Osmotic Pressure

Publication Title: 
PloS One

The chronological lifespan of eukaryotic organisms is extended by the mutational inactivation of conserved growth-signaling pathways that regulate progression into and through the cell cycle. Here we show that in the budding yeast S. cerevisiae, these and other lifespan-extending conditions, including caloric restriction and osmotic stress, increase the efficiency with which nutrient-depleted cells establish or maintain a cell cycle arrest in G1.

Author(s): 
Weinberger, Martin
Feng, Li
Paul, Anita
Smith, Daniel L.
Hontz, Robert D.
Smith, Jeffrey S.
Vujcic, Marija
Singh, Keshav K.
Huberman, Joel A.
Burhans, William C.
Publication Title: 
The Journal of Biological Chemistry

The life span of model organisms can be modulated by environmental conditions that influence cellular metabolism, oxidation, or DNA integrity. The yeast nicotinamidase gene pnc1 was identified as a key transcriptional target and mediator of calorie restriction and stress-induced life span extension. PNC1 is thought to exert its effect on yeast life span by modulating cellular nicotinamide and NAD levels, resulting in increased activity of Sir2 family class III histone deacetylases.

Author(s): 
Balan, Vitaly
Miller, Gregory S.
Kaplun, Ludmila
Balan, Karina
Chong, Zhao-Zhong
Li, Faqi
Kaplun, Alexander
VanBerkum, Mark F. A.
Arking, Robert
Freeman, D. Carl
Maiese, Kenneth
Tzivion, Guri
Publication Title: 
Cell Cycle (Georgetown, Tex.)

Our studies revealed that LCA (lithocholic bile acid) extends yeast chronological lifespan if added to growth medium at the time of cell inoculation. We also demonstrated that longevity in chronologically aging yeast is programmed by the level of metabolic capacity and organelle organization that they developed before entering a quiescent state and, thus, that chronological aging in yeast is likely to be the final step of a developmental program progressing through at least one checkpoint prior to entry into quiescence.

Author(s): 
Burstein, Michelle T.
Kyryakov, Pavlo
Beach, Adam
Richard, Vincent R.
Koupaki, Olivia
Gomez-Perez, Alejandra
Leonov, Anna
Levy, Sean
Noohi, Forough
Titorenko, Vladimir I.
Publication Title: 
Journal of Cellular Biochemistry

Endothelin-1 (ET-1) disrupts insulin-regulated glucose transporter GLUT4 trafficking. Since the negative consequence of chronic ET-1 exposure appears to be independent of signal disturbance along the insulin receptor substrate-1/phosphatidylinositol (PI) 3-kinase (PI3K)/Akt-2 pathway of insulin action, we tested if ET-1 altered GLUT4 regulation engaged by osmotic shock, a PI3K-independent stimulus that mimics insulin action. Regulation of GLUT4 by hyperosmotic stress was impaired by ET-1.

Author(s): 
Strawbridge, Andrew B.
Elmendorf, Jeffrey S.
Publication Title: 
PloS One

The chronological lifespan of eukaryotic organisms is extended by the mutational inactivation of conserved growth-signaling pathways that regulate progression into and through the cell cycle. Here we show that in the budding yeast S. cerevisiae, these and other lifespan-extending conditions, including caloric restriction and osmotic stress, increase the efficiency with which nutrient-depleted cells establish or maintain a cell cycle arrest in G1.

Author(s): 
Weinberger, Martin
Feng, Li
Paul, Anita
Smith, Daniel L.
Hontz, Robert D.
Smith, Jeffrey S.
Vujcic, Marija
Singh, Keshav K.
Huberman, Joel A.
Burhans, William C.
Publication Title: 
Cell Cycle (Georgetown, Tex.)

Our studies revealed that LCA (lithocholic bile acid) extends yeast chronological lifespan if added to growth medium at the time of cell inoculation. We also demonstrated that longevity in chronologically aging yeast is programmed by the level of metabolic capacity and organelle organization that they developed before entering a quiescent state and, thus, that chronological aging in yeast is likely to be the final step of a developmental program progressing through at least one checkpoint prior to entry into quiescence.

Author(s): 
Burstein, Michelle T.
Kyryakov, Pavlo
Beach, Adam
Richard, Vincent R.
Koupaki, Olivia
Gomez-Perez, Alejandra
Leonov, Anna
Levy, Sean
Noohi, Forough
Titorenko, Vladimir I.
Publication Title: 
Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan

A novel two-step release system for the traditional Chinese medicine compound Danshen was developed by combining an effervescent osmotic pump tablet (EOPT) and a pulsed-released tablet (PT) of compound Danshen into one hard capsule. The EOPT of Danshen was prepared with sodium chloride, mannitol, hydroxypropylmethylcellulose (HPMC), and sodium bicarbonate as osmotic agents. The osmotic pressure from EOPT was greatly enhanced by carbon dioxide generated from the reaction between sodium bicarbonate and acidic components from Danshen.

Author(s): 
Li, Yuanbo
Hou, Shixiang
Bi, Yueqi
Zheng, Yu
Cai, Zheng
Cheng, Qiuhong
Song, Xiangrong
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