Polymorphism, Restriction Fragment Length

Publication Title: 
Acta Virologica

Leaf tissues of stone fruit trees (plum, apricot, peach and myrobalan) carrying symptoms of plum pox virus (PPV) infection and of peach GF 305 seedlings and Nicotiana benthamiana infected experimentally with PPV were assayed for PPV by polymerase chain reaction (PCR). The expected 243 bp PCR products were subjected to restriction fragment length polymorphism (RFLP) analysis with restriction endonucleases AluI and RsaI. All of the PCR products contained the AluI site.

Kúdela, O.
Glasa, M.
Fuchs, E.
Kúdelová, M.
Publication Title: 
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Prunus species express different ranges and levels of resistance to the root-knot nematodes (RKN) Meloidogyne spp. In Myrobalan plum ( Prunus cerasifera), the dominant Ma gene confers a high-level and wide-spectrum resistance to the predominant RKN, Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica and the isolate Meloidogyne sp. Florida which overcomes the resistance of the Amygdalus sources. In Japanese plum ( Prunus salicina), a similar wide-spectrum dominant resistance gene, termed R(jap), has been hypothesized from an intraspecific segregating cross.

Claverie, M.
Bosselut, N.
Lecouls, A. C.
Voisin, R.
Lafargue, B.
Poizat, C.
Kleinhentz, M.
Laigret, F.
Dirlewanger, E.
Esmenjaud, D.
Publication Title: 
Biological & Pharmaceutical Bulletin

Terminalia, a large genus of Combretaceae, is distributed in Tropical Asia, Africa, and America. Some Terminalia plants are used in folk medicine because they possess powerful medicinal properties. Dried fruits of Terminalia bellirica and Terminalia chebula are used as the main ingredient in Triphala, a famous polyherbal formulation in Ayurvedic medicine and Thai folk medicine, because of their laxative, detoxifying, and rejuvenating effects. To clarify the phylogenetic relationships of medicinal Terminalia species (T. bellirica, T. chebula, and T.

Intharuksa, Aekkhaluck
Ando, Hirokazu
Miyake, Katsunori
Sirisa-Ard, Panee
Mikage, Masayuki
Sasaki, Yohei
Publication Title: 

Cell cycle checkpoints and tumor suppressor gene functions appear to be required for the maintenance of a stable genome in proliferating cells. In this study chromosomal destabilization was monitored in relation to telomere structure, lifespan control and G2 checkpoint function. Replicative senescence was inactivated in secondary cultures of human skin fibroblasts by expressing the human papillomavirus type 16 (HPV-16) E6 oncoprotein to inactivate p53. Chromosome aberrations were enumerated during in vitro aging of isogenic control (F5neo) and HPV-16E6-expressing (F5E6) fibroblasts.

Filatov, L.
Golubovskaya, V.
Hurt, J. C.
Byrd, L. L.
Phillips, J. M.
Kaufmann, W. K.
Publication Title: 

Despite great interest in the role of lipids in overall and disease-free survival, virtually no information is available on the lipids, lipoproteins and apolipoproteins of persons over 90 years of age. Furthermore, the genetic underpinnings of atherosclerosis and the particular genetic factors responsible for protection against coronary artery disease remain speculative.

Thieszen, S. L.
Hixson, J. E.
Nagengast, D. J.
Wilson, J. E.
McManus, B. M.
Publication Title: 
Tropical medicine & international health: TM & IH

OBJECTIVE: To test the efficacy of a new compound drug (CGP 56697) against acute, uncomplicated falciparum malaria. METHOD: Reappearing parasites were analysed by PCR-RFLP within a randomized controlled trial. 130 patients received chloroquine and 130 patients were treated with CGP 56697. Samples from 96 patients with parasitological failure were tested by PCR-RFLP for MSP2 of Plasmodium falciparum. Seven days after treatment 32 patients of the chloroquine control group with reappearing parasites were tested by PCR and one infection was unequivocally determined as a new infection.

Irion, A.
Felger, I.
Abdulla, S.
Smith, T.
Mull, R.
Tanner, M.
Hatz, C.
Beck, H. P.
Publication Title: 
Tropical medicine & international health: TM & IH

OBJECTIVE: Artemether-lumefantrine (AL), presently the most favoured combination therapy against uncomplicated Plasmodium falciparum malaria in Africa, has recently shown to select for the pfmdr1 86N allele. The objective of this study was to search for the selection of other mutations potentially involved in artemether-lumefantrine tolerance and/or resistance, i.e. pfmdr1 gene amplification, pfmdr1 Y184F, S1034C, N1042D, D1246Y, pfcrt S163R and PfATP6 S769N.

Sisowath, Christin
Ferreira, Pedro E.
Bustamante, Leyla Y.
Dahlstrom, Sabina
Mårtensson, Andreas
Björkman, Anders
Krishna, Sanjeev
Gil, José P.
Publication Title: 
Malaria Journal

BACKGROUND: The emergence of Plasmodium falciparum resistant to most currently used antimalarial drugs is the major problem in malaria control along the Thai-Myanmar and Thai-Cambodia borders. Although artemisinin-based combination therapy has been recommended for the treatment of multidrug-resistant falciparum malaria, these combinations are not available for some people, such as travelers from North America.

Khositnithikul, Rommanee
Tan-Ariya, Peerapan
Mungthin, Mathirut
Publication Title: 
Tropical medicine & international health: TM & IH

OBJECTIVE: To evaluate the in vitro efficacy of artesunate (ATN) and artemether (ATH) against Plasmodium falciparum isolates from the Brazilian Amazon state of Pará and to search for mutations and/or altered copy numbers in the putative resistance-associated pfcrt, pfmdr1 and pfATPase6 genes. METHODS: In vitro efficacy of ATN and ATH was successfully measured in 56 freshly collected P. falciparum isolates, using a conventional WHO microtest with minor modifications. Single nucleotide polymorphisms (SNPs) in the same isolates were inspected using DNA sequencing and/or PCR-RFLP.

Ferreira, Isabel D.
Martinelli, Axel
Rodrigues, Louise A.
do Carmo, Ediclei L.
do Rosario, Virgilio E.
Póvoa, Marinete M.
Cravo, Pedro
Publication Title: 
Malaria Journal

BACKGROUND: The emergence of drug resistance is a major problem in malaria control. For mathematical modelling of the transmission and spread of drug resistance the determinant parameters need to be identified and measured. The underlying hypothesis is that mutations associated with drug resistance incur fitness costs to the parasite in absence of drug pressure. The distribution of drug resistance haplotypes in different subsets of the host population was investigated.

Schoepflin, Sonja
Marfurt, Jutta
Goroti, Mary
Baisor, Moses
Mueller, Ivo
Felger, Ingrid


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