Oligodeoxyribonucleotides

Publication Title: 
Genes & Development

The ribonucleoprotein (RNP) enzyme telomerase from Saccharomyces cerevisiae adds telomeric DNA to chromosomal ends in short increments both in vivo and in vitro. Whether or not telomerase functions as a multimer has not been addressed previously. Here we show, first, that following polymerization, the telomerase RNP remains stably bound to its telomeric oligonucleotide reaction product. We then exploit this finding and a previously reported mutant telomerase RNA to demonstrate that, unexpectedly, the S.

Author(s): 
Prescott, J.
Blackburn, E. H.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Macronuclear telomeres in Oxytricha exist as DNA-protein complexes in which the termini of the G-rich strands are bound by a 97-kDa telomere protein. During telomeric DNA replication, the replication machinery must have access to the G-rich strand. However, given the stability of telomere protein binding, it has been unclear how this is accomplished. In this study we investigated the ability of several different DNA polymerases to access telomeric DNA in Oxytricha telomere protein-DNA complexes.

Author(s): 
Shippen, D. E.
Blackburn, E. H.
Price, C. M.
Publication Title: 
Cell

We have analyzed the de novo telomere synthesis catalyzed by the enzyme telomere terminal transferase (telomerase) from Tetrahymena. Oligonucleotides representing the G-rich strand of telomeric sequences from five different organisms specifically primed the addition of TTGGGG repeats in vitro, suggesting that primer recognition may involve a DNA structure unique to these oligonucleotides. The sequence at the 3' end of the oligonucleotide primer specified the first nucleotide added in the reaction.

Author(s): 
Greider, C. W.
Blackburn, E. H.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Air, G. M.
Blackburn, E. H.
Coulson, A. R.
Galibert, F.
Sanger, F.
Sedat, J. W.
Ziff, E. B.
Publication Title: 
Cell

Developmentally controlled genomic deletion-ligations occur during ciliate macronuclear differentiation. We have identified a novel activity in Tetrahymena cell-free extracts that efficiently catalyzes a specific set of intramolecular DNA deletion-ligation reactions. When synthetic DNA oligonucleotide substrates were used, all the deletion-ligation products resembled those formed in vivo in that they resulted from deletions between pairs of short direct repeats. The reaction is ATP-dependent, salt-sensitive, and strongly influenced by the oligonucleotide substrate sequence.

Author(s): 
Robinson, E. K.
Cohen, P. D.
Blackburn, E. H.
Publication Title: 
Cell

Structural properties of DNA oligonucleotides corresponding to the single-stranded molecular terminus of telomeres from several organisms were analyzed. Based on physical studies including nondenaturing polyacrylamide gel electrophoresis, absorbance thermal denaturation analysis, and 1H and 31P nuclear magnetic resonance spectroscopy, we conclude that these molecules can self-associate by forming non-Watson-Crick, guanine.guanine based-paired, intramolecular structures.

Author(s): 
Henderson, E.
Hardin, C. C.
Walk, S. K.
Tinoco, I.
Blackburn, E. H.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Air, G. M.
Blackburn, E. H.
Sanger, F.
Coulson, A. R.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Macronuclear telomeres in Oxytricha exist as DNA-protein complexes in which the termini of the G-rich strands are bound by a 97-kDa telomere protein. During telomeric DNA replication, the replication machinery must have access to the G-rich strand. However, given the stability of telomere protein binding, it has been unclear how this is accomplished. In this study we investigated the ability of several different DNA polymerases to access telomeric DNA in Oxytricha telomere protein-DNA complexes.

Author(s): 
Shippen, D. E.
Blackburn, E. H.
Price, C. M.
Publication Title: 
Cell

Structural properties of DNA oligonucleotides corresponding to the single-stranded molecular terminus of telomeres from several organisms were analyzed. Based on physical studies including nondenaturing polyacrylamide gel electrophoresis, absorbance thermal denaturation analysis, and 1H and 31P nuclear magnetic resonance spectroscopy, we conclude that these molecules can self-associate by forming non-Watson-Crick, guanine.guanine based-paired, intramolecular structures.

Author(s): 
Henderson, E.
Hardin, C. C.
Walk, S. K.
Tinoco, I.
Blackburn, E. H.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Air, G. M.
Blackburn, E. H.
Coulson, A. R.
Galibert, F.
Sanger, F.
Sedat, J. W.
Ziff, E. B.

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