Lactobacillus reuteri

Publication Title: 
Veterinary Immunology and Immunopathology

The goal of this study was to define the impact of colonization of gnotobiotic (Gn) pigs with lactic acid bacteria (LAB) on development of intestinal and systemic B cell responses to human rotavirus (HRV). The LAB-specific and total B cell responses were also assessed. Gn pigs were inoculated with LAB (Lactobacillus acidophilus and L. reuteri) and virulent Wa strain HRV (LAB+HRV+), HRV only (LAB-HRV+), LAB only (LAB+HRV-) or mock (LAB-HRV-).

Author(s): 
Zhang, Wei
Azevedo, Marli S. P.
Gonzalez, Ana M.
Saif, Linda J.
Van Nguyen, Trang
Wen, Ke
Yousef, Ahmed E.
Yuan, Lijuan
Publication Title: 
Veterinary Immunology and Immunopathology

Toll-like receptors (TLR) play an important role in the recognition of microbes by host sentinel cells that leads to the subsequent innate and adaptive immune responses. In this study, we evaluated the patterns of TLR2-, TLR3- and TLR9-expressing antigen presenting cells (APCs) in spleen and blood of gnotobiotic (Gn) pigs after colonization with a mixture of two strains of lactic acid bacteria (LAB), Lactobacillus acidophilus and Lactobacillus reuteri or infection with the virulent human rotavirus (HRV) Wa strain.

Author(s): 
Wen, Ke
Azevedo, Marli S. P.
Gonzalez, Ana
Zhang, Wei
Saif, Linda J.
Li, Guohua
Yousef, Ahmed
Yuan, Lijuan
Publication Title: 
BMC microbiology

BACKGROUND: Commensal-derived probiotic bacteria inhibit enteric pathogens and regulate host immune responses in the gastrointestinal tract, but studies examining specific functions of beneficial microbes in the context of biofilms have been limited in scope. RESULTS: Lactobacillus reuteri formed biofilms that retained functions potentially advantageous to the host including modulation of cytokine output and the production of the antimicrobial agent, reuterin. Immunomodulatory activities of biofilms were demonstrated by the abilities of specific L.

Author(s): 
Jones, Sara E.
Versalovic, James
Publication Title: 
Immunology

To model inflammatory bowel disease, we assessed infection with Helicobacter hepaticus 3B1 (ATCC 51449) and a potential probiotic Lactobacillus reuteri (ATCC PTA-6475) in gnotobiotic B6.129P2-IL-10(tm1Cgn) (IL-10(-/-) ) mice. No typhlocolitis developed in germ-free controls (n=21) or in L. reuteri (n=8) or H. hepaticus (n=18) mono-associated mice for 20 weeks post-infection. As positive controls, three specific pathogen-free IL-10(-/-) mice dosed with H. hepaticus developed severe typhlocolitis within 11 weeks. Because L.

Author(s): 
Whary, Mark T.
Taylor, Nancy S.
Feng, Yan
Ge, Zhongming
Muthupalani, Suresh
Versalovic, James
Fox, James G.
Publication Title: 
PloS One

The genomes of four Lactobacillus reuteri strains isolated from human breast milk and the gastrointestinal tract have been recently sequenced as part of the Human Microbiome Project. Preliminary genome comparisons suggested that these strains belong to two different clades, previously shown to differ with respect to antimicrobial production, biofilm formation, and immunomodulation.

Author(s): 
Saulnier, Delphine M.
Santos, Filipe
Roos, Stefan
Mistretta, Toni-Ann
Spinler, Jennifer K.
Molenaar, Douwe
Teusink, Bas
Versalovic, James
Publication Title: 
Gut Microbes

Although commensal microbes have been shown to modulate host immune responses, many of the bacterial factors that mediate immune regulation remain unidentified. Select strains of human-derived Lactobacillus reuteri synthesize immunomodulins that potently inhibit production of the inflammatory cytokine TNF. In this study, genetic and genomic approaches were used to identify and investigate L. reuteri genes required or human TNF immunomodulatory activity. Analysis of membrane fatty acids from multiple L.

Author(s): 
Jones, Sara E.
Whitehead, Kristi
Saulnier, Delphine
Thomas, Carissa M.
Versalovic, James
Britton, Robert A.
Publication Title: 
Microbial Cell Factories

BACKGROUND: Lactobacillus reuteri harbors the genes responsible for glycerol utilization and vitamin B12 synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this island, resides a gene (lreu_1750) that based on its genomic context has been suggested to encode the regulatory protein PocR and presumably control the expression of the neighboring loci. However, this functional assignment is not fully supported by sequence homology, and hitherto, completely lacks experimental confirmation.

Author(s): 
Santos, Filipe
Spinler, Jennifer K.
Saulnier, Delphine M. A.
Molenaar, Douwe
Teusink, Bas
de Vos, Willem M.
Versalovic, James
Hugenholtz, Jeroen
Publication Title: 
Nucleic Acids Research

The ability to efficiently generate targeted point mutations in the chromosome without the need for antibiotics, or other means of selection, is a powerful strategy for genome engineering. Although oligonucleotide-mediated recombineering (ssDNA recombineering) has been utilized in Escherichia coli for over a decade, the successful adaptation of ssDNA recombineering to gram-positive bacteria has not been reported. Here we describe the development and application of ssDNA recombineering in lactic acid bacteria.

Author(s): 
Van Pijkeren, Jan-Peter
Britton, Robert A.
Publication Title: 
Journal of Pediatric Gastroenterology and Nutrition

OBJECTIVES: Beneficial microbes and probiotics are promising agents for the prevention and treatment of enteric and diarrheal diseases in children; however, little is known about their in vivo mechanisms of action. We used a neonatal mouse model of rotavirus diarrhea to gain insight into how probiotics ameliorate acute gastroenteritis. METHODS: Rotavirus-infected mice were treated with 1 of 2 strains of human-derived Lactobacillus reuteri.

Author(s): 
Preidis, Geoffrey A.
Saulnier, Delphine M.
Blutt, Sarah E.
Mistretta, Toni-Ann
Riehle, Kevin P.
Major, Angela M.
Venable, Susan F.
Barrish, James P.
Finegold, Milton J.
Petrosino, Joseph F.
Guerrant, Richard L.
Conner, Margaret E.
Versalovic, James
Publication Title: 
Beneficial Microbes

Probiotic lactic acid bacteria (LAB) have been shown to alleviate inflammation, enhance the immunogenicity of rotavirus vaccines, or reduce the severity of rotavirus diarrhoea. Although the mechanisms are not clear, the differential Th1/Th2/Th3-driving capacities and modulating effects on cytokine production of different LAB strains may be the key. Our goal was to delineate the influence of combining two probiotic strains of Lactobacillus acidophilus and Lactobacillus reuteri on the development of cytokine responses in neonatal gnotobiotic pigs infected with human rotavirus (HRV).

Author(s): 
Azevedo, M. S. P.
Zhang, W.
Wen, K.
Gonzalez, A. M.
Saif, L. J.
Yousef, A. E.
Yuan, L.

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