Real-Time Polymerase Chain Reaction

Publication Title: 
Nephrology, Dialysis, Transplantation: Official Publication of the European Dialysis and Transplant Association - European Renal Association

BACKGROUND: The bioartificial renal tubule device is a cell therapy system for renal failure. The major obstacle in the development of the bioartificial renal tubule device is the obtainment of a large number of viable renal tubule cells to seed on the inner surface of hollow fibers. Although our previous studies had used a transformed cell line, they may be dangerous for clinical uses. Therefore, different approaches to amplify renal proximal tubular epithelial cells (RPTEC) in culture without oncogenes, vectors and carcinogens have been required.

Author(s): 
Sanechika, Noriyuki
Sawada, Kaichiro
Usui, Yukio
Hanai, Kazuya
Kakuta, Takatoshi
Suzuki, Hajime
Kanai, Genta
Fujimura, Satoshi
Yokoyama, Tun Aung
Fukagawa, Masafumi
Terachi, Toshiro
Saito, Akira
Publication Title: 
Cellular and molecular life sciences: CMLS

Cell adhesion molecule 1 (CADM1), expressed by human lung mast cells (HLMCs), mediates their adhesion to airway smooth muscle (ASM), and contributes to ASM-dependent HLMC proliferation and survival. CADM1 is expressed in alternatively spliced isoforms, but those present in HLMCs and their function are not known. We cloned three functional and one cryptic non-functional isoform with alternative splicing between exons 7/11 and 1/2, respectively, from HLMCs and human MC lines (HMC-1 and LAD2).

Author(s): 
Moiseeva, Elena P.
Leyland, Mark L.
Bradding, Peter
Publication Title: 
Age (Dordrecht, Netherlands)

Throughout life, there is an aging of the immune system that causes impairment of its defense capability. Prevention or delay of this deterioration is considered crucial to maintain general health and increase longevity. We evaluated whether dietary supplementation with Lactobacillus delbrueckii subsp. bulgaricus 8481 could enhance the immune response in the elderly. This multi-center, double-blind, and placebo controlled study enrolled 61 elderly volunteers who were randomly assigned to receive either placebo or probiotics. Each capsule of probiotics contained at least 3?◊?10(7)? L.

Author(s): 
Moro-GarcÌa, Marco Antonio
Alonso-Arias, Rebeca
Baltadjieva, Maria
Fern·ndez BenÌtez, Carlos
Fern·ndez Barrial, Manuel Amadeo
DÌaz Ruis·nchez, Enrique
Alonso Santos, Ricardo
Alvarez S·nchez, Magdalena
Saavedra Mij·n, Juan
LÛpez-Larrea, Carlos
Publication Title: 
Age (Dordrecht, Netherlands)

Curcumin is a polyphenolic bioactive compound in turmeric. We examined if antioxidant effects of curcumin are associated with lifespan extension in Drosophila. In this experiment, females and males of Drosophila were fed diets either containing no curcumin (C0) or supplemented with curcumin at 0.5 (C1) and 1.0 (C2) mg/g of diet. The levels of malondialdehyde (MDA), enzyme activity of superoxide dismutase (SOD), and expression of seven age-related genes in females and males were analyzed.

Author(s): 
Shen, Li-Rong
Xiao, Fa
Yuan, Peng
Chen, Ying
Gao, Qi-Kang
Parnell, Laurence D.
Meydani, Mohsen
Ordovas, Jose M.
Li, Duo
Lai, Chao-Qiang
Publication Title: 
Age (Dordrecht, Netherlands)

Nutraceuticals are known to have numerous health and disease preventing properties. Recent studies suggest that extracts containing cranberry may have anti-aging benefits. However, little is known about whether and how cranberry by itself promotes longevity and healthspan in any organism. Here we examined the effect of a cranberry only extract on lifespan and healthspan in Caenorhabditis elegans. Supplementation of the diet with cranberry extract (CBE) increased the lifespan in C. elegans in a concentration-dependent manner. Cranberry also increased tolerance of C.

Author(s): 
Guha, Sujay
Cao, Min
Kane, Ryan M.
Savino, Anthony M.
Zou, Sige
Dong, Yuqing
Publication Title: 
American Journal of Physiology. Gastrointestinal and Liver Physiology

Aquaporin 11 (AQP11) is a protein channel expressed intracellularly in multiple organs, yet its physiological function is unclear. Aqp11 knockout (KO) mice die early due to malfunction of the kidney, a result of hydropic degeneration of proximal tubule cells. Here we report the generation of liver-specific Aqp11 KO mice, allowing us to study the role of AQP11 protein in liver of mice with normal kidney function. The unchallenged liver-specific Aqp11 KO mice have normal longevity, their livers appeared normal, and the plasma biochemistries revealed only a minor defect in lipid handling.

Author(s): 
Rojek, Aleksandra
F¸chtbauer, Ernst-Martin
F¸chtbauer, Annette
Jelen, Sabina
Malmendal, Anders
Fenton, Robert A.
Nielsen, S¯ren
Publication Title: 
American Journal of Physiology. Endocrinology and Metabolism

Ectopic expression of uncoupling protein 1 (UCP1) in skeletal muscle (SM) mitochondria increases lifespan considerably in high-fat diet-fed UCP1 Tg mice compared with wild types (WT). To clarify the underlying mechanisms, we investigated substrate metabolism as well as oxidative stress damage and antioxidant defense in SM of low-fat- and high-fat-fed mice. Tg mice showed an increased protein expression of phosphorylated AMP-activated protein kinase, markers of lipid turnover (p-ACC, FAT/CD36), and an increased SM ex vivo fatty acid oxidation.

Author(s): 
Keipert, S.
Ost, M.
Chadt, A.
Voigt, A.
Ayala, V.
Portero-Otin, M.
Pamplona, R.
Al-Hasani, H.
Klaus, S.
Publication Title: 
Archives of Gerontology and Geriatrics

Lutein is one of the major carotenoids in most fruits and vegetables. The effect of lutein on the lifespan of Drosophila melanogaster was investigated. Results revealed that 0.1mg lutein/ml diet could prolong their mean lifespan from 49.0 to 54.6 days. This was consistent with a significant reduction in malonyldialdehyde (MDA) level and increase in antioxidant enzyme activities of the flies fed with lutein-treated diet compared with those fed with basal diet. Paraquat (PQ) and H2O2 treatment tests demonstrated that lutein could prolong the survival time of the flies.

Author(s): 
Zhang, Zesheng
Han, Shunkai
Wang, Hao
Wang, Tingting
Publication Title: 
Psychosomatic Medicine

OBJECTIVE: To examine associations between autonomic nervous system and adrenocortical reactivity to laboratory stressors and buccal cell telomere length (BTL) in children. METHODS: The study sample comprised 78 children, aged 5 to 6 years, from a longitudinal cohort study of kindergarten social hierarchies, biologic responses to adversity, and child health. Buccal cell samples and reactivity measures were collected in the spring of the kindergarten year. BTL was measured by real-time polymerase chain reaction, as the telomere-to-single-copy gene ratio.

Author(s): 
Kroenke, Candyce H.
Epel, Elissa
Adler, Nancy
Bush, Nicole R.
Obradovic, Jelena
Lin, Jue
Blackburn, Elizabeth
Stamperdahl, Juliet Lise
Boyce, W. Thomas
Publication Title: 
Psychosomatic Medicine

OBJECTIVE: To examine associations between autonomic nervous system and adrenocortical reactivity to laboratory stressors and buccal cell telomere length (BTL) in children. METHODS: The study sample comprised 78 children, aged 5 to 6 years, from a longitudinal cohort study of kindergarten social hierarchies, biologic responses to adversity, and child health. Buccal cell samples and reactivity measures were collected in the spring of the kindergarten year. BTL was measured by real-time polymerase chain reaction, as the telomere-to-single-copy gene ratio.

Author(s): 
Kroenke, Candyce H.
Epel, Elissa
Adler, Nancy
Bush, Nicole R.
Obradovic, Jelena
Lin, Jue
Blackburn, Elizabeth
Stamperdahl, Juliet Lise
Boyce, W. Thomas

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