Rats, Inbred BN

Publication Title: 
Experimental Gerontology

Chronic caloric restriction (CR) has been demonstrated to increase longevity in lower species and studies are ongoing to evaluate its effect in higher species. A consistent metabolic feature of CR is improved insulin sensitivity and lowered lifetime glycemia, yet the mechanism responsible is currently unknown. However, the membrane's physiochemical properties, as determined by phospholipid composition, have been related to insulin action in animal and human studies and CR has been reported to alter membrane lipid content.

Author(s): 
Cefalu, W. T.
Wang, Z. Q.
Bell-Farrow, A. D.
Terry, J. G.
Sonntag, W.
Waite, M.
Parks, J.
Publication Title: 
Alcohol (Fayetteville, N.Y.)

Male Fischer 344 (F-344) rats of three different ages (4, 13, and 25 months) and male Brown Norway (BN/BIRIJ) rats (4 months) were injected (IP) with a hypnotic dose of ethanol (3.0 g/kg, 10% w/v in saline). Half of the animals were decapitated and brains extracted immediately upon loss of the righting reflex (LRR), while the other half were allowed to reach recovery of the righting reflex (RRR), at which time brains were extracted.

Author(s): 
York, J. L.
Chan, A. W.
Publication Title: 
The Journal of Neuroscience: The Official Journal of the Society for Neuroscience

Huntington's disease (HD) is a fully penetrant autosomal-dominant inherited neurological disorder caused by expanded CAG repeats in the Huntingtin gene. Transcriptional dysfunction, excitotoxicity, and oxidative stress have all been proposed to play important roles in the pathogenesis of HD. This study was designed to explore the therapeutic potential of mithramycin, a clinically approved guanosine-cytosine-rich DNA binding antitumor antibiotic.

Author(s): 
Ferrante, Robert J.
Ryu, Hoon
Kubilus, James K.
D'Mello, Santosh
Sugars, Katharine L.
Lee, Junghee
Lu, Peiyuan
Smith, Karen
Browne, Susan
Beal, M. Flint
Kristal, Bruce S.
Stavrovskaya, Irina G.
Hewett, Sandra
Rubinsztein, David C.
Langley, Brett
Ratan, Rajiv R.
Publication Title: 
British Journal of Pharmacology

BACKGROUND AND PURPOSE: The age-related decline in vasorelaxation is largely due to ceramide-induced induction of phosphatase 2A (PP2A), which limits nitric oxide synthase (eNOS) phosphorylation at stimulatory sites. We hypothesized that ceramide accumulation was from an age-related loss of endothelial glutathione (GSH) and subsequent activation of neutral sphingomyelinase (nSMase), an enzyme whose activity increases when GSH is limited. EXPERIMENTAL APPROACH: Old (30-32 mo) F344xBN rats were given (R)-alpha-lipoic acid (LA), an agent known to induce GSH synthesis.

Author(s): 
Smith, A. R.
Visioli, F.
Frei, B.
Hagen, T. M.
Publication Title: 
PloS One

BACKGROUND: Mitochondrial dysfunction and oxidative stress are central mechanisms underlying the aging process and the pathogenesis of many age-related diseases. Selected antioxidants and specific combinations of nutritional compounds could target many biochemical pathways that affect both oxidative stress and mitochondrial function and, thereby, preserve or enhance physical performance.

Author(s): 
Xu, Jinze
Seo, Arnold Y.
Vorobyeva, Darya A.
Carter, Christy S.
Anton, Stephen D.
Lezza, Angela M. S.
Leeuwenburgh, Christiaan
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

There is strong evidence showing that aging is associated with vascular oxidative stress, which has been causally linked to the development of cardiovascular diseases. NF-E2-related factor-2 (Nrf2) is a transcription factor, which is activated by reactive oxygen species in the vasculature of young animals leading to the upregulation of various antioxidant genes. The present study was designed to elucidate age-related changes in the homeostatic role of Nrf2-driven free radical detoxification mechanisms in the vasculature.

Author(s): 
Ungvari, Zoltan
Bailey-Downs, Lora
Sosnowska, Danuta
Gautam, Tripti
Koncz, Peter
Losonczy, Gyorgy
Ballabh, Praveen
de Cabo, Rafael
Sonntag, William E.
Csiszar, Anna
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

The elderly patients show a significantly elevated mortality rate during sepsis than younger patients, due to their higher propensity to microvascular dysfunction and consequential multiorgan failure. We tested whether aging renders vascular endothelial cells more susceptible to damage induced by inflammatory factors present in the circulation during sepsis. Primary microvascular endothelial cells derived from young (3 months) and aged (24 months) Fischer 344 × Brown Norway rats were treated with sera obtained from sepsis patients and healthy controls.

Author(s): 
Tucsek, Zsuzsanna
Gautam, Tripti
Sonntag, William E.
Toth, Peter
Saito, Hiroshi
Salomao, Reinaldo
Szabo, Csaba
Csiszar, Anna
Ungvari, Zoltan
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

Age-related impairment of angiogenesis is likely to play a central role in cerebromicrovascular rarefaction and development of vascular cognitive impairment, but the underlying mechanisms remain elusive. To test the hypothesis that dysregulation of Dicer1 (ribonuclease III, a key enzyme of the microRNA [miRNA] machinery) impairs endothelial angiogenic capacity in aging, primary cerebromicrovascular endothelial cells (CMVECs) were isolated from young (3 months old) and aged (24 months old) Fischer 344 × Brown Norway rats.

Author(s): 
Ungvari, Zoltan
Tucsek, Zsuzsanna
Sosnowska, Danuta
Toth, Peter
Gautam, Tripti
Podlutsky, Andrej
Csiszar, Agnes
Losonczy, Gyorgy
Valcarcel-Ares, M. Noa
Sonntag, William E.
Csiszar, Anna
Publication Title: 
World journal of gastroenterology: WJG

AIM: To develop a Brown Norway (BN) rat model to determine the potential allergenicity of novel proteins in genetically modified food. METHODS: The allergenicity of different proteins were compared, including ovalbumin (OVA), a potent respiratory and food allergen, bovine serum albumin (BSA), a protein that is considered to have a lesser allergenic potential, and potato acid phosphatase (PAP), a non-allergenic protein when administered to BN rats via different routes of exposure (intraperitoneally or by gavage). IgG and IgE antibody responses were determined by ELISA and PCA, respectively.

Author(s): 
Jia, Xu-Dong
Li, Ning
Wu, Yong-Ning
Yang, Xiao-Guang
Publication Title: 
Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz

The placing on the European Union's market of genetically modified crops requires authorization by the European Commission which is based on the proof that the derived foods are as safe as their conventional counterparts. The assessment of potential allergenicity is part of the necessary investigations recommended in the updated Guidance Document of the Scientific Panel on Genetically Modified Organisms (GMO) of the European Food Safety Authority (EFSA), which is based on internationally agreed recommendations.

Author(s): 
Schauzu, M.
Pöting, A.
Rubin, D.
Lampen, A.

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