Microscopy, Electron

Publication Title: 
PloS One

BACKGROUND: Recent studies have demonstrated that activation of autophagy increases the lifespan of organisms from yeast to flies. In contrast to the lifespan extension effect in lower organisms, it has been reported that overexpression of unc-51-like kinase 3 (ULK3), the mammalian homolog of autophagy-specific gene 1 (ATG1), induces premature senescence in human fibroblasts. Therefore, we assessed whether the activation of autophagy would genuinely induce premature senescence in human cells.

Author(s): 
Kang, Hyun Tae
Lee, Ki Baek
Kim, Sung Young
Choi, Hae Ri
Park, Sang Chul
Publication Title: 
Annals of the New York Academy of Sciences

Tibetan medicine integrates diet, lifestyle, herbs, and accessory therapies to increase health and longevity. A comparison of the three humor theory of Tibetan medicine and the three thermodynamic phase properties of myelin lipids exemplifies how integrating medical systems can increase understanding of complex chronic disabling conditions.

Author(s): 
Husted, Cynthia
Dhondup, Lobsang
Publication Title: 
Cell

Macronuclear DNA from the protozoan G. chattoni, a holotrichous ciliate, was analyzed. Most, if not all, of the macronuclear DNA is subchromosomal, ranging in size from above 100 kb down to 2.1 kb, with molecules in the lower molecular weight range being resolvable by gel electrophoresis into reproducible, specific, discrete size classes. A prominent class of linear 9.3 kb molecules consists of single free rRNA genes. Upon denaturation of total macronuclear DNA was found as single-stranded circles.

Author(s): 
Katzen, A. L.
Cann, G. M.
Blackburn, E. H.
Publication Title: 
Cell

Macronuclear DNA from the protozoan G. chattoni, a holotrichous ciliate, was analyzed. Most, if not all, of the macronuclear DNA is subchromosomal, ranging in size from above 100 kb down to 2.1 kb, with molecules in the lower molecular weight range being resolvable by gel electrophoresis into reproducible, specific, discrete size classes. A prominent class of linear 9.3 kb molecules consists of single free rRNA genes. Upon denaturation of total macronuclear DNA was found as single-stranded circles.

Author(s): 
Katzen, A. L.
Cann, G. M.
Blackburn, E. H.
Publication Title: 
Journal of Anatomy

Radially oriented ensembles of neurons and their projections, termed minicolumns, are hypothesized to be the basic microcircuit of mammalian cerebral cortex. Minicolumns can be divided into a core and a peripheral neuropil space compartment. The core of minicolumns is constrained by the migratory path of pyramidal cells and their attendant radially oriented projections. Variation in minicolumnar morphometry and density is observed both within and across species.

Author(s): 
Casanova, Manuel F.
Trippe, Juan
Tillquist, Christopher
Switala, Andrew E.
Publication Title: 
Biochemical Pharmacology
Author(s): 
Azarnoff, D. L.
Grady, H. J.
Svoboda, D. J.
Publication Title: 
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica

Ultrastructural changes in Plasmodium gallinaceum oocysts and sporozoites were studies after 5 antimalarials (pyrimethamine, primaquine, artemisinine, 5-p-fluorobenzoxyl-primaquine citrate and nitroquine) were administered to Aedes albopictus. Obvious disfigurement, such as abnormal vacuoles of various sizes in the cytoplasma, thickened oocyst capsules and damaged sporozoite pellical membranes were found in many oocysts and sporozoites in the mosquitoes.

Author(s): 
Hu, C. R.
Chen, P. H.
Chen, J. M.
Huang, Q.
Publication Title: 
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica

The effects of alpha-dimethylamino-cyclohexoxyl-dimethyl gallium (DCDG), a new antimalarial drug developed in China, on the ultrastructure of murine malaria parasites in vivo was studied in comparison with those of chloroquine (CQ) and artemisinin (Art). All these 3 antimalarials were administered ig to mice at dosages of 1-3, 40-80, and 200-400 mg.kg-1 for DCDG, CQ, and Art respectively, based on a similar intensity of morphological changes in the parasites. Blood samples were collected for electron microscopy from 15 min to 48 h after medication.

Author(s): 
Yan, G. H.
Wang, G. J.
Li, Y. C.
Publication Title: 
Acta Pharmacologica Sinica

AIM: The fine structural changes of Plasmodium berghei ANKA strain after treatment with the dihydroartemisinin (DATM) were observed. METHODS: DATM 180 mg.kg-1.d-1 was given ig to outbreed NIH mice infected with P. berghei ANKA strain. Blood samples were collected, embedded and examined by electron microscopy. RESULTS: In P. berghei ANKA strain, 1 h after drug administration, the parasites food vacuole membranes were destroyed, and the pigment grains showed some changes. The nucleus membrane, cytomembrane, and food vacuole membranes were stratified 2 h after administration.

Author(s): 
Chen, P. Q.
Yuan, J.
Du, Q. Y.
Chen, L.
Li, G. Q.
Huang, Z. Y.
Yang, D. D.
Wu, L. N.
Publication Title: 
Memórias Do Instituto Oswaldo Cruz

Progress has been made over the last decade with the development and clinical use of artemether as an agent against major human schistosome parasites. The tegument has been identified as a key target of artemether, implying detailed studies on ultrastructural damage induced by this compound. We performed a temporal examination, employing a transmission electron microscope to assess the pattern and extent of ultrastructural alterations in adult Schistosoma mansoni harboured in mice treated with a single dose of 400 mg/kg artemether.

Author(s): 
Xiao, Shuhua
Shen, Binggui
Utzinger, Jürg
Chollet, Jacques
Tanner, Marcel

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