Metabolome

Publication Title: 
PloS One

The aging phenotype in humans has been thoroughly studied but a detailed metabolic profiling capable of shading light on the underpinning biological processes of longevity is still missing. Here using a combined metabonomics approach compromising holistic (1)H-NMR profiling and targeted MS approaches, we report for the first time the metabolic phenotype of longevity in a well characterized human aging cohort compromising mostly female centenarians, elderly, and young individuals.

Author(s): 
Collino, Sebastiano
Montoliu, Ivan
Martin, FranÁois-Pierre J.
Scherer, Max
Mari, Daniela
Salvioli, Stefano
Bucci, Laura
Ostan, Rita
Monti, Daniela
Biagi, Elena
Brigidi, Patrizia
Franceschi, Claudio
Rezzi, Serge
Publication Title: 
Gerontology

The fungicide rapamycin increases lifespan in eukaryotes by interfering with the activity of a serine/threonine kinase called TOR (target of rapamycin). TOR complex 1 (TORC1) is an essential integrator of cellular nutrient cues, growth signals and cellular metabolism. Here, we review major components of TORC1, its downstream effectors and lifespan studies in various organisms involving these signaling components. In particular, we focus on the role of rapamycin in mitochondrial biogenesis, in metabolic regulation and in the control of reactive oxygen species production.

Author(s): 
Pan, Yong
Nishida, Yuya
Wang, Margaret
Verdin, Eric
Publication Title: 
PloS One

The aging phenotype in humans has been thoroughly studied but a detailed metabolic profiling capable of shading light on the underpinning biological processes of longevity is still missing. Here using a combined metabonomics approach compromising holistic (1)H-NMR profiling and targeted MS approaches, we report for the first time the metabolic phenotype of longevity in a well characterized human aging cohort compromising mostly female centenarians, elderly, and young individuals.

Author(s): 
Collino, Sebastiano
Montoliu, Ivan
Martin, FranÁois-Pierre J.
Scherer, Max
Mari, Daniela
Salvioli, Stefano
Bucci, Laura
Ostan, Rita
Monti, Daniela
Biagi, Elena
Brigidi, Patrizia
Franceschi, Claudio
Rezzi, Serge
Publication Title: 
Biological Psychiatry

Peripheral samples, such as blood and skin, have been used for decades in psychiatric research as surrogates for central nervous system samples. Although the validity of the data obtained from peripheral samples has been questioned and other state-of-the-art techniques, such as human brain imaging, genomics, and induced pluripotent stem cells, seem to reduce the value of peripheral cells, accumulating evidence has suggested that revisiting peripheral samples is worthwhile.

Author(s): 
Hayashi-Takagi, Akiko
Vawter, Marquis P.
Iwamoto, Kazuya
Publication Title: 
Malaria Journal

BACKGROUND: Dihydroartemisinin (DHA), a powerful anti-malarial drug, has been used as monotherapy and artemisinin-based combination therapy (ACT) for more than decades. So far, however, the tissue distribution and metabolic profile of DHA data are not available from animal and humans. METHODS: Pharmacokinetics, tissue distribution, mass balance, and elimination of [14C] DHA have been studieded in rats following a single intravenous administration. Protein binding was performed with rat and human plasma.

Author(s): 
Xie, Lisa H.
Li, Qigui
Zhang, Jing
Weina, Peter J.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

The human malaria parasite Plasmodium falciparum is auxotrophic for most amino acids. Its amino acid needs are met largely through the degradation of host erythrocyte hemoglobin; however the parasite must acquire isoleucine exogenously, because this amino acid is not present in adult human hemoglobin. We report that when isoleucine is withdrawn from the culture medium of intraerythrocytic P. falciparum, the parasite slows its metabolism and progresses through its developmental cycle at a reduced rate.

Author(s): 
Babbitt, Shalon E.
Altenhofen, Lindsey
Cobbold, Simon A.
Istvan, Eva S.
Fennell, Clare
Doerig, Christian
Llinás, Manuel
Goldberg, Daniel E.
Publication Title: 
Planta Medica

The genus Echinacea is used as an herbal medicine to treat a variety of ailments. To better understand its potential chemical variation, 40 Echinacea accessions encompassing broad geographical and morphological diversity were evaluated under controlled conditions. Metabolites of roots from these accessions were analyzed by HPLC-photo diode array (HPLC-PDA), GC-MS, and multivariate statistical methods. In total, 43 lipophilic metabolites, including 24 unknown compounds, were detected.

Author(s): 
Wu, Lankun
Dixon, Philip M.
Nikolau, Basil J.
Kraus, George A.
Widrlechner, Mark P.
Wurtele, Eve Syrkin
Publication Title: 
Journal of Proteome Research

Our understanding of the mechanisms by which nonalcoholic fatty liver disease (NAFLD) progresses from simple steatosis to steatohepatitis (NASH) is still very limited. Despite the growing number of studies linking the disease with altered serum metabolite levels, an obstacle to the development of metabolome-based NAFLD predictors has been the lack of large cohort data from biopsy-proven patients matched for key metabolic features such as obesity.

Author(s): 
Barr, J.
Caballería, J.
Martínez-Arranz, I.
Domínguez-Díez, A.
Alonso, C.
Muntané, J.
Pérez-Cormenzana, M.
García-Monzón, C.
Mayo, R.
Martín-Duce, A.
Romero-Gómez, M.
Lo Iacono, O.
Tordjman, J.
Andrade, R. J.
Pérez-Carreras, M.
Le Marchand-Brustel, Y.
Tran, A.
Fernández-Escalante, C.
Arévalo, E.
García-Unzueta, M.
Clement, K.
Crespo, J.
Gual, P.
Gómez-Fleitas, M.
Martínez-Chantar, M. L.
Castro, A.
Lu, S. C.
Vázquez-Chantada, M.
Mato, J. M.
Publication Title: 
American Journal of Respiratory Cell and Molecular Biology

The genetic basis for the underlying individual susceptibility to chlorine-induced acute lung injury is unknown. To uncover the genetic basis and pathophysiological processes that could provide additional homeostatic capacities during lung injury, 40 inbred murine strains were exposed to chlorine, and haplotype association mapping was performed. The identified single-nucleotide polymorphism (SNP) associations were evaluated through transcriptomic and metabolomic profiling.

Author(s): 
Leikauf, George D.
Pope-Varsalona, Hannah
Concel, Vincent J.
Liu, Pengyuan
Bein, Kiflai
Berndt, Annerose
Martin, Timothy M.
Ganguly, Koustav
Jang, An Soo
Brant, Kelly A.
Dopico, Richard A.
Upadhyay, Swapna
Di, Y. P. Peter
Li, Qian
Hu, Zhen
Vuga, Louis J.
Medvedovic, Mario
Kaminski, Naftali
You, Ming
Alexander, Danny C.
McDunn, Jonathan E.
Prows, Daniel R.
Knoell, Daren L.
Fabisiak, James P.
Publication Title: 
Fitoterapia

The majority of bioactive principles in a complex matrix such as natural products and botanical medicines are secondary rather than primary metabolites. In addition to being chemically diverse, the bioactivity of an ethnobotanical can comprise from one to several bioactive compounds, present in a complex mixture. Conventional discovery efforts utilize bioassay-guided fractionation (BGF) to isolate individual active compounds. When applied to complex natural products, BGF is often challenged by an apparent loss of activity during fractionation, resulting in weakly active isolated compounds.

Author(s): 
Inui, Taichi
Wang, Yuehong
Pro, Samuel M.
Franzblau, Scott G.
Pauli, Guido F.

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