Publication Title: 
Journal of Natural Products

Four new partially acylated tetrasaccharides of 11-hydroxyhexadecanoic acid (1-4) were isolated from a methanolic extract of Ipomoea tyrianthina. The structures of these compounds were elucidated by spectroscopic and chemical methods. The resin glycoside composition of I. tyrianthina varied with the location of growth in Mexico.

León-Rivera, Ismael
Mirón-López, Gumersindo
Molina-Salinas, Gloria María
Herrera-Ruiz, Maribel
Estrada-Soto, Samuel
del Carmen Gutiérrez, María
Alonso-Cortes, Daniel
Navarrete-Vázquez, Gabriel
Rios, María Yolanda
Said-Fernández, Salvador
Publication Title: 
International Journal of Biological Macromolecules

Asparagus racemosus Linn. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant. In the present study, aqueous extract of A. racemosus (ARC) was fractionated and screened for the polysaccharide fraction (ARP). The characterization was done by enzymatic, Size Exclusion, gas chromatography with flame ionization detector (GC-FID), high pressure anion exchange chromatography (HPAEC) and thin layer chromatographic analyses. Phyto-chemical evaluation confirmed the presence of 26.7% of 2→1 linked fructo-oligosaccharides (FOS).

Thakur, Mayank
Connellan, Paul
Deseo, Myrna A.
Morris, Carol
Praznik, Werner
Loeppert, Renate
Dixit, V. K.
Publication Title: 
International Journal of Oncology

Glycosylation of proteins plays multiple roles in cell-cell and cell-matrix interactions. Fucose is a monosaccharide associated with glycosylation events and is known to be over-expressed in many malignant tumors. By using alpha-L-fucosidase (alpha-L-fase), a glycosidase that specifically removes alpha-L-fucose (alpha-L-f), we have examined the potential effects of defucosylation on tumor functions, focusing on tumor progression in the context of the interaction of tumor cells with the extracellular microenvironment.

Yuan, Kun
Kucik, Dennis
Singh, Raj K.
Listinsky, Catherine M.
Listinsky, Jay J.
Siegal, Gene P.
Publication Title: 
JPEN. Journal of parenteral and enteral nutrition

BACKGROUND: Sepsis and septic shock syndrome are the leading causes of death in critically ill patients. Lipopolysaccharide (LPS) released by the colonic microorganisms may translocate across a compromised lumen, leading to upregulated reactive oxidative stress, inflammation, and sepsis. The authors examined an enteral formula high in cysteine (antioxidant precursor), omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and prebiotic fructooligosaccharides (FOS) against systemic inflammatory syndrome.

Oz, Helieh S.
Chen, Theresa S.
Neuman, Manuela
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

How cancer cells bind to vascular surfaces and extravasate into target organs is an underappreciated, yet essential step in metastasis. We postulate that the metastatic process involves discrete adhesive interactions between circulating cancer cells and microvascular endothelial cells. Sialyl Lewis X (sLe(X)) on prostate cancer (PCa) cells is thought to promote metastasis by mediating PCa cell binding to microvascular endothelial (E)-selectin. Yet, regulation of sLe(X) and related E-selectin ligand expression in PCa cells is a poorly understood factor in PCa metastasis.

Barthel, Steven R.
Wiese, Georg K.
Cho, Jaehyung
Opperman, Matthew J.
Hays, Danielle L.
Siddiqui, Javed
Pienta, Kenneth J.
Furie, Bruce
Dimitroff, Charles J.
Publication Title: 
The Journal of Biological Chemistry

Prior studies have shown that treatment with the peracetylated 4-fluorinated analog of glucosamine (4-F-GlcNAc) elicits anti-skin inflammatory activity by ablating N-acetyllactosamine (LacNAc), sialyl Lewis X (sLe(X)), and related lectin ligands on effector leukocytes. Based on anti-sLe(X) antibody and lectin probing experiments on 4-F-GlcNAc-treated leukocytes, it was hypothesized that 4-F-GlcNAc inhibited sLe(X) formation by incorporating into LacNAc and blocking the addition of galactose or fucose at the carbon 4-position of 4-F-GlcNAc.

Barthel, Steven R.
Antonopoulos, Aristotelis
Cedeno-Laurent, Filiberto
Schaffer, Lana
Hernandez, Gilberto
Patil, Shilpa A.
North, Simon J.
Dell, Anne
Matta, Khushi L.
Neelamegham, Sriram
Haslam, Stuart M.
Dimitroff, Charles J.
Publication Title: 
Journal of Pediatric Gastroenterology and Nutrition

OBJECTIVES: Human milk oligosaccharides (HMOs) are the third most abundant component of breast milk. Our laboratory has previously revealed gene clusters specifically linked to HMO metabolism in selected bifidobacteria isolated from fecal samples of infants. Our objective was to test the hypothesis that growth of selected bifidobacteria on HMO stimulates the intestinal epithelium. METHODS: Caco-2 and HT-29 cells were incubated with lactose (LAC)- or HMO-grown Bifidobacterium longum subsp infantis (B infantis) or B bifidum.

Chichlowski, Maciej
De Lartigue, Guillaume
German, J. Bruce
Raybould, Helen E.
Mills, David A.
Publication Title: 
Cellular Immunology

The differentiation of T helper (Th) cells is critically dependent on cytokine milieu. The innate immune monocytes produce IL-1β which can affect the development of Th17 and Th1 cells that predominantly produce IL-17 and IFN-γ, respectively. Oligosaccharides from microorganisms, crops and mushrooms can stimulate innate immune cells. Active Hexose Correlated Compound (AHCC) that contains a large amount of oligosaccharides is a natural extract prepared from the mycelium of the edible Basidiomycete fungus.

Lee, Won-Woo
Lee, Naeun
Fujii, Hajime
Kang, Insoo
Publication Title: 
Advances in Nutrition (Bethesda, Md.)

Bifidobacteria are commonly used as probiotics in dairy foods. Select bifidobacterial species are also early colonizers of the breast-fed infant colon; however, the mechanism for this enrichment is unclear. We previously showed that Bifidobacterium longum subsp. infantis is a prototypical bifidobacterial species that can readily utilize human milk oligosaccharides as the sole carbon source. MS-based glycoprofiling has revealed that numerous B. infantis strains preferentially consume small mass oligosaccharides, abundant in human milks. Genome sequencing revealed that B.

Garrido, Daniel
Barile, Daniela
Mills, David A.
Publication Title: 
Food Microbiology

Prebiotics are non-digestible substrates that stimulate the growth of beneficial microbial populations in the intestine, especially Bifidobacterium species. Among them, fructo- and galacto-oligosaccharides are commonly used in the food industry, especially as a supplement for infant formulas. Mechanistic details on the enrichment of bifidobacteria by these prebiotics are important to understand the effects of these dietary interventions. In this study the consumption of galactooligosaccharides was studied for 22 isolates of Bifidobacterium longum subsp.

Garrido, Daniel
Ruiz-Moyano, Santiago
Jimenez-Espinoza, Rogelio
Eom, Hyun-Ju
Block, David E.
Mills, David A.


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