Replication Origin

Publication Title: 
Nucleic Acids Research

The eukaryotic DNA replication initiation factor Mcm10 is essential for both replisome assembly and function. Human Mcm10 has two DNA-binding domains, the conserved internal domain (ID) and the C-terminal domain (CTD), which is specific to metazoans. SIRT1 is a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase that belongs to the sirtuin family. It is conserved from yeast to human and participates in cellular controls of metabolism, longevity, gene expression and genomic stability.

Author(s): 
Fatoba, Samuel T.
Tognetti, Silvia
Berto, Melissa
Leo, Elisabetta
Mulvey, Claire M.
Godovac-Zimmermann, Jasminka
Pommier, Yves
Okorokov, Andrei L.
Publication Title: 
PLoS genetics

Aging and longevity are complex traits influenced by genetic and environmental factors. To identify quantitative trait loci (QTLs) that control replicative lifespan, we employed an outbred Saccharomyces cerevisiae model, generated by crossing a vineyard and a laboratory strain. The predominant QTL mapped to the rDNA, with the vineyard rDNA conferring a lifespan increase of 41%. The lifespan extension was independent of Sir2 and Fob1, but depended on a polymorphism in the rDNA origin of replication from the vineyard strain that reduced origin activation relative to the laboratory origin.

Author(s): 
Kwan, Elizabeth X.
Foss, Eric J.
Tsuchiyama, Scott
Alvino, Gina M.
Kruglyak, Leonid
Kaeberlein, Matt
Raghuraman, M. K.
Brewer, Bonita J.
Kennedy, Brian K.
Bedalov, Antonio
Publication Title: 
Molecular and Cellular Biology

In the somatic macronucleus of the ciliate Tetrahymena thermophila, the palindromic rRNA gene (rDNA) minichromosome is replicated from an origin near the center of the molecule in the 5' nontranscribed spacer. The replication of this rDNA minichromosome is under both cell cycle and copy number control. We addressed the effect on origin function of transcription through this origin region.

Author(s): 
Pan, W. J.
Gallagher, R. C.
Blackburn, E. H.
Publication Title: 
Molecular and Cellular Biology

In the somatic macronucleus of the ciliate Tetrahymena thermophila, the palindromic rRNA gene (rDNA) minichromosome is replicated from an origin near the center of the molecule in the 5' nontranscribed spacer. The replication of this rDNA minichromosome is under both cell cycle and copy number control. We addressed the effect on origin function of transcription through this origin region.

Author(s): 
Pan, W. J.
Gallagher, R. C.
Blackburn, E. H.
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