Macromolecular Substances

Publication Title: 
Cell

The amplification of ribosomal DNA during development of the somatic macronucleus in Tetrahymena thermophila was analyzed by genetic and molecular biological techniques. We have identified an alternate form of the rDNA, structurally distinguishable from the wild-type by an extra cutting site for Bam HI in its nontranscribed spacer. The altered rDNA was inherited in crosses in a simple Mendelian fashion, consistent with the presence of only one rRNA gene copy per haploid genome in the micronucleus.

Author(s): 
Pan, W. C.
Orias, E.
Flacks, M.
Blackburn, E. H.
Publication Title: 
Genes & Development

The reverse transcriptase telomerase is a ribonucleoprotein complex that adds telomeric repeats to chromosome ends, using a sequence within its endogenous RNA component as a template. Although templating domains of telomerase RNA have been studied in detail, little is known about the roles of the remaining residues, particularly in yeast. We examined the functions of nontemplate telomerase residues in the telomerase RNA of budding yeast Kluyveromyces lactis.

Author(s): 
Roy, J.
Fulton, T. B.
Blackburn, E. H.
Publication Title: 
The Journal of Biological Chemistry
Author(s): 
Davidson, B. E.
Blackburn, E. H.
Dopheide, T. A.
Publication Title: 
International Journal of Biological Macromolecules

Asparagus racemosus Linn. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant. In the present study, aqueous extract of A. racemosus (ARC) was fractionated and screened for the polysaccharide fraction (ARP). The characterization was done by enzymatic, Size Exclusion, gas chromatography with flame ionization detector (GC-FID), high pressure anion exchange chromatography (HPAEC) and thin layer chromatographic analyses. Phyto-chemical evaluation confirmed the presence of 26.7% of 2→1 linked fructo-oligosaccharides (FOS).

Author(s): 
Thakur, Mayank
Connellan, Paul
Deseo, Myrna A.
Morris, Carol
Praznik, Werner
Loeppert, Renate
Dixit, V. K.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Standardized extract from the leaves of the Ginkgo biloba tree, labeled EGb761, has been used in clinical trials for its beneficial effects on brain functions, particularly in connection with age-related dementias and Alzheimer's disease (AD). Substantial experimental evidence indicates that EGb761 protects against neuronal damage from a variety of insults, but its cellular and molecular mechanisms remain unknown.

Author(s): 
Luo, Yuan
Smith, Julie V.
Paramasivam, Vijaykumar
Burdick, Adam
Curry, Kenneth J.
Buford, Justin P.
Khan, Ikhlas
Netzer, William J.
Xu, Huaxi
Butko, Peter
Publication Title: 
The Journal of Biological Chemistry

Macrophages are essential components of innate immunity, and apoptosis of these cells impairs mucosal defense to microbes. Helicobacter pylori is a gastric pathogen that infects half of the world population and causes peptic ulcer disease and gastric cancer. The host inflammatory response fails to eradicate the organism. We have reported that H. pylori induces apoptosis of macrophages by generation of polyamines from ornithine decarboxylase (ODC), which is dependent on c-Myc as a transcriptional enhancer.

Author(s): 
Asim, Mohammad
Chaturvedi, Rupesh
Hoge, Svea
Lewis, Nuruddeen D.
Singh, Kshipra
Barry, Daniel P.
Algood, Holly S.
de Sablet, Thibaut
Gobert, Alain P.
Wilson, Keith T.
Publication Title: 
FASEB journal: official publication of the Federation of American Societies for Experimental Biology

Eye lens senile cataract is a major cause of blindness, affecting the elderly in particular. The etiology of the disorder has been elusive, and attempts to delay the onset of senile cataracts have been unsuccessful. The need for more information is underscored by epidemiologists who estimate that the ability to delay cataract formation in humans by only 10 years would eliminate the need for 50% of the cataract extractions performed annually in the United States. The Emory mouse provides the best model for human senile cataracts.

Author(s): 
Taylor, A.
Zuliani, A. M.
Hopkins, R. E.
Dallal, G. E.
Treglia, P.
Kuck, J. F.
Kuck, K.
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