Ribosomes

Publication Title: 
PLoS genetics

Cells respond to accumulation of misfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR) signaling pathway. The UPR restores ER homeostasis by degrading misfolded proteins, inhibiting translation, and increasing expression of chaperones that enhance ER protein folding capacity. Although ER stress and protein aggregation have been implicated in aging, the role of UPR signaling in regulating lifespan remains unknown. Here we show that deletion of several UPR target genes significantly increases replicative lifespan in yeast.

Author(s): 
Labunskyy, Vyacheslav M.
Gerashchenko, Maxim V.
Delaney, Joe R.
Kaya, Alaattin
Kennedy, Brian K.
Kaeberlein, Matt
Gladyshev, Vadim N.
Publication Title: 
PloS One

There is increasing evidence that nutrient-sensing machinery is critically involved in the regulation of aging. The insulin/insulin-like growth factor-1 signaling pathway is the best-characterized pathway with an influence on longevity in a variety of organisms, ranging from yeast to rodents. Reduced expression of the receptor for this pathway has been reported to prolong the lifespan; however, the underlying mechanisms are largely unknown. Here we show that haploinsufficiency of Akt1 leads to an increase of the lifespan in mice.

Author(s): 
Nojima, Aika
Yamashita, Masakatsu
Yoshida, Yohko
Shimizu, Ippei
Ichimiya, Harumi
Kamimura, Naomi
Kobayashi, Yoshio
Ohta, Shigeo
Ishii, Naoaki
Minamino, Tohru
Publication Title: 
PLoS genetics

Cells respond to accumulation of misfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR) signaling pathway. The UPR restores ER homeostasis by degrading misfolded proteins, inhibiting translation, and increasing expression of chaperones that enhance ER protein folding capacity. Although ER stress and protein aggregation have been implicated in aging, the role of UPR signaling in regulating lifespan remains unknown. Here we show that deletion of several UPR target genes significantly increases replicative lifespan in yeast.

Author(s): 
Labunskyy, Vyacheslav M.
Gerashchenko, Maxim V.
Delaney, Joe R.
Kaya, Alaattin
Kennedy, Brian K.
Kaeberlein, Matt
Gladyshev, Vadim N.
Publication Title: 
Cancer Research

Human telomerase reverse transcriptase (hTERT; the catalytic protein subunit of telomerase) is subjected to numerous alternative splicing events, but the regulation and function of these splice variants is obscure. Full-length hTERT includes conserved domains that encode reverse transcriptase activity, RNA binding, and other functions. The major splice variant termed ?+?- or ?-deletion is highly expressed in stem and cancer cells, where it codes for a truncated protein lacking most of the reverse transcriptase domain but retaining the known RNA-binding motifs.

Author(s): 
Listerman, Imke
Sun, Jie
Gazzaniga, Francesca S.
Lukas, Jason L.
Blackburn, Elizabeth H.
Publication Title: 
Ultrastructural Pathology

Ultrastructural changes to P. falciparum-infected red blood cells were examined in vitro after treatment with antimalarial drugs. Artesunate had the most rapid parasitocidal effect. All three drugs caused structural changes within the parasite, including dilatation of the parasitophorus vacuole membrane, depletion of ribosomes, mitochondrial swelling, and decreased formation of hemozoin crystals. The structure of surface knobs and Maurer's clefts were similar to controls but reduced in number. Only depletion of free ribosomes correlated with antimalarial drug exposure.

Author(s): 
Sachanonta, Navakanit
Chotivanich, Kesinee
Chaisri, Urai
Turner, Gareth D. H.
Ferguson, David J. P.
Day, Nicholas P. J.
Pongponratn, Emsri
Publication Title: 
Biological & Pharmaceutical Bulletin

Based on phylogenetic analysis of rDNA internal transcribed spacer (ITS) sequences, a pair of specific primers were designed for differentiating the Chinese traditional medicine Hericium species from other mushrooms by PCR. PCR was performed, with total DNAs as a template at an annealing temperature of 52-57 degrees C. Positive amplification was obtained from H. erinaceus with all DNA templates from different resources, but not from other related species. The result indicated that H.

Author(s): 
Lu, Ling
Li, Jieli
Cang, Yihua
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