K562 Cells

Publication Title: 
Antioxidants & Redox Signaling

Several studies have shown that both oxidative stress and inflammation are linked to the process of hypertension and that the immune system is also involved in this age-related process. More specifically, the oxygen stress related to immune system dysfunction seems to have a key role in senescence, in agreement with the oxidation/ inflammation theory of aging. From a practical point of view, and according to our own research, the immune functions change in a similar fashion in hypertension and aging.

Author(s): 
De la Fuente, M.
Hernanz, A.
Vallejo, M. C.
Publication Title: 
Biological & Pharmaceutical Bulletin

Overcoming MDR (multidrug resistance) phenomena is a crucial aspect of cancer chemotherapy research. Artemisinin and its derivatives have been found to inhibit the proliferation of cancer cells in the microM range. They poorly inhibited the function of P-glycoprotein and did not inhibit the function of MRP1-protein. The concentrations required to inhibit by 50% the function of P-glycoprotein are 110+/-5 microM.

Author(s): 
Reungpatthanaphong, Paiboon
Mankhetkorn, Samlee
Publication Title: 
International Journal of Nanomedicine

The present study evaluated whether the magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe(3)O(4) on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot.

Author(s): 
Wang, Ying
Han, Yuxiang
Yang, Yingying
Yang, Jingci
Guo, Xiaonan
Zhang, Jingnan
Pan, Ling
Xia, Guohua
Chen, Baoan
Publication Title: 
Indian Journal of Experimental Biology

Immunomodulatory activity of an Ayurvedic polyherbal formulation, Immu-21 containing extracts of Ocimum sanctum, Withania somnifera, Emblica officinalis and Tinospora cordifolia was studied on proliferative response of splenic leukocytes to T cell mitogens, concanavalin (Con)-A and phytohemagglutinin (PHA) and B cell mitogen, lipopolysaccharide (LPS) in vitro by [3H]-thymidine uptake assay in mice. The cytotoxic activity of Immu-21 was tested by measuring the splenic leukocyte natural killer (NK) cell activity against K 562 cells.

Author(s): 
Nemmani, Kumar V. S.
Jena, G. B.
Dey, C. S.
Kaul, C. L.
Ramarao, P.
Publication Title: 
Phytotherapy research: PTR

Tiliacora racemosa and Semecarpus anacardium, the two plants frequently used in Ayurvedic medicine for the treatment of cancerous diseases, have been selected to examine their action in four human tumour cell lines: acute myeloblastic leukaemia (HL-60), chronic myelogenic leukaemia (K-562), breast adenocarcinoma (MCF-7) and cervical epithelial carcinoma (HeLa). In cells grown in appropriate media the ethanol extract of T. racemosa root, the total alkaloids isolated from this organ and S. anacardium nut oil prepared according to the Ayurvedic principle were found to have cytotoxic activity.

Author(s): 
Chakraborty, Sutapa
Roy, Madhumita
Taraphdar, Amit K.
Bhattacharya, R. K.
Publication Title: 
Indian Journal of Experimental Biology

Immunostimulatory activity of AC II, a registered ayurvedic preparation prepared at Amala Ayurvedic Research Centre for treating HIV and AIDS is reported. AC II administration could significantly enhance the mitogen-induced proliferation of lymphocytes of spleen cells. It was also found to increase cell-mediated immune responses in normal and tumor-bearing control animals. Oral administration of AC II significantly enhanced Natural Killer cell activity in normal and tumor-bearing animals on the 7th day, which was observed earlier than the tumor-bearing control animals and normal animals.

Author(s): 
Tharakan, Sheeja T.
Kuttan, Girija
Kuttan, Ramadasan
Kesavan, M.
Austin, null
Rajagopalan, K.
Publication Title: 
International Journal of Biological Macromolecules

Asparagus racemosus Linn. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant. In the present study, aqueous extract of A. racemosus (ARC) was fractionated and screened for the polysaccharide fraction (ARP). The characterization was done by enzymatic, Size Exclusion, gas chromatography with flame ionization detector (GC-FID), high pressure anion exchange chromatography (HPAEC) and thin layer chromatographic analyses. Phyto-chemical evaluation confirmed the presence of 26.7% of 2→1 linked fructo-oligosaccharides (FOS).

Author(s): 
Thakur, Mayank
Connellan, Paul
Deseo, Myrna A.
Morris, Carol
Praznik, Werner
Loeppert, Renate
Dixit, V. K.
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

In response to inflammatory stimuli (e.g., endotoxin, proinflammatory cytokines) or oxidative stress, macrophages actively release a ubiquitous nuclear protein, high-mobility group box 1 (HMGB1), to sustain an inflammatory response to infection or injury. In this study, we demonstrated mild heat shock (e.g., 42.5 degrees C, 1 h), or enhanced expression of heat shock protein (Hsp) 72 (by gene transfection) similarly rendered macrophages resistant to oxidative stress-induced HMGB1 cytoplasmic translocation and release.

Author(s): 
Tang, Daolin
Kang, Rui
Xiao, Weimin
Jiang, Lei
Liu, Meidong
Shi, Yongzhong
Wang, Kangkai
Wang, Haichao
Xiao, Xianzhong
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

We previously characterized the CTL response of a melanoma patient who experienced tumor regression following vaccination with an ALVAC virus coding for a MAGE-A3 Ag. Whereas anti-vaccine CTL were rare in the blood and inside metastases of this patient, anti-tumor CTL recognizing other tumor Ags, mainly MAGE-C2, were 100 times more frequent in the blood and considerably enriched in metastases following vaccination.

Author(s): 
Carrasco, Javier
Van Pel, Aline
Neyns, Bart
Lethé, Bernard
Brasseur, Francis
Renkvist, Nicolina
van der Bruggen, Pierre
van Baren, Nicolas
Paulus, Robert
Thielemans, Kris
Boon, Thierry
Godelaine, Danièle
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

We previously characterized the CTL response of a melanoma patient who experienced tumor regression following vaccination with an ALVAC virus coding for a MAGE-A3 Ag. Whereas anti-vaccine CTL were rare in the blood and inside metastases of this patient, anti-tumor CTL recognizing other tumor Ags, mainly MAGE-C2, were 100 times more frequent in the blood and considerably enriched in metastases following vaccination.

Author(s): 
Carrasco, Javier
Van Pel, Aline
Neyns, Bart
Lethé, Bernard
Brasseur, Francis
Renkvist, Nicolina
van der Bruggen, Pierre
van Baren, Nicolas
Paulus, Robert
Thielemans, Kris
Boon, Thierry
Godelaine, Danièle

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