Publication Title: 
Archives of Biochemistry and Biophysics
Blackburn, E. H.
Hird, F. J.
Jones, I. K.
Publication Title: 
Revista Española De Anestesiología Y Reanimación

In this prospective study, 20 patients undergoing mean duration (2-3 h) neurosurgical operations on fossa cranii posterior, and cervical and dorsolumbar rachis, were induced with 0.3 mg/kg etomidate bolus dose. To maintain anesthesia, etomidate perfusions at 10 micrograms/kg/min (group I) and 20 microg/kg/min (group II) were administered. Fentanyl at fractionated doses was used as analgesic without association to nitrous oxide and relaxation was achieved with pancuronium bromide.

Fernández Galinski, S.
Barrera, E.
de Córdoba, J. L.
Covas, M. I.
Esquerdá, A.
Espinosa, W.
Publication Title: 
British Journal of Pharmacology

1. The clearance of dihydroartemisinin (DHA) in control and malaria-infected (MI) rats was investigated using the isolated perfused rat liver (IPRL) model and hepatic microsomal studies. 2. In the recirculating IPRL, clearance of DHA was reduced from a mean (s.d.) of 8.2+/-1.8 ml min(-1) in controls (n=8) to 6.0+/-1.0 ml min(-1) in MI (n=8; P<0.01). Clearance in control livers was similar to the perfusion flow rate, suggesting a high hepatic extraction ratio for DHA. 3.

Batty, K. T.
Ilett, K. F.
Edwards, G.
Powell, S. M.
Maggs, J. L.
Park, B. K.
Davis, T. M.
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

The objective of this study was to investigate whether the decrease in artemisinin bioavailability after repeated oral dosing in humans can be a result of increased efflux of artemisinin by P-glycoprotein or decreased membrane transport at the intestinal barrier. The effective jejunal permeability (Peff) of artemisinin was investigated using an in situ rat perfusion model. Fifty-four rats were randomized to one of three treatment arms: no pretreatment, pretreatment with artemisinin emulsion for 5 days (60 mg/kg/day, p.o. ), or pretreatment with emulsion vehicle for 5 days.

Svensson, U. S.
Sandström, R.
Carlborg, O.
Lennernäs, H.
Ashton, M.
Publication Title: 
Kidney International

BACKGROUND: Sodium artesunate is currently used in malaria treatment. Adverse effects of this drug have not been described, probably because they cannot be differentiated from malaria-related effects. METHODS: The effects on renal function of an acute infusion of sodium artesunate (12 mg/kg body weight) were studied in the rat with clearance techniques. We also evaluate the effect of sodium artesunate on chloride lumen-bath flux (Cl Jlb) in the isolated thick ascending limb of the loop of Henle (TALH) microperfused in vitro.

Campos, S. B.
Rouch, L. H.
Seguro, A. C.
Publication Title: 

The spleen plays a central role in the pathophysiology of several potentially severe diseases such as inherited red cell membrane disorders, hemolytic anemias, and malaria. Research on these diseases is hampered by ethical constraints that limit human spleen tissue explorations. We identified a surgical situation--left splenopancreatectomy for benign pancreas tumors--allowing spleen retrieval at no risk for patients. Ex vivo perfusion of retrieved intact spleens for 4 to 6 hours maintained a preserved parenchymal structure, vascular flow, and metabolic activity.

Buffet, Pierre A.
Milon, Geneviève
Brousse, Valentine
Correas, Jean-Michel
Dousset, Bertrand
Couvelard, Anne
Kianmanesh, Reza
Farges, Olivier
Sauvanet, Alain
Paye, François
Ungeheuer, Marie-Noëlle
Ottone, Catherine
Khun, Huot
Fiette, Laurence
Guigon, Ghislaine
Huerre, Michel
Mercereau-Puijalon, Odile
David, Peter H.
Publication Title: 
Journal of Ethnopharmacology

Oxidative damage to crucial biomolecules due to excess generation of reactive oxygen species has been implicated as a major cause of organ damage and hence compounds capable of negating such damage have potential benefits. Using hydrogen peroxide (H2O2) as a model pro-oxidant to induce oxidative stress, we have examined the ability of natural food supplement Maharishi Amrit Kalash (MAK-4) to decrease oxidative damage in potassium-arrested isolated rat hearts.

Cullen, W. J.
Dulchavsky, S. A.
Devasagayam, T. P.
Venkataraman, B. V.
Dutta, S.
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

Flavonoids within Scutellaria baicalensis may be potent antioxidants on the basis of our studies of S. baicalensis extract. To further this work, we studied the antioxidative effects of baicalein, a flavonoid component of S. baicalensis, in a chick cardiomyocyte model of reactive oxygen species (ROS) generation during hypoxia, simulated ischemia-reperfusion, or mitochondrial complex III inhibition with antimycin A. Oxidant stress was measured by oxidation of the intracellular probes 2',7'-dichlorofluorescin diacetate and dihydroethidium.

Shao, Zuo-Hui
Vanden Hoek, Terry L.
Qin, Yimin
Becker, Lance B.
Schumacker, Paul T.
Li, Chang-Qing
Dey, Lucy
Barth, Eugene
Halpern, Howard
Rosen, Gerald M.
Yuan, Chun-Su
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

The purpose of present study was to determine the intestinal absorption and metabolism of genistein and its analogs to better understand the mechanisms responsible for their low oral bioavailability. The Caco-2 cell culture model and a perfused rat intestinal model were used for the study. In both models, permeabilities of aglycones (e.g., genistein) were comparable to well absorbed compounds, such as testosterone and propranolol.

Liu, Yan
Hu, Ming
Publication Title: 
Journal of Alternative and Complementary Medicine (New York, N.Y.)

OBJECTIVE: The objective of this study was to determine the intestinal and microbial disposition of flavonoids and how these disposition processes affect their enteric recycling. DESIGN: Studies were performed using a perfused rat intestinal model or using enrichment cultures and a pure isolate of Enterococcus avium (LY1). RESULTS: In the rat intestine, aglycones, such as quercetin and apigenin, were as permeable (P*(eff) > or = 2) as compounds such as propranolol (100% absorption).

Liu, Yong
Liu, Yan
Dai, Yang
Xun, Luying
Hu, Ming


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