Publication Title: 
American Journal of Physiology. Lung Cellular and Molecular Physiology

Surfactant protein D (SP-D), a member of the collectin superfamily, modulates pulmonary inflammatory responses and innate immunity. Disruption of the SP-D gene in mice induces peribronchiolar inflammation, accumulation of large, foamy macrophages, increased bronchoalveolar lavage (BAL) phospholipid, and pulmonary emphysema. We hypothesized that absence of SP-D aggravates hyperoxia-induced injury. To test this, SP-D-deficient (SP-D-/-) and wild-type (SP-D+/+) mice were exposed to 80% or 21% oxygen. Paradoxically, during 14 days of hyperoxia, SP-D-/- mice had 100% survival vs.

Jain, Deepika
Atochina-Vasserman, Elena
Kadire, Helchem
Tomer, Yaniv
Inch, Adam
Scott, Pamela
Savani, Rashmin C.
Gow, Andrew J.
Beers, Michael F.
Publication Title: 
Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology

BACKGROUND: The effect of ageing on several pathologic features of allergic asthma (pulmonary inflammation, eosinophilia, mucus hypersecretion), and their relationship with airway hyperresponsiveness (AHR) is not well characterized. OBJECTIVE: To evaluate lung inflammation, mucus metaplasia and AHR in relationship with age in murine models of allergic asthma comparing young and older mice. METHODS: Young (6 weeks) and older (6, 12, 18 months) BALB/c mice were sensitized and challenged with ovalbumin (OVA).

Busse, Paula J.
Zhang, Teng Fei
Srivastava, Kamal
Schofield, Brian
Li, Xiu-Min
Publication Title: 
American Journal of Physiology. Lung Cellular and Molecular Physiology

Viral infection is associated with approximately one-half of acute exacerbations of chronic obstructive pulmonary disease (COPD), which in turn, accelerate disease progression. In this study, we infected mice exposed to a combination of elastase and LPS, a constituent of cigarette smoke and a risk factor for development of COPD, with rhinovirus serotype 1B, and examined animals for viral persistence, airway resistance, lung volume, and cytokine responses.

Sajjan, Umadevi
Ganesan, Shyamala
Comstock, Adam T.
Shim, Jee
Wang, Qiong
Nagarkar, Deepti R.
Zhao, Ying
Goldsmith, Adam M.
Sonstein, Joanne
Linn, Marisa J.
Curtis, Jeffrey L.
Hershenson, Marc B.
Publication Title: 
Inflammatory Bowel Diseases

BACKGROUND: Interleukin 13 (IL-13) is upregulated in ulcerative colitis (UC) and increases colon epithelial permeability by inducing apoptosis and expression of the pore-forming tight junction protein claudin-2. IL-13 induces activation of signal transducer and activator of transcription 6 (STAT6). However, the STAT6 phosphorylation status in patients with UC is unknown, as is the effect of STAT6 inhibition on colonic epithelium exposed to IL-13.

Rosen, Michael J.
Frey, Mark R.
Washington, M. Kay
Chaturvedi, Rupesh
Kuhnhein, Lindsay A.
Matta, Poojitha
Revetta, Frank L.
Wilson, Keith T.
Polk, D. Brent
Publication Title: 
The Journal of Nutritional Biochemistry

Eotaxin-3 (CCL-26), a potent chemokine for eosinophil recruitment and contributing significantly to the pathogenesis of asthma, is secreted by lung epithelial cells in response to T helper 2 cytokines including interleukin 13 (IL-13). Here we showed that vitamin E forms, but not their metabolites, differentially inhibited IL-13-stimulated generation of eotaxin-3 in human lung epithelial A549 cells. The relative inhibitory potency was γ-tocotrienol (γ-TE) (IC50 ~15 μM)>γ-tocopherol, δ-tocopherol (IC50 ~25-50 μM)>α-tocopherol.

Wang, Yun
Moreland, Michelle
Wagner, James G.
Ames, Bruce N.
Illek, Beate
Peden, David B.
Jiang, Qing
Publication Title: 
European Journal of Immunology

MicroRNAs (miRNAs) play important roles in the regulation of immune responses. There is evidence that miRNAs also participate in the pathogenesis of multiple sclerosis (MS), but how the miRNAs regulate the pathogenesis of MS is still under investigation. The identification of new members of the miRNA family associated with the pathogenesis of MS could facilitate early diagnosis and treatment. Here, we show that the level of miRNA let-7e is significantly upregulated in EAE, an animal model of MS using miRNA array and quantitative real-time PCR.

Guan, Hongbing
Fan, Daping
Mrelashvili, Davit
Hao, Haiping
Singh, Narendra P.
Singh, Udai P.
Nagarkatti, Prakash S.
Nagarkatti, Mitzi
Publication Title: 
Journal of Leukocyte Biology

DHA is a n-3 LCPUFA in fish oil that generally suppresses T lymphocyte function. However, the effect of fish oil on B cell function remains relatively understudied. Given the important role of B cells in gut immunity and increasing human fish oil supplementation, we sought to determine whether DFO leads to enhanced B cell activation in the SMAD-/- colitis-prone mouse model, similar to that observed with C57BL/6 mice. This study tested the hypothesis that DHA from fish oil is incorporated into the B cell membrane to alter lipid microdomain clustering and enhance B cell function.

Gurzell, Eric A.
Teague, Heather
Harris, Mitchel
Clinthorne, Jonathan
Shaikh, Saame Raza
Fenton, Jenifer I.
Publication Title: 
International Immunopharmacology

BACKGROUND: Cissampelos sympodialis Eichl. (Menispermaceae) is a plant found in Northeastern and Southeast of Brazil and hot water infusion of C. sympodialis root bark is largely used in the indigenous and folk medicine to treat several inflammatory disorders, including asthma. Asthma is a chronic inflammatory allergic disease characterized by airway hyperreactivity (AHR), eosinophil tissue infiltration and lung remodeling. The aim of this study was to evaluate the therapeutic effect of C.

Bezerra-Santos, Claudio R.
Vieira-de-Abreu, Adriana
Vieira, Giciane Carvalho
Filho, Jaime R.
Barbosa-Filho, José Maria
Pires, Ana Lucia
Martins, Marco Aurelio
Souza, Heitor S.
Bandeira-Melo, Christianne
Bozza, Patrícia T.
Piuvezam, Marcia R.
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