Nuclear Proteins

Publication Title: 
PloS One

BACKGROUND: The typical Western diet is not balanced in methyl nutrients that regulate the level of the methyl donor S-adenosylmethionine (SAM) and its derivative metabolite S-adenosylhomocysteine (SAH), which in turn may control the activity of certain methyltransferases. Feeding rodents with amino acid defined and methyl-imbalanced diet decreases hepatic SAM and causes liver cancers. RIZ1 (PRDM2 or KMT8) is a tumor suppressor and functions in transcriptional repression by methylating histone H3 lysine 9.

Zhou, Wenyun
Alonso, Sergio
Takai, Daisaku
Lu, Shelly C.
Yamamoto, Fumiichiro
Perucho, Manuel
Huang, Shi
Publication Title: 
International Journal of Cancer. Journal International Du Cancer

Gene promoter CpG island hypermethylation is associated with Helicobacter pylori (H. pylori) infection and may be an important initiator of gastric carcinogenesis. To examine factors influencing methylation, we utilized bisulfite Pyrosequencing® technology for quantitative analysis of promoter DNA methylation in RPRM, APC, MGMT and TWIST1 genes using DNA from 86 gastric biopsies from Colombian residents of areas with high and low incidence of gastric cancer. H. pylori colonies were cultured from the same subjects, and gastric pathology was evaluated.

Schneider, Barbara G.
Peng, Dun-Fa
Camargo, M. Constanza
Piazuelo, M. Blanca
Sicinschi, Liviu A.
Mera, Robertino
Romero-Gallo, Judith
Delgado, Alberto G.
Bravo, Luis E.
Wilson, Keith T.
Peek, Richard M.
Correa, Pelayo
El-Rifai, Wael
Publication Title: 
Developmental Biology

The retinoblastoma gene Rb is the prototype tumor suppressor and is conserved in Drosophila. We use the developing fly retina as a model system to investigate the role of Drosophila Rb (rbf) during differentiation. This report shows that mutation of rbf and rhinoceros (rno), which encodes a PHD domain protein, leads to a synergistic delay in photoreceptor cell differentiation in the developing eye disc.

Sukhanova, Madina J.
Steele, Latishya J.
Zhang, Tianyi
Gordon, Gabriel M.
Du, Wei
Publication Title: 
Toxicological Sciences: An Official Journal of the Society of Toxicology

Although crosstalk between aryl hydrocarbon receptor (AhR) and estrogen receptor α (ERα) is well established, the mechanistic basis and involvement of other proteins in this process are not known. Because we observed an enrichment of AhR-binding motifs in ERα-binding sites of many estradiol (E2)-regulated genes, we investigated how AhR might modulate ERα-mediated gene transcription in breast cancer cells. Gene regulations were categorized based on their pattern of stimulation by E2 and/or dioxin and were denoted E2-responsive, dioxin-responsive, or responsive to either ligand.

Madak-Erdogan, Zeynep
Katzenellenbogen, Benita S.
Publication Title: 
Stem Cells and Development

We have previously demonstrated that hypoxia stimulates adipose-derived stem cells (ASCs) through the generation of reactive oxygen species (ROS). However, the precise mechanism involved in the ROS generation by ASCs is not well understood. We sought to investigate in this work: (1) which subtype of NADPH oxidase (Nox) is primarily expressed in ASCs; (2) where Nox4 is localized in ASCs; and (3) whether silencing of Nox4 attenuates hypoxia-enhanced function of ASC.

Kim, Ji Hye
Song, Seung-Yong
Park, Sang Gyu
Song, Sun U.
Xia, Ying
Sung, Jong-Hyuk
Publication Title: 

OBJECTIVE: The myeloid translocation genes (MTGs) are transcriptional corepressors with both Mtg8(-/-) and Mtgr1(-/-) mice showing developmental and/or differentiation defects in the intestine. We sought to determine the role of MTG16 in intestinal integrity. METHODS: Baseline and stress induced colonic phenotypes were examined in Mtg16(-/-) mice. To unmask phenotypes, we treated Mtg16(-/-) mice with dextran sodium sulphate (DSS) or infected them with Citrobacter rodentium and the colons were examined for ulceration and for changes in proliferation, apoptosis and inflammation.

Williams, Christopher S.
Bradley, Amber M.
Chaturvedi, Rupesh
Singh, Kshipra
Piazuelo, Maria B.
Chen, Xi
McDonough, Elizabeth M.
Schwartz, David A.
Brown, Caroline T.
Allaman, Margaret M.
Coburn, Lori A.
Horst, Sara N.
Beaulieu, Dawn B.
Choksi, Yash A.
Washington, Mary Kay
Williams, Amanda D.
Fisher, Melissa A.
Zinkel, Sandra S.
Peek, Richard M.
Wilson, Keith T.
Hiebert, Scott W.
Publication Title: 
Oncology Reports

Plant-derived active constituents and their semi-synthetic or synthetic analogs have served as major sources of anticancer drugs. 20(S)-protopanaxadiol (PPD) is a metabolite of ginseng saponin of both American ginseng (Panax quinquefolius L.) and Asian ginseng (Panax ginseng C.A. Meyer). We previously demonstrated that ginsenoside Rg3, a glucoside precursor of PPD, exhibits anti-proliferative effects on HCT116 cells and reduces tumor size in a xenograft model.

Gao, Jian-Li
Lv, Gui-Yuan
He, Bai-Cheng
Zhang, Bing-Qiang
Zhang, Hongyu
Wang, Ning
Wang, Chong-Zhi
Du, Wei
Yuan, Chun-Su
He, Tong-Chuan
Publication Title: 
Molecular Systems Biology

The closely related transcription factors (TFs), estrogen receptors ERα and ERβ, regulate divergent gene expression programs and proliferative outcomes in breast cancer. Utilizing breast cancer cells with ERα, ERβ, or both receptors as a model system to define the basis for differing response specification by related TFs, we show that these TFs and their key coregulators, SRC3 and RIP140, generate overlapping as well as unique chromatin-binding and transcription-regulating modules.

Madak-Erdogan, Zeynep
Charn, Tze-Howe
Jiang, Yan
Liu, Edison T.
Katzenellenbogen, John A.
Katzenellenbogen, Benita S.
Publication Title: 
FASEB journal: official publication of the Federation of American Societies for Experimental Biology

Because little is known about the actions of botanical estrogens (BEs), widely consumed by menopausal women, we investigated the mechanistic and cellular activities of some major BEs. We examined the interactions of genistein, daidzein, equol, and liquiritigenin with estrogen receptors ERα and ERβ, with key coregulators (SRC3 and RIP140) and chromatin binding sites, and the regulation of gene expression and proliferation in MCF-7 breast cancer cells containing ERα and/or ERβ.

Jiang, Yan
Gong, Ping
Madak-Erdogan, Zeynep
Martin, Teresa
Jeyakumar, Muthu
Carlson, Kathryn
Khan, Ikhlas
Smillie, Troy J.
Chittiboyina, Amar G.
Rotte, Sateesh C. K.
Helferich, William G.
Katzenellenbogen, John A.
Katzenellenbogen, Benita S.
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

Strong epidemiological and experimental evidence indicate that hypertension in the elderly predisposes to the development of Alzheimer's disease (AD), but the underlying mechanisms remain elusive. The present study was designed to characterize the additive/synergistic effects of hypertension and aging on the expression of genes involved in β-amyloid generation and AD in the hippocampus, an area of brain contributing to higher cognitive function, which is significantly affected by AD both in humans and in mouse models of the disease.

Csiszar, Anna
Tucsek, Zsuzsanna
Toth, Peter
Sosnowska, Danuta
Gautam, Tripti
Koller, Akos
Deak, Ferenc
Sonntag, William E.
Ungvari, Zoltan


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