Recombinant Fusion Proteins

Publication Title: 
Combinatorial Chemistry & High Throughput Screening

Symptoms associated with menopause can greatly affect the quality of life for women. Botanical dietary supplements have been viewed by the public as safe and effective despite a lack of evidence indicating a urgent necessity to standardize these supplements chemically and biologically.

Author(s): 
Overk, Cassia R.
Yao, Ping
Chen, Shaonong
Deng, Shixing
Imai, Ayano
Main, Matthew
Schinkovitz, Andreas
Farnsworth, Norman R.
Pauli, Guido F.
Bolton, Judy L.
Publication Title: 
Experimental Cell Research

The ceramide synthase (CerS) enzymes are key regulators of ceramide homeostasis. CerS1 is central to regulating C18 ceramide which has been shown to be important in cancer and the response to chemotherapeutic drugs. Previous work indicated that some drugs induced a novel and specific translocation of CerS1 from the endoplasmic reticulum to the Golgi apparatus. We now show that diverse stresses such as UV light, DTT, as well as drugs with different mechanisms of action induce CerS1 translocation.

Author(s): 
Sridevi, Priya
Alexander, Hannah
Laviad, Elad L.
Min, Junxia
Mesika, Adi
Hannink, Mark
Futerman, Anthony H.
Alexander, Stephen
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

Galectin-1 (Gal-1), a β-galactoside-binding protein, can alter fate and effector function of Th cells; however, little is known about how Gal-1 induces Th cell differentiation. In this article, we show that both uncommitted and polarized Th cells bound by Gal-1 expressed an immunoregulatory signature defined by IL-10. IL-10 synthesis was stimulated by direct Gal-1 engagement to cell surface glycoproteins, principally CD45, on activated Th cells and enhanced by IL-21 expression through the c-Maf/aryl hydrocarbon receptor pathway, independent of APCs.

Author(s): 
Cedeno-Laurent, Filiberto
Opperman, Matthew
Barthel, Steven R.
Kuchroo, Vijay K.
Dimitroff, Charles J.
Publication Title: 
The Journal of Nutritional Biochemistry

Retinoids are essential in the proper establishment and maintenance of immunity. Although retinoids are implicated in immune related processes, their role in immune cell adhesion has not been well established. In this study, the effect of 9-cis-retinoic acid (9-cis-RA) on human hematopoietic cell adhesion was investigated. 9-cis-RA treatment specifically induced cell adhesion of the human immune cell lines HuT-78, NB4, RPMI 8866 and U937. Due to the prominent role of integrin receptors in mediating immune cell adhesion, we sought to evaluate if cell adhesion was integrin-dependent.

Author(s): 
Whelan, Jarrett T.
Chen, Jianming
Miller, Jabin
Morrow, Rebekah L.
Lingo, Joshuah D.
Merrell, Kaitlin
Shaikh, Saame Raza
Bridges, Lance C.
Publication Title: 
Methods in Molecular Biology (Clifton, N.J.)

Peroxisome proliferator-activated receptors (PPARs) consist of three subtypes, each displaying distinctive tissue distribution. In general, the three PPAR subtypes exert overlapping function in transcriptional regulation of lipid metabolism. However, each PPAR subtype possesses distinctive functions in different tissues dependent on their expression abundance, endogenous ligands, and the PPAR coregulators in a specific tissue. Transgenesis is an invaluable technique in defining the in vivo function of a particular gene and its protein.

Author(s): 
Kim, Teayoun
Zhelyabovska, Olga
Liu, Jian
Yang, Qinglin
Publication Title: 
Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology

BACKGROUND AND AIMS: Wnt/β-catenin signaling plays important roles in development and cellular processes. The hallmark of canonical Wnt signaling activation is the stabilization of β-catenin protein in cytoplasm and/or nucleus. The stability of β-catenin is the key to its biological functions and is controlled by the phosphorylation of its amino-terminal degradation domain. Aberrant activation of β-catenin signaling has been implicated in the development of human cancers. It has been recently suggested that GSK3βmay play an essential role in regulating global protein turnover.

Author(s): 
Kong, Yuhan
Zhang, Hongyu
Chen, Xian
Zhang, Wenwen
Zhao, Chen
Wang, Ning
Wu, Ningning
He, Yunfeng
Nan, Guoxin
Zhang, Hongmei
Wen, Sheng
Deng, Fang
Liao, Zhan
Wu, Di
Zhang, Junhui
Qin, Xinyue
Haydon, Rex C.
Luu, Hue H.
He, Tong-Chuan
Zhou, Lan
Publication Title: 
Journal of Visualized Experiments: JoVE

Laboratory scale to industrial scale purification of biomolecules from cell culture supernatants and lysed cell solutions can be accomplished using affinity chromatography. While affinity chromatography using porous protein A agarose beads packed in columns is arguably the most common method of laboratory scale isolation of antibodies and recombinant proteins expressing Fc fragments of IgG, it can be a time consuming and expensive process.

Author(s): 
Burdick, Monica M.
Reynolds, Nathan M.
Martin, Eric W.
Hawes, Jacquelyn V.
Carlson, Grady E.
Cuckler, Chaz M.
Bates, Michael C.
Barthel, Steven R.
Dimitroff, Charles J.
Publication Title: 
The Journal of Biological Chemistry

Activation of NAD-dependent deacetylases, or Sirtuins, prolongs life span and mimics the effects of caloric restriction in yeast. The FoxO subfamily of forkhead transcription factors has been shown to mediate some of the effects of Sirtuins. Here we have shown that Sirtuin activation or hydrogen peroxide treatment overrides the phosphorylation-dependent nuclear exclusion of FoxO1 caused by growth factors and causes nuclear translocation of FoxO1 in hepatocytes.

Author(s): 
Frescas, David
Valenti, Luca
Accili, Domenico
Publication Title: 
Science (New York, N.Y.)

Arsenic, an ancient drug used in traditional Chinese medicine, has attracted worldwide interest because it shows substantial anticancer activity in patients with acute promyelocytic leukemia (APL). Arsenic trioxide (As2O3) exerts its therapeutic effect by promoting degradation of an oncogenic protein that drives the growth of APL cells, PML-RARalpha (a fusion protein containing sequences from the PML zinc finger protein and retinoic acid receptor alpha).

Author(s): 
Zhang, Xiao-Wei
Yan, Xiao-Jing
Zhou, Zi-Ren
Yang, Fei-Fei
Wu, Zi-Yu
Sun, Hong-Bin
Liang, Wen-Xue
Song, Ai-Xin
Lallemand-Breitenbach, Valérie
Jeanne, Marion
Zhang, Qun-Ye
Yang, Huai-Yu
Huang, Qiu-Hua
Zhou, Guang-Biao
Tong, Jian-Hua
Zhang, Yan
Wu, Ji-Hui
Hu, Hong-Yu
de Thé, Hugues
Chen, Sai-Juan
Chen, Zhu
Publication Title: 
Molecular Vision

PURPOSE: The receptor for advanced glycation end products (RAGE) has been implicated in the pathogenesis of numerous complications of diabetes. We assessed the effect of a novel RAGE fusion protein inhibitor on retinal histopathology and nerve function, and on retinal inflammation and oxidative stress. METHODS: C57BL/6J mice were made diabetic with streptozotocin, and some were given a RAGE fusion protein (10, 100, or 300 µg per mouse 3 times per week).

Author(s): 
Li, Guangyuan
Tang, Jie
Du, Yunpeng
Lee, Chieh Allen
Kern, Timothy S.

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