Proto-Oncogene Proteins c-mdm2

Publication Title: 
Free Radical Biology & Medicine

Apigenin, a plant flavone, potentially activates wild-type p53 and induces apoptosis in cancer cells. We conducted detailed studies to understand its mechanism of action. Exposure of human prostate cancer 22Rv1 cells, harboring wild-type p53, to growth-suppressive concentrations (10-80 microM) of apigenin resulted in the stabilization of p53 by phosphorylation on critical serine sites, p14ARF-mediated downregulation of MDM2 protein, inhibition of NF-kappaB/p65 transcriptional activity, and induction of p21/WAF-1 in a dose- and time-dependent manner.

Author(s): 
Shukla, Sanjeev
Gupta, Sanjay
Publication Title: 
American Journal of Physiology. Gastrointestinal and Liver Physiology

The inflammatory response during pancreatitis regulates necrotic and apoptotic rates of parenchymal cells. Neutrophil depletion by use of anti-polymorphonuclear serum (anti-PMN) increases apoptosis in experimental pancreatitis but the mechanism has not been determined. Our study was designed to investigate signaling mechanisms in pancreatic parenchymal cells regulating death responses with neutrophil depletion. Rats were neutrophil depleted with anti-PMN treatment. Then cerulein pancreatitis was induced, followed by measurements of apoptosis signaling pathways.

Author(s): 
Nakamura, Yuji
Do, Jae Hyuk
Yuan, Jingzhen
Odinokova, Irina V.
Mareninova, Olga
Gukovskaya, Anna S.
Pandol, Stephen J.
Publication Title: 
Arthritis and Rheumatism

OBJECTIVE: The protein deacetylase SirT1 inhibits apoptosis in a variety of cell systems by distinct mechanisms, yet its role in chondrocyte death has not been explored. We undertook the present study to assess the role of SirT1 in the survival of osteoarthritic (OA) chondrocytes in humans. METHODS: SirT1, protein tyrosine phosphatase 1B (PTP1B), and PTP1B mutant expression plasmids as well as SirT1 small interfering RNA (siRNA) and PTP1B siRNA were transfected into primary human chondrocytes.

Author(s): 
Gagarina, Viktoria
Gabay, Odile
Dvir-Ginzberg, Mona
Lee, Eun Jin
Brady, Jillian K.
Quon, Michael J.
Hall, David J.
Publication Title: 
Hepatology (Baltimore, Md.)

Hu antigen R (HuR) is a central RNA-binding protein regulating cell dedifferentiation, proliferation, and survival, which are well-established hallmarks of cancer. HuR is frequently overexpressed in tumors correlating with tumor malignancy, which is in line with a role for HuR in tumorigenesis. However, the precise mechanism leading to changes in HuR expression remains unclear. In the liver, HuR plays a crucial role in hepatocyte proliferation, differentiation, and transformation.

Author(s): 
Embade, Nieves
Fernández-Ramos, David
Varela-Rey, Marta
Beraza, Naiara
Sini, Marcella
Gutiérrez de Juan, Virginia
Woodhoo, Ashwin
Martínez-López, Nuria
Rodríguez-Iruretagoyena, Begoña
Bustamante, Francisco Javier
de la Hoz, Ana Belén
Carracedo, Arkaitz
Xirodimas, Dimitris P.
Rodríguez, Manuel S.
Lu, Shelly C.
Mato, José M.
Martínez-Chantar, María L.
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