Myocytes, Cardiac

Publication Title: 
Aging Cell

Fetal cardiomyocytes have been proposed as a potential source of cell-based therapy for heart failure. This study examined cellular senescence in cultured human fetal ventricular cardiomyocytes (HFCs). HFCs were isolated and identified by immunocytochemistry and RT-PCR. Cells were found to senesce after 20-25 population doublings, as determined by growth arrest, morphological changes and senescence-associated beta-galactosidase activity. Using the telomeric repeat amplification protocol assay, telomerase activity was undetectable in primary HFCs.

Author(s): 
Ball, Andrew J.
Levine, Fred
Publication Title: 
Disease Models & Mechanisms

Cardiac fibrosis is critically involved in the adverse remodeling accompanying dilated cardiomyopathies (DCMs), which leads to cardiac dysfunction and heart failure (HF). Connective tissue growth factor (CTGF), a profibrotic cytokine, plays a key role in this deleterious process. Some beneficial effects of IGF1 on cardiomyopathy have been described, but its potential role in improving DCM is less well characterized.

Author(s): 
Touvron, Melissa
Escoubet, Brigitte
Mericskay, Mathias
Angelini, Aude
Lamotte, Luciane
Santini, Maria Paola
Rosenthal, Nadia
Daegelen, Dominique
Tuil, David
Decaux, Jean-FranÁois
Publication Title: 
Laboratory Investigation; a Journal of Technical Methods and Pathology

The protective effect of heme oxygenase-1 (HO-1) expression in cardiovascular disease has been previously demonstrated using transgenic animal models in which HO-1 is constitutively overexpressed in the heart. However, the temporal requirements for protection by HO-1 induction relative to injury have not been investigated, but are essential to employ HO-1 as a therapeutic strategy in human cardiovascular disease states.

Author(s): 
Hull, Travis D.
Bolisetty, Subhashini
DeAlmeida, Angela C.
Litovsky, Silvio H.
Prabhu, Sumanth D.
Agarwal, Anupam
George, James F.
Publication Title: 
Acta Pharmacologica Sinica

AIM: To compare the effects of matrine, artemisinin, and tetrandrine on intracellular Ca2+ concentration ([Ca2+]i) in guinea pig ventricular myocytes. METHODS: A single ventricular myocyte was loaded with Fluo 3-acetoxymethyl (Fluo 3-AM). [Ca2+]i was recorded by laser scanning confocal microscope and represented by fluorescence intensity (FI). RESULTS: 1) KCl 60 mmol . L-1 elevated the FI from 299 +/ -19 to 1389 +/- 325 (P < 0.01) in the presence of extracellular Ca2+ 1.8 mmol . L-1. 2) Both matrine and artemisinin at the concentration of 100 micromol .

Author(s): 
Ai, J.
Gao, H. H.
He, S. Z.
Wang, L.
Luo, D. L.
Yang, B. F.
Publication Title: 
The Journal of Veterinary Medical Science / the Japanese Society of Veterinary Science

Concern has been growing about the cardiac toxicity of antimalarial drugs. Artemisinin, a unique type of antimalarial drug originating from a Chinese medicinal plant, has minimal adverse effects, but it has been reported to inhibit delayed rectifier potassium current, a voltage-gated potassium current. However, no studies have been published concerning the effect of artemisinin on ligand-gated potassium currents.

Author(s): 
Hara, Yukio
Yamawaki, Hideyuki
Shimada, Masami
Okada, Kiyohiro
Tanai, Toshiki
Ichikawa, Daiki
Miyake, Kimihito
Kizaki, Keiichiro
Publication Title: 
Phytomedicine: International Journal of Phytotherapy and Phytopharmacology

The bark of Terminalia arjuna (TA) has been used for centuries in ayurvedic medicine as cardiotonics for treatment of cardiac disorders. It became recently available as over-the-counter supplements marketed for maintaining a healthy heart. However, the cellular mechanism of its cardiotonic effect remains undefined. The present study was designed to investigate the physicochemical property and inotropic effect of the aqueous extract of TA bark (TA(AqE)) on adult rat ventricular myocytes in comparison with extracts prepared sequentially with organic solvents (organic extracts).

Author(s): 
Oberoi, Lalit
Akiyama, Toshiyuki
Lee, Kuo-Hsiung
Liu, Shi J.
Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: Stem bark gum resin extract of Boswellia serrata is traditionally used in India for its hemostatic, antiinflammatory and cardiovascular health effects and it is named as Śallakī in Ayurvedic medicine. AIM OF THE STUDY: This study was conducted to evaluate the antioxidative and antithrombotic properties of stem bark gum resin extracts of Boswellia serrata (BS). MATERIALS AND METHODS: The inhibitory activity of the BSWE and BSAE on FeCl(3) induced lipid peroxidation (in vitro) in rat liver and heart homogenates was measured spectrophotometrically.

Author(s): 
Kokkiripati, Praveen K.
Bhakshu, Lepakshi Md
Marri, Swathi
Padmasree, K.
Row, Anupama T.
Raghavendra, Agepati S.
Tetali, Sarada D.
Publication Title: 
Canadian Journal of Physiology and Pharmacology

Ayurveda is an Indian system of medicine. Despite clinical efficacy, lack of scientific validation has limited the effective use of Ayurvedic drugs. Cardoguard is an Ayurvedic antihypertensive drug formulated by Nagarjuna Herbal Concentrates Ltd., Kerala, India. Left ventricular hypertrophy (LVH) is a modifiable risk factor, and regression of LVH reduces the propensity for adverse cardiovascular events. This study was taken up with the objective of evaluating the efficacy of Cardoguard in the prevention of cardiac remodeling.

Author(s): 
Sankar, Vandana
Nair, Renuka R.
Harikrishnan, Vijayakumar S.
Fernandez, Adelaide C.
Kumar, Cherumanal S. Krishna
Madhavachandran, Viswanathamenon
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

In this study, we evaluated whether blocking myeloid differentiation factor-88 (MyD88) could decrease cardiac myocyte apoptosis following pressure overload. Adenovirus expressing dominant negative MyD88 (Ad5-dnMyD88) or Ad5-green fluorescent protein (GFP) (Ad5-GFP) was transfected into rat hearts (n = 8/group) immediately followed by aortic banding for 3 wk. One group of rats (n = 8) was subjected to aortic banding for 3 wk without transfection. Sham surgical operation (n = 8) served as control.

Author(s): 
Ha, Tuanzhu
Hua, Fang
Li, Yuehua
Ma, Jing
Gao, Xiang
Kelley, Jim
Zhao, Aiqiu
Haddad, Georges E.
Williams, David L.
Browder, I. William
Kao, Race L.
Li, Chuanfu
Publication Title: 
European Journal of Pharmacology

Since flavonoids scavenge reactive oxygen species, they may potentially protect against ischemia/reperfusion injury. This study compared the scavenging capacity of specific flavonoids towards different reactive oxygen species. Whether the differential oxidant scavenging capacity correlated with their protective efficacy in ischemia/reperfusion injury of cardiomyocytes was determined.

Author(s): 
Chang, Wei-Tien
Shao, Zuo-Hui
Yin, Jun-Jie
Mehendale, Sangeeta
Wang, Chong-Zhi
Qin, Yimin
Li, Juan
Chen, Wen-Jone
Chien, Chiang-Ting
Becker, Lance B.
Vanden Hoek, Terry L.
Yuan, Chun-Su

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