Membrane Potential, Mitochondrial

Publication Title: 
Free Radical Research

Previous studies have shown that glucose-6-phosphate dehydrogenase (G6PD)-deficient cells are under increased oxidative stress and undergo premature cellular senescence. The present study demonstrates that G6PD-deficient cells cultured under 3% oxygen concentration had an extended replicative lifespan, as compared with those cultured under atmospheric oxygen level. This was accompanied by a reduction in the number of senescence-associated beta-galactosidase (SA-beta-Gal) positive and morphologically senile cells at comparable population doubling levels (PDL).

Author(s): 
Ho, Hung-Yao
Cheng, Mei-Ling
Cheng, Pei-Fen
Chiu, Daniel Tsun-Yee
Publication Title: 
Antioxidants & Redox Signaling

SIGNIFICANCE: Among the most highly investigated theories of aging is the mitochondrial theory of aging. The basis of this theory includes a central role for altered or compromised mitochondrial function in the pathophysiologic declines associated with aging. In general, studies in various organisms, including nematodes, rodents, and humans, have largely upheld that aging is associated with mitochondrial dysfunction.

Author(s): 
Pulliam, Daniel A.
Bhattacharya, Arunabh
Van Remmen, Holly
Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: Eucommia ulmoides Oliv. Bark. (EUE), has commonly been used to fortify the muscles and lungs, lower blood pressure, prevent miscarriage, improve the tone of liver and kidneys, and promote longevity the traditional tonic medicines of Korea, China, and Japan. AIM OF THE STUDY: In this study, we investigated that the neuroprotective activities and possible mechanisms of EUE aqueous extract in hydrogen peroxide (H(2)O(2))-induced neuronal cell death in human SH-SY5Y neuroblastoma cells.

Author(s): 
Kwon, Seung-Hwan
Kim, Min-Jung
Ma, Shi-Xun
You, In-Jee
Hwang, Ji-Young
Oh, Ji-Hwan
Kim, Sun-Yeou
Kim, Hyoung-Chun
Lee, Seok-Yong
Jang, Choon-Gon
Publication Title: 
Antioxidants & Redox Signaling

SIGNIFICANCE: Among the most highly investigated theories of aging is the mitochondrial theory of aging. The basis of this theory includes a central role for altered or compromised mitochondrial function in the pathophysiologic declines associated with aging. In general, studies in various organisms, including nematodes, rodents, and humans, have largely upheld that aging is associated with mitochondrial dysfunction.

Author(s): 
Pulliam, Daniel A.
Bhattacharya, Arunabh
Van Remmen, Holly
Publication Title: 
ACS chemical neuroscience

Alzheimer's disease is associated with metabolic deficits and reduced mitochondrial function, with the latter due to the effects of oligomeric amyloid beta peptide (A?O) on the respiratory chain. Recent evidence has demonstrated reduction of epigenetic markers, such as DNA methylation, in Alzheimer's disease. Here we demonstrate a link between metabolic and epigenetic deficits via reduction of mitochondrial function which alters the expression of mediators of epigenetic modifications.

Author(s): 
Mastroeni, Diego
Khdour, Omar M.
Arce, Pablo M.
Hecht, Sidney M.
Coleman, Paul D.
Publication Title: 
Eukaryotic Cell

Intracellular calcium controls several crucial cellular events in apicomplexan parasites, including protein secretion, motility, and invasion into and egress from host cells. The plant compound thapsigargin inhibits the sarcoplasmic-endoplasmic reticulum calcium ATPase (SERCA), resulting in elevated calcium and induction of protein secretion in Toxoplasma gondii. Artemisinins are natural products that show potent and selective activity against parasites, making them useful for the treatment of malaria.

Author(s): 
Nagamune, Kisaburo
Beatty, Wandy L.
Sibley, L. David
Publication Title: 
Journal of Cellular and Molecular Medicine

The present study was designed to determine the effects of artemisinin (ARS) and its derivatives on human ovarian cancer cells, to evaluate their potential as novel chemotherapeutic agents used alone or in combination with a conventional cancer chemotherapeutic agent, and to investigate their underlying mechanisms of action. Human ovarian cancer cells (A2780 and OVCAR-3), and immortalized non-tumourigenic human ovarian surface epithelial cells (IOSE144), were exposed to four ARS compounds for cytotoxicity testing.

Author(s): 
Chen, Tao
Li, Mian
Zhang, Ruiwen
Wang, Hui
Publication Title: 
Journal of Biomedical Science

BACKGROUND: Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial drug with low toxicity. DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although the molecular mechanisms are not well understood. METHODS: In this study, cell counting kit (CCK-8) assay was employed to evaluate the survival of DHA-treated ASTC-a-1 cells.

Author(s): 
Lu, Ying-Ying
Chen, Tong-Sheng
Qu, Jun-Le
Pan, Wen-Liang
Sun, Lei
Wei, Xun-Bin
Publication Title: 
Pharmacological reports: PR

BACKGROUND: Dihydroartemisinin (DHA) exhibits potent anti-malarial and anti-cancer activities. This study aimed to investigate the anti-proliferative effects of a combination of DHA and doxorubicin (DOX) on human breast cancer cells. METHODS: MTT assay and the combination index (CI) were used to show the anti-proliferative effects and calculate the synergism potential, respectively. Flow cytometry assay was used to detect apoptosis and the intracellular accumulation of DOX. JC-1 staining was used to determine the mitochondrial membrane potential.

Author(s): 
Wu, Guo-Sheng
Lu, Jin-Jian
Guo, Jia-Jie
Huang, Ming-Qing
Gan, Li
Chen, Xiu-Ping
Wang, Yi-Tao
Publication Title: 
Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association

Cosmetic, pharmaceutical, food and confectionary industries make increasing use of plant extracts in their products. Despite the widespread use of products containing plant extracts, the mechanisms of their effects are not fully characterized. Bergamot essential oil (BEO; Citrus bergamia, Risso) is a well-known plant extract used in aromatherapy and it has analgesic, anxiolytic and neuroprotective effects in rodents. To elicit neuroprotection, BEO recruits Akt prosurvival pathways. However, Akt stimulates cell proliferation, which may also pose risks for health in case of prolonged use.

Author(s): 
Berliocchi, Laura
Ciociaro, Antonella
Russo, Rossella
Cassiano, Maria Gilda Valentina
Blandini, Fabio
Rotiroti, Domenicantonio
Morrone, Luigi Antonio
Corasaniti, Maria Tiziana

Pages

Subscribe to RSS - Membrane Potential, Mitochondrial