Publication Title: 
Scientific Reports

Vascular endothelial growth factor A (VEGFA) plays an important role in tumour angiogenesis and its angiogenic action is mainly mediated through its VEGF receptor 2 (VEGFR-2). Therefore drugs targeting VEGFA/VEGFR-2 are being presently used in the clinics for treatment of several types of solid malignant tumours. We here in report that low dose of chebulagic acid (CA), a hydrolysable tannin found in myrobalan fruits can inhibit VEGFA induced vascular permeability, endothelial cell proliferation, migration, tube formation and thereby, angiogenesis by suppressing VEGFR-2 phosphorylation.

Lu, Kai
Basu, Sujit
Publication Title: 
Journal of Neuro-Oncology

Regardless of their cell type of origin, all aggressive brain tumors, such as malignant gliomas and metastatic tumors produce brain edema, which is an important cause of patient morbidity and mortality. Caloric restriction (CR) has long been recognized as a natural therapy that improves health, promotes longevity, and significantly reduces both the incidence and growth of many tumor types. The aim of present work was to investigate the effect of CR on edema and survival in the mice implanted with U87 gliomas.

Jiang, Yong-Sheng
Wang, Fu-Rong
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

The objective of this study was to investigate whether the decrease in artemisinin bioavailability after repeated oral dosing in humans can be a result of increased efflux of artemisinin by P-glycoprotein or decreased membrane transport at the intestinal barrier. The effective jejunal permeability (Peff) of artemisinin was investigated using an in situ rat perfusion model. Fifty-four rats were randomized to one of three treatment arms: no pretreatment, pretreatment with artemisinin emulsion for 5 days (60 mg/kg/day, p.o. ), or pretreatment with emulsion vehicle for 5 days.

Svensson, U. S.
Sandström, R.
Carlborg, O.
Lennernäs, H.
Ashton, M.
Publication Title: 
The Journal of Biological Chemistry

The antimalarial agent artesunate (ART) activates programmed cell death (PCD) in cancer cells in a manner dependent on the presence of iron and the generation of reactive oxygen species. In malaria parasites, ART cytotoxicity originates from interactions with heme-derived iron within the food vacuole. The analogous digestive compartment of mammalian cells, the lysosome, similarly contains high levels of redox-active iron and in response to specific stimuli can initiate mitochondrial apoptosis.

Hamacher-Brady, Anne
Stein, Henning A.
Turschner, Simon
Toegel, Ina
Mora, Rodrigo
Jennewein, Nina
Efferth, Thomas
Eils, Roland
Brady, Nathan R.
Publication Title: 
Molecules (Basel, Switzerland)

The Density Functional Theory (DFT) method and the 6-31G** basis set were employed to calculate the molecular properties of artemisinin and 20 derivatives with different degrees of cytotoxicity against the human hepatocellular carcinoma HepG2 line. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were employed to select the most important descriptors related to anticancer activity. The significant molecular descriptors related to the compounds with anticancer activity were the ALOGPS_log, Mor29m, IC5 and GAP energy.

Vieira, Josinete B.
Braga, Francinaldo S.
Lobato, Cleison C.
Santos, César F.
Costa, Josivan S.
Bittencourt, José Adolfo H. M.
Brasil, Davi S. B.
Silva, Jocivânia O.
Hage-Melim, Lorane I. S.
Macêdo, Williams Jorge C.
Carvalho, José Carlos T.
Santos, Cleydson Breno R.
Publication Title: 
European Journal of Pharmaceutical Sciences: Official Journal of the European Federation for Pharmaceutical Sciences

Traditionally Boswellia serrata extract is used in the Indian Ayurvedic medicine for the treatment of inflammatory diseases. In 2002 the EMEA designated Boswellia an orphan drug status for the treatment of peritumoral oedema. Pharmacokinetic studies yielded low plasma concentrations of the active ingredients 11-keto-beta-boswellic acid (KBA) and 3-acetyl-11-keto-beta-boswellic acid (AKBA). In continuation of the tests investigating the factors limiting bioavailability of boswellic acids, the present study examined the permeability of KBA and AKBA in human Caco-2 cell lines.

Krüger, Phillip
Kanzer, Johanna
Hummel, Jessica
Fricker, Gert
Schubert-Zsilavecz, Manfred
Abdel-Tawab, Mona
Publication Title: 
Alcoholism, Clinical and Experimental Research

BACKGROUND: Clinical and animal data indicate that gut-derived endotoxin and other luminal bacterial products are necessary cofactors for development of alcoholic liver disease (ALD). Although gut leakiness is clearly an important cause of endotoxemia in ALD, it cannot fully explain endotoxemia in all ALD subjects and thus other factors may be involved. One possible factor is a change in gut microbiota composition (dysbiosis).

Mutlu, Ece
Keshavarzian, Ali
Engen, Phillip
Forsyth, Christopher B.
Sikaroodi, Masoumeh
Gillevet, Patrick
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis

The purpose of this research was to develop a sensitive and reproducible UPLC-MS/MS method to analyze matrine, an anticancer compound, and to use it to investigate its biopharmaceutical and pharmacokinetic behaviors in rats. A sensitive and fast UPLC-MS/MS method was successfully applied to determine matrine in rat plasma, intestinal perfusate, bile, microsomes, and cell incubation media. The absolute oral bioavailability of matrine is 17.1+/-5.4% at a dose of 2mg/kg matrine.

Yang, Zhen
Gao, Song
Yin, Taijun
Kulkarni, Kaustubh H.
Teng, Yang
You, Ming
Hu, Ming
Publication Title: 
Physiological Reviews

The primary functions of the gastrointestinal tract have traditionally been perceived to be limited to the digestion and absorption of nutrients and to electrolytes and water homeostasis. A more attentive analysis of the anatomic and functional arrangement of the gastrointestinal tract, however, suggests that another extremely important function of this organ is its ability to regulate the trafficking of macromolecules between the environment and the host through a barrier mechanism.

Fasano, Alessio
Publication Title: 
Pediatric Research

Preterm infants face many challenges in transitioning from the in utero to extrauterine environment while still immature. Failure of the preterm gut to successfully mature to accommodate bacteria and food substrate leads to significant morbidity such as neonatal necrotizing enterocolitis. The intestinal epithelial barrier plays a critical role in gut protection. Heat shock protein 70 (Hsp70) is an inducible cytoprotective molecule shown to protect the intestinal epithelium in adult models.

Liedel, Jennifer L.
Guo, Yuee
Yu, Yueyue
Shiou, Sheng-Ru
Chen, Sangzi
Petrof, Elaine O.
Hu, Shien
Musch, Mark W.
Claud, Erika C.


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