Mice, SCID

Publication Title: 
Blood

Although the overproduction of immunoglobulins by short-lived plasma cells accompanying an immune response links with their apoptosis, how long-lived plasma cells adapt to ensure their longevity in this context is obscure. Here, we show that apoptosis signal-regulating kinase 1 (ASK1) contributes to apoptosis of plasma cells because ASK1 activity was induced during differentiation of short-lived plasma cells, and, when produced by ASK1-deficient mice, these cells survived better than those of control mice.

Author(s): 
Lin, Fan-Ru
Huang, Shang-Yi
Hung, Kuo-Hsuan
Su, Shin-Tang
Chung, Cheng-Han
Matsuzawa, Atsushi
Hsiao, Michael
Ichijo, Hidenori
Lin, Kuo-I.
Publication Title: 
PloS One

There is growing evidence and a consensus in the field that most pediatric brain tumors originate from stem cells, of which radial glial cells constitute a subtype. Here we show that orthotopic transplantation of human radial glial (RG) cells to the subventricular zone of the 3rd ventricle--but not to other transplantation sites--of the brain in immunocompromised NOD-SCID mice, gives rise to tumors that have the hallmarks of CNS primitive neuroectodermal tumors (PNETs). The resulting mouse model strikingly recapitulates the phenotype of PNETs.

Author(s): 
Malchenko, Sergey
Sredni, Simone Treiger
Hashimoto, Hitoshi
Kasai, Atsushi
Nagayasu, Kazuki
Xie, Jianping
Margaryan, Naira V.
Seiriki, Kaoru
Lulla, Rishi R.
Seftor, Richard E. B.
Pachman, Lauren M.
Meltzer, Herbert Y.
Hendrix, Mary J. C.
Soares, Marcelo B.
Publication Title: 
Stem Cells (Dayton, Ohio)

Human embryonic stem cells (hESCs) have been reported to exert cytoprotective activity in the area of tissue injury. However, hypoxia/oxidative stress prevailing in the area of injury could activate p53, leading to death and differentiation of hESCs. Here we report that when exposed to hypoxia/oxidative stress, a small fraction of hESCs, namely the SSEA3+/ABCG2+ fraction undergoes a transient state of reprogramming to a low p53 and high hypoxia inducible factor (HIF)-2? state of transcriptional activity.

Author(s): 
Das, Bikul
Bayat-Mokhtari, Reza
Tsui, Micky
Lotfi, Shamim
Tsuchida, Rika
Felsher, Dean W.
Yeger, Herman
Publication Title: 
Antimicrobial Agents and Chemotherapy

Murine models of Plasmodium falciparum malaria may become crucial tools in drug discovery. Here we show that non-myelodepleted NOD-scid IL2Rgamma(null) mice engrafted with human erythrocytes support an infectious burden up to tenfold higher than that supported by engrafted NOD-scid beta2microglobulin(null) mice. The new model was validated for drug discovery and was used to assess the therapeutic efficacy of 4-pyridones, selective inhibitors of P. falciparum cytochrome bc1.

Author(s): 
Jiménez-Díaz, María Belén
Mulet, Teresa
Viera, Sara
Gómez, Vanessa
Garuti, Helen
Ibáñez, Javier
Alvarez-Doval, Angela
Shultz, Leonard D.
Martínez, Antonio
Gargallo-Viola, Domingo
Angulo-Barturen, Iñigo
Publication Title: 
Clinical Cancer Research: An Official Journal of the American Association for Cancer Research

Over-consumption of dietary fat has been suggested to promote the development and progression of prostate cancer in men. The present study was conducted to answer the following questions: (a) Can dietary fat reduction decrease tumor growth rates of Los Angeles prostate cancer (LAPC)-4 xenografts in severe combined immunodeficient (SCID) mice independent of total caloric intake? and (b) Is the insulin-like growth factor (IGF) axis involved in the effects of dietary fat on LAPC-4 tumor growth in SCID mice?

Author(s): 
Ngo, Tung H.
Barnard, R. James
Cohen, Pinchas
Freedland, Stephen
Tran, Chris
deGregorio, Frank
Elshimali, Yahya I.
Heber, David
Aronson, William J.
Publication Title: 
International Journal of Cancer. Journal International Du Cancer

Breast cancer is significantly less prevalent among Asian women, whose diets contain high intake of soy products and tea. The objective of our present study was to identify the combined effects of dietary soy phytochemicals and tea components on breast tumor progression in a clinically relevant in vivo model of MCF-7 androgen-dependent human breast tumor in female SCID mice.

Author(s): 
Zhou, Jin-Rong
Yu, Lunyin
Mai, Zhiming
Blackburn, George L.
Publication Title: 
Clinical Cancer Research: An Official Journal of the American Association for Cancer Research

PURPOSE: To determine whether altering the dietary content of omega-6 (n-6) and omega-3 (n-3) polyunsaturated fatty acids affects the growth of androgen-sensitive prostate cancer xenografts, tumor membrane fatty acid composition, and tumor cyclooxygenase-2 and prostaglandin E(2) (PGE(2)) levels. EXPERIMENTAL DESIGN: Individually caged male severe combined immunodeficiency mice were fed isocaloric 20% kcal fat diets with the fat derived either primarily from n-6 fatty acids (n-6 group) or with the fat consisting of n-6 and n-3 fatty acids in a ratio of 1:1 (n-3 group), and injected s.c.

Author(s): 
Kobayashi, Naoko
Barnard, R. James
Henning, Susanne M.
Elashoff, David
Reddy, Srinivasa T.
Cohen, Pinchas
Leung, Pak
Hong-Gonzalez, Jenny
Freedland, Stephen J.
Said, Jonathan
Gui, Dorina
Seeram, Navindra P.
Popoviciu, Laura M.
Bagga, Dilprit
Heber, David
Glaspy, John A.
Aronson, William J.
Publication Title: 
Carcinogenesis

The objective of this work was to determine the interactive effects between soy bioactive components and tamoxifen (TAM) on prevention of estrogen-dependent breast cancer (BRCA). We initially investigated the effects of soy isoflavone genistein and TAM on the growth and cell cycle progression of estrogen-dependent MCF-7 human BRCA cells, and on the expression of ERalpha, pS2 and EGFR genes in vitro. Genistein or TAM alone inhibited the growth of MCF-7 cells in part via G(1) phase arrest, but their combinations showed suggestive antagonistic effects.

Author(s): 
Mai, Zhiming
Blackburn, George L.
Zhou, Jin-Rong
Publication Title: 
Hepatology (Baltimore, Md.)

Methionine adenosyltransferase (MAT) is an essential enzyme that catalyzes the biosynthesis of S-adenosylmethionine. Hepatic MAT activity falls in chronic liver diseases, and mice lacking Mat1a are predisposed to liver injury and develop hepatocellular carcinoma (HCC) spontaneously by 18 months. The current work examined the hypothesis that liver cancer stem cells contribute to HCC in this model. Livers from 6- and 18-month-old Mat1a-knockout (KO) mice and their wild-type (WT) littermates were fractionated and isolated by flow cytometry.

Author(s): 
Rountree, C. Bart
Senadheera, Shantha
Mato, José M.
Crooks, Gay M.
Lu, Shelly C.
Publication Title: 
Journal of Periodontal Research

BACKGROUND AND OBJECTIVE: Chronic inflammatory bowel disease (IBD) demonstrates some similarities to the dysregulated chronic immunoinflammatory lesion of periodontitis. Trinitrobenzene sulphonic acid (TNBS) and dextran sodium sulphate (DSS) administered to rodents have been shown to elicit inflammatory responses that undermine the integrity of the gut epithelium in a similar manner to IBD in humans. The objective of this study was to evaluate the ability of these chemicals to elicit periodontal inflammation as a novel model for alveolar bone loss.

Author(s): 
Oz, H. S.
Ebersole, J. L.

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