NIH 3T3 Cells

Publication Title: 
Cellular and molecular life sciences: CMLS

Reduction of nutrient intake without malnutrition positively influences lifespan and healthspan from yeast to mice and exerts some beneficial effects also in humans. The AMPK-FoxO axis is one of the evolutionarily conserved nutrient-sensing pathways, and the FOXO3A locus is associated with human longevity. Interestingly, FoxO3A has been reported to be also a mitochondrial protein in mammalian cells and tissues. Here we report that glucose restriction triggers FoxO3A accumulation into mitochondria of fibroblasts and skeletal myotubes in an AMPK-dependent manner.

Author(s): 
Peserico, Alessia
Chiacchiera, Fulvio
Grossi, Valentina
Matrone, Antonio
Latorre, Dominga
Simonatto, Marta
Fusella, Aurora
Ryall, James G.
Finley, Lydia W. S.
Haigis, Marcia C.
Villani, Gaetano
Puri, Pier Lorenzo
Sartorelli, Vittorio
Simone, Cristiano
Publication Title: 
Journal of Medicinal Chemistry

Two recently reported hit compounds, COR627 and COR628, underpinned the development of a series of 2-(acylamino)thiophene derivatives. Some of these compounds displayed significant activity in vitro as positive allosteric modulators of the GABAB receptor by potentiating GTP?S stimulation induced by GABA at 2.5 and 25 ?M while failing to exhibit intrinsic agonist activity. Compounds were also found to be effective in vivo, potentiating baclofen-induced sedation/hypnosis in DBA mice when administered either intraperitoneally or intragastrically.

Author(s): 
Mugnaini, Claudia
Pedani, Valentina
Casu, Angelo
Lobina, Carla
Casti, Alberto
Maccioni, Paola
Porcu, Alessandra
Giunta, Daniela
Lamponi, Stefania
Solinas, Maurizio
Dragoni, Stefania
Valoti, Massimo
Colombo, Giancarlo
Castelli, Maria Paola
Gessa, Gian Luigi
Corelli, Federico
Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: India is a rich source of medicinal plants and a number of plant extracts are used against diseases in various systems of medicine such as Ayurveda, Unani and Siddha. Only a few of them have been scientifically explored. One of such plants is Ammannia baccifera L., traditionally used as an antitumor agent. AIM OF THE STUDY: The objective of the present study was to explore the anticancer activity of the methanol extract of A.

Author(s): 
Loganayaki, Nataraj
Manian, Sellamuthu
Publication Title: 
The Journal of Neuroscience: The Official Journal of the Society for Neuroscience

Alzheimer's disease (AD) neuropathology is characterized by the accumulation of phosphorylated tau and amyloid-beta peptides derived from the amyloid precursor protein (APP). Elevated blood levels of homocysteine are a significant risk factor for many age-related diseases, including AD. Impaired homocysteine metabolism favors the formation of S-adenosylhomocysteine, leading to inhibition of methyltransferase-dependent reactions.

Author(s): 
Sontag, Estelle
Nunbhakdi-Craig, Viyada
Sontag, Jean-Marie
Diaz-Arrastia, Ramon
Ogris, Egon
Dayal, Sanjana
Lentz, Steven R.
Arning, Erland
Bottiglieri, Teodoro
Publication Title: 
American Journal of Respiratory Cell and Molecular Biology

Therapies to correct the ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) folding defect require sensitive methods to detect channel activity in vivo. The β₂ adrenergic receptor agonists, which provide the CFTR stimuli commonly used in nasal potential difference assays, may not overcome the channel gating defects seen in ΔF508 CFTR after plasma membrane localization. In this study, we identify an agent, quercetin, that enhances the detection of surface ΔF508 CFTR, and is suitable for nasal perfusion.

Author(s): 
Pyle, Louise C.
Fulton, Jennifer C.
Sloane, Peter A.
Backer, Kyle
Mazur, Marina
Prasain, Jeevan
Barnes, Stephen
Clancy, J. P.
Rowe, Steven M.
Publication Title: 
Arteriosclerosis, Thrombosis, and Vascular Biology

OBJECTIVE: Trivalent chromium (Cr3+) is an essential micronutrient. Findings since the 1950s suggest that Cr3+ might benefit cholesterol homeostasis. Here we present mechanistic evidence in support of this role of Cr3+. METHODS AND RESULTS: High-density lipoprotein cholesterol generation in 3T3-L1 adipocytes, which are rendered ineffective by the hyperinsulinemia that is known to accompany disorders of lipid metabolism, was corrected by Cr3+.

Author(s): 
Sealls, Whitney
Penque, Brent A.
Elmendorf, Jeffrey S.
Publication Title: 
The Journal of Biological Chemistry

Fibroblast growth factors are key proteins in many intercellular signaling networks. They normally remain attached to the extracellular matrix, which confers on them a considerable stability. The unrestrained accumulation of fibroblast growth factors in the extracellular milieu, either due to uncontrolled synthesis or enzymatic release, contributes to the pathology of many diseases. Consequently, the neutralization of improperly mobilized fibroblast growth factors is of clear therapeutic interest.

Author(s): 
Fernández, Israel S.
Cuevas, Pedro
Angulo, Javier
López-Navajas, Pilar
Canales-Mayordomo, Angeles
González-Corrochano, Rocío
Lozano, Rosa M.
Valverde, Serafín
Jiménez-Barbero, Jesús
Romero, Antonio
Giménez-Gallego, Guillermo
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Adjustable control of therapeutic transgenes in engineered cell implants after transdermal and topical delivery of nontoxic trigger molecules would increase convenience, patient compliance, and elimination of hepatic first-pass effect in future therapies. Pseudomonas putida DOT-T1E has evolved the flavonoid-triggered TtgR operon, which controls expression of a multisubstrate-specific efflux pump (TtgABC) to resist plant-derived defense metabolites in its rhizosphere habitat. Taking advantage of the TtgR operon, we have engineered a hybrid P.

Author(s): 
Gitzinger, Marc
Kemmer, Christian
El-Baba, Marie Daoud
Weber, Wilfried
Fussenegger, Martin
Publication Title: 
Cell

Metabolic adaptation is essential for cell survival during nutrient deprivation. We report that eukaryotic elongation factor 2 kinase (eEF2K), which is activated by AMP-kinase (AMPK), confers cell survival under acute nutrient depletion by blocking translation elongation. Tumor cells exploit this pathway to adapt to nutrient deprivation by reactivating the AMPK-eEF2K axis. Adaptation of transformed cells to nutrient withdrawal is severely compromised in cells lacking eEF2K.

Author(s): 
Leprivier, Gabriel
Remke, Marc
Rotblat, Barak
Dubuc, Adrian
Mateo, Abigail-Rachele F.
Kool, Marcel
Agnihotri, Sameer
El-Naggar, Amal
Yu, Bin
Somasekharan, Syam Prakash
Faubert, Brandon
Bridon, Gaëlle
Tognon, Cristina E.
Mathers, Joan
Thomas, Ryan
Li, Amy
Barokas, Adi
Kwok, Brian
Bowden, Mary
Smith, Stephanie
Wu, Xiaochong
Korshunov, Andrey
Hielscher, Thomas
Northcott, Paul A.
Galpin, Jason D.
Ahern, Christopher A.
Wang, Ye
McCabe, Martin G.
Collins, V. Peter
Jones, Russell G.
Pollak, Michael
Delattre, Olivier
Gleave, Martin E.
Jan, Eric
Pfister, Stefan M.
Proud, Christopher G.
Derry, W. Brent
Taylor, Michael D.
Sorensen, Poul H.
Publication Title: 
RNA (New York, N.Y.)

Cordycepin (3' deoxyadenosine) has long been used in the study of in vitro assembled polyadenylation complexes, because it terminates the poly(A) tail and arrests the cleavage complex. It is derived from caterpillar fungi, which are highly prized in Chinese traditional medicine. Here we show that cordycepin specifically inhibits the induction of inflammatory mRNAs by cytokines in human airway smooth muscle cells without affecting the expression of control mRNAs.

Author(s): 
Kondrashov, Alexander
Meijer, Hedda A.
Barthet-Barateig, Adeline
Parker, Hannah N.
Khurshid, Asma
Tessier, Sarah
Sicard, Marie
Knox, Alan J.
Pang, Linhua
De Moor, Cornelia H.

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