFSE-labeled cell division and cell cycle distribution were monitored by flow cytometry.

Author(s): 
Wang, J.-X.
Tang, W.
Shi, L.-P.
Wan, J.
Zhou, R.
Ni, J.
Fu, Y.-F.
Yang, Y.-F.
Li, Y.
Zuo, J.-P.
Publication Title: 
Molecular Medicine (Cambridge, Mass.)

Cerebral malaria (CM) is associated with excessive inflammatory responses and endothelial activation. Sphingosine 1-phosphate (S1P) is a signaling sphingolipid implicated in regulating vascular integrity, inflammation and T-cell migration. We hypothesized that altered S1P signaling during malaria contributes to endothelial activation and inflammation, and show that plasma S1P levels were decreased in Ugandan children with CM compared with children with uncomplicated malaria.

Author(s): 
Finney, Constance Am
Hawkes, Cheryl A.
Kain, Dylan C.
Dhabangi, Aggrey
Musoke, Charles
Cserti-Gazdewich, Christine
Oravecz, Tamas
Liles, W. Conrad
Kain, Kevin C.
Publication Title: 
PloS One

Earlier studies in this laboratory have shown the potential of artemisinin-curcumin combination therapy in experimental malaria. In a parasite recrudescence model in mice infected with Plasmodium berghei (ANKA), a single dose of alpha,beta-arteether (ART) with three oral doses of curcumin prevented recrudescence, providing almost 95% protection. The parasites were completely cleared in blood with ART-alone (AE) or ART+curcumin (AC) treatments in the short-term, although the clearance was faster in the latter case involving increased ROS generation.

Author(s): 
Vathsala, Palakkod G.
Dende, Chaitanya
Nagaraj, Viswanathan Arun
Bhattacharya, Debapriya
Das, Gobardhan
Rangarajan, Pundi N.
Padmanaban, Govindarajan
Publication Title: 
Iranian Journal of Allergy, Asthma, and Immunology

Recent studies have indicated the profound anti-tumor activity of artemisinin's compounds, among which; arteether is an oil-soluble derivative of artemisinin with an endoperoxide bridge that can induce apoptosis in tumor cells but not in the normal cells. An experiment was carried out on tumor-bearing Balb/c mice to estimate the effects of Arteether on tumor growth and antitumor immune responses. Briefly, 6mg/kg/day of Arteether and diluents were administered to two groups of mice. Tumor sizes were measured using digital verniercallipers.

Author(s): 
Azimi Mohamadabadi, Maryam
Hassan, Zuhair Muhammad
Zavaran Hosseini, Ahmad
Noori, Shekoofe
Mahdavi, Mehdi
Maroufizadeh, Saman
Maroof, Hamidreza
Publication Title: 
BMC complementary and alternative medicine

BACKGROUND: Maharishi Amrit Kalash (MAK) 5, one of the Ayurvedic food supplements, belongs to a group of substances known as Rasayana. MAK5 and other Rasayanas are believed to enhance the body's resistance to infections and disease, and enhance longevity. In this study, we determined the effects of administration of MAK5, one of the Ayurvedic food supplements on immune functions in young and old mice.

Author(s): 
Inaba, Ryoichi
Mirbod, Seyed Mohammad
Sugiura, Haruo

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