Protein Denaturation

Publication Title: 
Biochimica Et Biophysica Acta

Botulinum neurotoxins (BoNTs) are proteins of great interest not only because of their extreme toxicity but also paradoxically for their therapeutic applications. All the known serotypes (A-G) have varying degrees of longevity and potency inside the neuronal cell. Differential chemical modifications such as phosphorylation and ubiquitination have been suggested as possible mechanisms for their longevity, but the molecular basis of the longevity remains unclear.

Kumar, Raj
Kukreja, Roshan V.
Cai, Shuowei
Singh, Bal R.
Publication Title: 
Biochemical Pharmacology

HIV protease inhibitor (PI)-associated cardiovascular risk, especially atherosclerosis, has become a major concern in the clinic. Macrophages are key players in the inflammatory response and atherosclerosis formation. We have previously shown that HIV PIs induce endoplasmic reticulum (ER) stress, activate the unfolded protein response (UPR), and increase the synthesis of the inflammatory cytokines, TNF-alpha and IL-6, by regulating the intracellular translocation of RNA binding protein HuR in macrophages. However, the underlying signaling mechanisms remain unclear.

Chen, Li
Jarujaron, Sirikalaya
Wu, Xudong
Sun, Lixin
Zha, Weibin
Liang, Guang
Wang, Xuan
Gurley, Emily C.
Studer, Elaine J.
Hylemon, Phillip B.
Pandak, William M.
Zhang, Luyong
Wang, Guangji
Li, Xiaokun
Dent, Paul
Zhou, Huiping
Publication Title: 
Toxicological Sciences: An Official Journal of the Society of Toxicology

An animal model for food allergy is needed to assess genetically modified food crops for potential allergenicity. The ideal model must produce allergic antibody (IgE) to proteins differentially according to known allergenicity before being used to accurately identify potential allergens among novel proteins. The oral route is the most relevant for exposure to food antigens, and a protein's stability to digestion is a current risk assessment tool based on this natural route.

Bowman, Christal C.
Selgrade, Maryjane K.
Publication Title: 
European Journal of Endocrinology / European Federation of Endocrine Societies

OBJECTIVE: Decreased levels of GH and total IGF-I have been reported in obesity. It has been hypothesized that increased free (biologically active) IGF-I levels generated from IGF-binding protein (IGFBP) protease activity could be the mechanism for the low GH release in dieting obese subjects. However, no published data exist on free IGF-I levels, acid labile subunit (ALS), or IGFBP protease activity in relation to GH release during a hypocaloric diet.

Rasmussen, Michael Højby
Juul, Anders
Kjems, Lise Lund
Hilsted, Jannik
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