Microscopy, Electron, Transmission

Publication Title: 
Chemico-Biological Interactions

Mitochondria play a central role in molecular events leading to tissue damage in ischemia. The present study examines the role of the alcoholic extract of T. chebula (TCE) pretreatment (50 mg/100 g body weight) to attenuate the isoproterenol (ISO) (20mg/100g body wt, sc) induced alterations on heart mitochondrial ultrastucture and function in experimental rats.

Author(s): 
Suchalatha, Subramaniyan
Srinivasan, Periasamy
Devi, Chennam Srinivasulu Shyamala
Publication Title: 
Journal of Medical Microbiology

Shigella spp. (Shigella dysenteriae, Shigella flexneri, Shigella boydii and Shigella sonnei) cause bacillary dysentery (shigellosis), which is characterized by bloody mucous diarrhoea. Although a variety of antibiotics have been effective for treatment of shigellosis, options are becoming limited due to globally emerging drug resistance. In the present study, in vitro antibacterial activity of methyl gallate (MG) isolated from Terminalia chebula was determined by performing MIC, minimal bactericidal concentration (MBC) and time-kill kinetic studies.

Author(s): 
Acharyya, Saurabh
Sarkar, Prodipta
Saha, Dhira R.
Patra, Amarendra
Ramamurthy, T.
Bag, Prasanta K.
Publication Title: 
Homeopathy: The Journal of the Faculty of Homeopathy

BACKGROUND: Breast cancer is the most common cancer diagnosed among women and is the second leading cause of cancer death. Homeopathic medicines are part of the alternative medicines that are given as a supportive therapy in breast cancer. The objective of this study was to investigate the anticancer activity of commercially available homeopathic preparations of Terminalia chebula (TC) and evaluate their nanoparticulate nature.

Author(s): 
Wani, Kirtee
Shah, Nilesh
Prabhune, Asmita
Jadhav, Arun
Ranjekar, Prabhakar
Kaul-Ghanekar, Ruchika
Publication Title: 
Neurobiology of Disease

An increasing body of evidence indicates a role for oligomers of the amyloid-? peptide (A?) in the neurotoxicity of this peptide and the pathology of Alzheimer's disease (AD). Several neurotoxic oligomeric forms of A? have been noted ranging from the larger Amyloid ?-Derived Diffusible Ligands (ADDLs) to smaller trimers and dimers of A?. More recently a dodecameric form of A? with a 56 kDa molecular weight, denoted A?*56, was shown to cause memory impairment in AD model mice.

Author(s): 
Scherzer-Attali, R.
Farfara, D.
Cooper, I.
Levin, A.
Ben-Romano, T.
Trudler, D.
Vientrov, M.
Shaltiel-Karyo, R.
Shalev, D. E.
Segev-Amzaleg, N.
Gazit, E.
Segal, D.
Frenkel, D.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Dietary interventions are effective ways to extend or shorten lifespan. By examining midlife hepatic gene expressions in mice under different dietary conditions, which resulted in different lifespans and aging-related phenotypes, we were able to identify genes and pathways that modulate the aging process. We found that pathways transcriptionally correlated with diet-modulated lifespan and physiological changes were enriched for lifespan-modifying genes.

Author(s): 
Zhou, Bing
Yang, Liu
Li, Shoufeng
Huang, Jialiang
Chen, Haiyang
Hou, Lei
Wang, Jinbo
Green, Christopher D.
Yan, Zhen
Huang, Xun
Kaeberlein, Matt
Zhu, Li
Xiao, Huasheng
Liu, Yong
Han, Jing-Dong J.
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

We analyzed ultrastructural changes and markers of fission/fusion in hepatocyte mitochondria from mice submitted to 40% calorie restriction (CR) for 6 months versus ad-libitum-fed controls. To study the effects of dietary fat under CR, animals were separated into three CR groups with soybean oil (also in controls), fish oil, and lard. CR induced differential changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte.

Author(s): 
Khraiwesh, Husam
LÛpez-DomÌnguez, JosÈ A.
LÛpez-Lluch, Guillermo
Navas, Pl·cido
de Cabo, Rafael
Ramsey, Jon J.
Villalba, JosÈ M.
Gonz·lez-Reyes, JosÈ A.
Publication Title: 
The Journal of Biological Chemistry

At the core of Saccharomyces cerevisiae telomeres is an array of tandem telomeric DNA repeats bound site-specifically by multiple Rap1 molecules. There, Rap1 orchestrates the binding of additional telomere-associated proteins and negatively regulates both telomere fusion and length homeostasis. Using electron microscopy, viscosity, and light scattering measurements, we show that purified Rap1 is a monomer in solution that adopts a ringlike or C shape with a central cavity.

Author(s): 
Williams, Tanya L.
Levy, Daniel L.
Maki-Yonekura, Saori
Yonekura, Koji
Blackburn, Elizabeth H.
Publication Title: 
International Journal of Nanomedicine

We developed a novel method to prepare nanocapsules. A solute often crystallizes when its solubility alters from one solvent to another, and its firstborn crystals are used as templates to prepare nanocapsules for the first time, which is called firstborn microcrystallization method. By using this method, the maximum diameter of the nanocapsules including artesunate is about 76 nm, and wrapping state is well.

Author(s): 
Xiao, Xin-Cai
Hong, Zong-Guo
Publication Title: 
Ultrastructural Pathology

Ultrastructural changes to P. falciparum-infected red blood cells were examined in vitro after treatment with antimalarial drugs. Artesunate had the most rapid parasitocidal effect. All three drugs caused structural changes within the parasite, including dilatation of the parasitophorus vacuole membrane, depletion of ribosomes, mitochondrial swelling, and decreased formation of hemozoin crystals. The structure of surface knobs and Maurer's clefts were similar to controls but reduced in number. Only depletion of free ribosomes correlated with antimalarial drug exposure.

Author(s): 
Sachanonta, Navakanit
Chotivanich, Kesinee
Chaisri, Urai
Turner, Gareth D. H.
Ferguson, David J. P.
Day, Nicholas P. J.
Pongponratn, Emsri
Publication Title: 
PloS One

The objective of our study was to profile and compare the systematic changes between orally administered artesunate and intramuscularly injected artemether at a low dose over a 3-month period (92 consecutive days) in dogs. Intramuscular administration of 6 mg kg-1 artemether induced a decreased red blood cell (RBC) count (anemia), concurrent extramedullary hematopoiesis in the spleen and inhibition of erythropoiesis in the bone marrow.

Author(s): 
Yin, Ji-ye
Wang, He-mei
Wang, Quan-jun
Dong, Yan-sheng
Han, Gang
Guan, Yong-biao
Zhao, Ke-yong
Qu, Wen-sheng
Yuan, Ye
Gao, Xiao-xin
Jing, Shu-fang
Ding, Ri-gao

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