Muscle Fibers, Skeletal

Publication Title: 
Human Molecular Genetics

In amyotrophic lateral sclerosis (ALS), the progressive loss of motor neurons is accompanied by extensive muscle denervation, resulting in paralysis and ultimately death. Upregulation of amyloid beta (A4) precursor protein (APP) in muscle fibres coincides with symptom onset in both sporadic ALS patients and the SOD1(G93A) mouse model of familial ALS.

Bryson, J. Barney
Hobbs, Carl
Parsons, Michael J.
Bosch, Karen D.
Pandraud, Amelie
Walsh, Frank S.
Doherty, Patrick
Greensmith, Linda
Publication Title: 

Diminished mitochondrial oxidative phosphorylation and aerobic capacity are associated with reduced longevity. We tested whether resveratrol (RSV), which is known to extend lifespan, impacts mitochondrial function and metabolic homeostasis. Treatment of mice with RSV significantly increased their aerobic capacity, as evidenced by their increased running time and consumption of oxygen in muscle fibers.

Lagouge, Marie
Argmann, Carmen
Gerhart-Hines, Zachary
Meziane, Hamid
Lerin, Carles
Daussin, Frederic
Messadeq, Nadia
Milne, Jill
Lambert, Philip
Elliott, Peter
Geny, Bernard
Laakso, Markku
Puigserver, Pere
Auwerx, Johan
Publication Title: 

Adiponectin secreted from adipocytes binds to adiponectin receptors AdipoR1 and AdipoR2, and exerts antidiabetic effects via activation of AMPK and PPAR-? pathways, respectively. Levels of adiponectin in plasma are reduced in obesity, which causes insulin resistance and type 2 diabetes. Thus, orally active small molecules that bind to and activate AdipoR1 and AdipoR2 could ameliorate obesity-related diseases such as type 2 diabetes. Here we report the identification of orally active synthetic small-molecule AdipoR agonists.

Okada-Iwabu, Miki
Yamauchi, Toshimasa
Iwabu, Masato
Honma, Teruki
Hamagami, Ken-ichi
Matsuda, Koichi
Yamaguchi, Mamiko
Tanabe, Hiroaki
Kimura-Someya, Tomomi
Shirouzu, Mikako
Ogata, Hitomi
Tokuyama, Kumpei
Ueki, Kohjiro
Nagano, Tetsuo
Tanaka, Akiko
Yokoyama, Shigeyuki
Kadowaki, Takashi
Publication Title: 
Journal of Diabetes

BACKGROUND: Cinnamomum cassia (Family: Lauraceae) is an Ayurvedic medicinal plant used traditionally for the treatment of a number of diseases, including diabetes. The hypoglycemic effect of this plant has been established in vivo. However, the effects of cinnamic acid, isolated from C. cassia, on the insulin signaling cascade in an in vitro model have not been elucidated. Hence, the aim of the present study was to evaluate the anti-diabetic effect of cinnamic acid on glucose transport by L6 myotubes.

Lakshmi, Baddireddi Subhadra
Sujatha, Sundaresan
Anand, Singaravelu
Sangeetha, Kadapakkam Nandhabalan
Narayanan, Rangarajan Badri
Katiyar, Chandrakanth
Kanaujia, Anil
Duggar, Rajeev
Singh, Yogendra
Srinivas, Konasubrahmanya
Bansal, Vinay
Sarin, Simi
Tandon, Ruchi
Sharma, Suchitra
Singh, Suchita
Publication Title: 
American Journal of Physiology. Cell Physiology

Study has demonstrated an essential role of cortical filamentous actin (F-actin) in insulin-regulated glucose uptake by skeletal muscle. Here, we tested whether perturbations in F-actin contributed to impaired insulin responsiveness provoked by hyperinsulinemia. In L6 myotubes stably expressing GLUT4 that carries an exofacial myc-epitope tag, acute insulin stimulation (20 min, 100 nM) increased GLUT4myc translocation and glucose uptake by approximately 2-fold.

McCarthy, Alicia M.
Spisak, Kristen O.
Brozinick, Joseph T.
Elmendorf, Jeffrey S.
Publication Title: 
American Journal of Physiology. Endocrinology and Metabolism

Estrogen (E2) is reported to regulate skeletal muscle mass and contractile function; whether E2 exerts its effects through estrogen receptor-alpha (ERalpha) or -beta (ERbeta) is unclear. We determined the effect of ERalpha or ERbeta elimination on muscle mass and contractile function in multiple muscles of the lower limb, muscles with different locomotor tasks and proportions of fiber types I and II: soleus (Sol), plantaris (Plan), tibialis anterior (TA), and gastrocnemius (Gast) in mature female mice.

Brown, Marybeth
Ning, Jie
Ferreira, J. Andries
Bogener, Jennifer L.
Lubahn, Dennis B.
Publication Title: 

OBJECTIVE: We examined the role of butyric acid, a short-chain fatty acid formed by fermentation in the large intestine, in the regulation of insulin sensitivity in mice fed a high-fat diet. RESEARCH DESIGN AND METHODS: In dietary-obese C57BL/6J mice, sodium butyrate was administrated through diet supplementation at 5% wt/wt in the high-fat diet. Insulin sensitivity was examined with insulin tolerance testing and homeostasis model assessment for insulin resistance. Energy metabolism was monitored in a metabolic chamber.

Gao, Zhanguo
Yin, Jun
Zhang, Jin
Ward, Robert E.
Martin, Roy J.
Lefevre, Michael
Cefalu, William T.
Ye, Jianping
Publication Title: 
Biochimica Et Biophysica Acta

Infection with human immunodeficiency virus (HIV) and treatment with HIV-protease inhibitor (PI)-based highly active antiretroviral therapies (HAART) is associated with dysregulated fatty acid and lipid metabolism. Enhanced lipolysis, increased circulating fatty acid levels, and hepatic and intramuscular lipid accumulation appear to contribute to insulin resistance in HIV-infected people treated with PI-based HAART. However, it is unclear whether currently prescribed HIV-PIs directly alter skeletal muscle fatty acid transport, oxidation, and storage.

Richmond, Scott R.
Carper, Michael J.
Lei, Xiaoyong
Zhang, Sheng
Yarasheski, Kevin E.
Ramanadham, Sasanka
Publication Title: 
The Journal of Nutritional Biochemistry

Bioactive components from bitter melon (BM) have been reported to improve glucose metabolism in vivo, but definitive studies on efficacy and mechanism of action are lacking. We sought to investigate the effects of BM bioactives on body weight, muscle lipid content and insulin signaling in mice fed a high-fat diet and on insulin signaling in L6 myotubes. Male C57BL/6J mice were randomly divided into low-fat diet control (LFD), high-fat diet (HFD) and HFD plus BM (BM) groups.

Wang, Zhong Q.
Zhang, Xian H.
Yu, Yongmei
Poulev, Alexander
Ribnicky, David
Floyd, Z. Elizabeth
Cefalu, William T.
Publication Title: 
FASEB journal: official publication of the Federation of American Societies for Experimental Biology

Brassinosteroids are plant-derived polyhydroxylated derivatives of 5a-cholestane, structurally similar to cholesterol-derived animal steroid hormones and insect ecdysteroids, with no known function in mammals. 28-Homobrassinolide (HB), a steroidal lactone with potent plant growth-promoting property, stimulated protein synthesis and inhibited protein degradation in L6 rat skeletal muscle cells (EC(50) 4 μM) mediated in part by PI3K/Akt signaling pathway.

Esposito, Debora
Komarnytsky, Slavko
Shapses, Sue
Raskin, Ilya


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