Nuclear Magnetic Resonance, Biomolecular

Publication Title: 
Planta Medica

Phytochemical investigations of Terminalia arjuna bark and Terminalia chebula fruits resulted in the isolation of twelve triterpenoids including two new oleanane type triterpene glucosyl esters, ajunglucosides IV (1) and V (2), from the N-BuOH layer of the MeOH extract of T. arjuna bark as well as nine oleanane-type triterpenoids from the MeOH extract of the fruits of T. chebula.

Author(s): 
Wang, Wei
Ali, Zulfiqar
Li, Xing-Cong
Shen, Yunheng
Khan, Ikhlas A.
Publication Title: 
Journal of Asian Natural Products Research

Two new triterpenoids, termichebuloside A (1), an unusual dimeric triterpenoid saponin, and termichebulolide (2), an oleanolic acid-type lactone, along with 11 known triterpenoids, were isolated from MeOH extract of the barks of Terminalia chebula. The structures of 1 and 2 were elucidated to be arjunglucoside I-(3-O-19',23-O-19')-18,19-seco-19-hydroxyarjunglucoside I (1) and 2?,3?,23-trihydroxyolean-11,13(18)-dien-28,19?-olide (2), respectively, on the basis of spectroscopic evidences and biogenetic consideration.

Author(s): 
Zhang, Chao
Jiang, Kun
Qu, Shi-Jin
Zhai, Yi-Ming
Tan, Jun-Jie
Tan, Chang-Heng
Publication Title: 
Journal of Proteome Research

Long-term restriction of energy intake without malnutrition is a robust intervention that has been shown to prolong life and delay age-related morbidity. A 1H NMR-based metabonomic strategy was used to monitor urinary metabolic profiles throughout the lifetimes of control-fed and diet-restricted dogs. Urinary metabolic trajectories were constructed for each dog, and metabolic variation was found to be predominantly influenced by age. Urinary excretion of creatinine increased with age, reaching a maximum between ages 5 and 9 years and declining thereafter.

Author(s): 
Wang, Yulan
Lawler, Dennis
Larson, Brian
Ramadan, Ziad
Kochhar, Sunil
Holmes, Elaine
Nicholson, Jeremy K.
Publication Title: 
Journal of Proteome Research

Modeling aging and age-related pathologies presents a substantial analytical challenge given the complexity of gene-environment influences and interactions operating on an individual. A top-down systems approach is used to model the effects of lifelong caloric restriction, which is known to extend life span in several animal models.

Author(s): 
Richards, Selena E.
Wang, Yulan
Claus, Sandrine P.
Lawler, Dennis
Kochhar, Sunil
Holmes, Elaine
Nicholson, Jeremy K.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Telomerase is a ribonucleoprotein (RNP) required for maintenance of telomeres. Although up-regulated telomerase activity is closely linked to the cellular immortality characteristic of late stage carcinogenesis, recently, mutations in the telomerase RNA gene in humans have been associated with dyskeratosis congenita and aplastic anemia, both typified by impaired haemopoietic function. These mutations include base changes in a highly conserved putative telomerase RNA pseudoknot.

Author(s): 
Comolli, Luis R.
Smirnov, Ivan
Xu, Lifeng
Blackburn, Elizabeth H.
James, Thomas L.
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis

Sesamum (Sesamum indicum) seed and its oil have been in use in Indian traditional medicine, 'Ayurveda' since antiquity. However, there has been no attempt to standardize the polyherbal formulations containing sesamum oil as the main ingredient in terms of its active principle or marker compound. Biologically active lignans in sesamum oil are identified as the marker compound for the oil and its formulations.

Author(s): 
Sukumar, Divya
Arimboor, Ranjith
Arumughan, C.
Publication Title: 
Xenobiotica; the Fate of Foreign Compounds in Biological Systems

Andrographolide is a major labdane diterpenoid of the traditional Chinese and Ayurvedic medicine. Andrographis paniculate (Burm) Nees, is used in clinical situations in China mainly to treat fever, cold, and inflammation. In our previous study, fifteen metabolites of andrographolide were identified in human urine. However, there are still two other unknown metabolites. The aim of this study was to elucidate the structures of these two metabolites. 3.

Author(s): 
Qiu, Feng
Cui, Liang
Chen, Lixia
Sun, Jiawen
Yao, Xinsheng
Publication Title: 
Journal of Natural Products

Quantitative 1H NMR (qHNMR) provides a value-added dimension to the standard spectroscopic data set involved in structure analysis, especially when analyzing bioactive molecules and elucidating new natural products. The qHNMR method can be integrated into any routine qualitative workflow without much additional effort by simply establishing quantitative conditions for the standard solution 1H NMR experiments.

Author(s): 
Pauli, Guido F.
Jaki, Birgit U.
Lankin, David C.
Publication Title: 
Journal of Natural Products

Extracts from the roots and rhizomes of black cohosh (Cimicifuga racemosa) are widely used as dietary supplements to alleviate menopausal symptoms. State-of-the-art quality control measures involve phytochemical fingerprinting of the triterpene glycosides for species identification and chemical standardization by HPLC. In the course of developing materials and methods for standardization procedures, the major C. racemosa triterpene glycoside (1) was isolated and initially thought to be cimicifugoside (2).

Author(s): 
Chen, Shao-Nong
Lankin, David C.
Nikolic, Dejan
Fabricant, Daniel S.
Lu, Zhi-Zhen
Ramirez, Benjamin
van Breemen, Richard B.
Fong, Harry H. S.
Farnsworth, Norman R.
Pauli, Guido F.
Publication Title: 
Journal of Natural Products

Monomeric phthalides such as Z-ligustilide (1) and Z-butylidenephthalide (2) are major constituents of medicinal plants of the Apiaceae family. While 1 has been associated with a variety of observed biological effects, it is also known for its instability and rapid chemical degradation. For the purpose of isolating pure 1 and 2, a gentle and rapid two-step countercurrent isolation procedure was developed.

Author(s): 
Schinkovitz, Andreas
Pro, Samuel M.
Main, Matthew
Chen, Shao-Nong
Jaki, Birgit U.
Lankin, David C.
Pauli, Guido F.

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