Protease Inhibitors

Publication Title: 
Scientific Reports

The physiological roles of the protease inhibitor SERPINB3 (SB3) are still largely unknown. The study was addressed to assess the biological effects of this serpin in vivo using a SB3 transgenic mouse model. Two colonies of mice (123 transgenic for SB3 and 148 C57BL/6J controls) have been studied. Transgenic (TG) mice showed longer survival than controls and the difference was more remarkable in males than in females (18.5% vs 12.7% life span increase). In TG mice decreased IL-6 in serum and lower p66shc in the liver were observed.

Villano, Gianmarco
Ruvoletto, Mariagrazia
Ceolotto, Giulio
Quarta, Santina
Calabrese, Fiorella
Turato, Cristian
Tono, Natascia
Biasiolo, Alessandra
Cattelan, Arianna
Merkel, Carlo
Avogaro, Angelo
Gatta, Angelo
Pontisso, Patrizia
Publication Title: 
FEMS microbiology letters

Interactions between antimicrobial agents provide clues as to their mechanisms of action and influence the combinations chosen for therapy of infectious diseases. In the treatment of malaria, combinations of drugs, in many cases acting synergistically, are increasingly important in view of the frequency of resistance to single agents. The study of antimalarial drug interactions is therefore of great significance to both treatment and research.

Bell, Angus
Publication Title: 
Journal of Periodontology

BACKGROUND: This study evaluated the inhibitory activity of triphala on PMN-type matrix metalloproteinase (MMP-9) expressed in adult periodontitis patients and compared its activity with another ayurvedic drug, kamillosan, and doxycycline, which has known inhibitory activity. METHODS: Matrix metalloproteinases (MMPs) were extracted from gingival tissue samples from 10 patients (six males, four females) with chronic periodontitis.

Abraham, Sajith
Kumar, M. Senthil
Sehgal, P. K.
Nitish, S.
Jayakumar, N. D.
Publication Title: 

Insulin-like growth factor binding protein-3 (IGFBP-3) can cause growth suppressive and proapoptotic effects on retinoids in many types of cancer cells. However, the expression and effects of IGFBP-3 in myeloid leukemia cells have not been elucidated. In this study, we found no IGFBP-3 expression in the human myeloid leukemia cell lines either at baseline or after stimulation with all-trans retinoic acid (ATRA). Human recombinant IGFBP-3 induced growth arrest and apoptosis of HL-60 and NB4 cells.

Ikezoe, Takayuki
Tanosaki, Sakae
Krug, Utz
Liu, Bingrong
Cohen, Pinchas
Taguchi, Hirokuni
Koeffler, H. Phillip
Publication Title: 
Molecular Vision

PURPOSE: The purpose was to characterize the properties of a proteinase activity associated with betaA3-crystallin, which was isolated from the alpha-crystallin fraction of human lenses. METHODS: An inactive, Arg-bond hydrolyzing proteinase in the alpha-crystallin fraction, which was isolated from the water soluble (WS) protein fraction of 60- to 70-year-old human lenses, was activated by sodium deoxycholate treatment.

Srivastava, O. P.
Srivastava, K.
Chaves, J. M.
Publication Title: 
Journal of the National Medical Association

BACKGROUND/OBJECTIVE: HIV infection has a devastating impact on individual and public health, and affects populations disproportionately. Treatment with antiretroviral therapy (ART) saves lives, but long-term adherence to ART is critical to its success. We performed an observational cohort study to determine the influence of race, sex and other sociodemographic factors on early ART discontinuations among HIV-infected persons.

Asad, Shaheena
Hulgan, Todd
Raffanti, Stephen P.
Daugherty, Jim
Ray, Wayne
Sterling, Timothy R.
Publication Title: 
American Journal of Physiology. Endocrinology and Metabolism

Inclusion of HIV protease inhibitors (PIs) in the treatment of people living with HIV+ has markedly decreased mortality but also increased the incidence of metabolic abnormalities, causes of which are not well understood. Here, we report that insulinopenia is exacerbated when Zucker fa/fa rats are exposed to a PI for 7 wk, suggesting that chronic PI exposure adversely affects pancreatic islet beta-cell function.

Zhang, Sheng
Carper, Michael J.
Lei, Xiaoyong
Cade, W. Todd
Yarasheski, Kevin E.
Ramanadham, Sasanka
Publication Title: 
Arthritis Research & Therapy

INTRODUCTION: The major risk factor for osteoarthritis (OA) is aging, but the mechanisms underlying this risk are only partly understood. Age-related accumulation of advanced glycation end products (AGEs) can activate chondrocytes and induce the production of proinflammatory cytokines and matrix metalloproteinases (MMPs). In the present study, we examined the effect of epigallocatechin-3-gallate (EGCG) on AGE-modified-BSA (AGE-BSA)-induced activation and production of TNFalpha and MMP-13 in human OA chondrocytes.

Rasheed, Zafar
Anbazhagan, Arivarasu N.
Akhtar, Nahid
Ramamurthy, Sangeetha
Voss, Frank R.
Haqqi, Tariq M.
Publication Title: 
Respiratory Research

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a common respiratory disorder for which new diagnostic and therapeutic approaches are required. Hallmarks of COPD are matrix destruction and neutrophilic airway inflammation in the lung. We have previously described two tri-peptides, N-alpha-PGP and PGP, which are collagen fragments and neutrophil chemoattractants. In this study, we investigate if N-alpha-PGP and PGP are biomarkers and potential therapeutic targets for COPD.

O'Reilly, Philip
Jackson, Patricia L.
Noerager, Brett
Parker, Suzanne
Dransfield, Mark
Gaggar, Amit
Blalock, J. Edwin
Publication Title: 
International Journal of Obesity (2005)

OBJECTIVE: To investigate the mechanisms underlying the satiety-promoting effects of a novel protease inhibitor concentrate derived from potato (PPIC). METHODS: The acute and prolonged effects of oral PPIC administration (100 mg kg(-1) per day) on food intake, body weight and gastric emptying were evaluated in healthy rats. Parameters of body weight, food intake, plasma glucose, insulin and cholecystokinin (CCK) were measured. Duodenal proteolytic activity and CCK expression were determined in tissue extracts.

Komarnytsky, S.
Cook, A.
Raskin, I.


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