In Situ Hybridization

Publication Title: 
Schizophrenia Research

In the cerebral prefrontal cortex (PFC), DNA-methyltransferase 1 (DNMT1), the enzyme that catalyzes the methylation of cytosine at carbon atoms in position 5 in CpG dinucleotides, is expressed selectively in GABAergic neurons and is upregulated in layers I and II of schizophrenia (SZ) and bipolar disorder patients with psychosis (BDP).

Veldic, Marin
Kadriu, Bashkim
Maloku, Ekrem
Agis-Balboa, Roberto C.
Guidotti, Alessandro
Davis, John M.
Costa, Erminio
Publication Title: 
The International Journal of Neuropsychopharmacology

BACKGROUND: The human Val66Met polymorphism in brain-derived neurotrophic factor (BDNF), a key factor in neuroplasticity, synaptic function, and cognition, has been implicated in the pathophysiology of neuropsychiatric and neurodegenerative disorders. BDNF is encoded by multiple transcripts with distinct regulation and localization, but the impact of the Val66Met polymorphism on BDNF regulation remains unclear.

Mallei, Alessandra
Baj, Gabriele
Ieraci, Alessandro
Corna, Stefano
Musazzi, Laura
Lee, Francis S.
Tongiorgi, Enrico
Popoli, Maurizio
Publication Title: 
The Journal of Antimicrobial Chemotherapy

Plasmodium berghei ANKA infected C57B1/6 mice develop cerebral malaria at a parasitaemia of 15-25%. When parasitaemia reached 10%, P. berghei infected mice were treated with artemether, chloroquine or clindamycin in order to prevent the occurrence of cerebral malaria. Artemether and chloroquine were highly efficient. Functional tests revealed that zymosan stimulated spleen cells from untreated mice with cerebral malaria showed a slight decrease in their capacity to produce reactive oxygen intermediates (ROI) when compared with naive mice.

Prada, J.
Müller, S.
Bienzle, U.
Kremsner, P. G.
Publication Title: 
Molecular Psychiatry

Corticotropin-releasing hormone (CRH) is a key neuroendocrine factor implementing endocrine, immune and behavioral responses to stress. CRH exerts its action through two major receptors, CRH-R1 and CRH-R2. Recently novel non-peptidic antagonists directed against CRH-R1 or CRH-R2 have been proposed as promising agents in the treatment of depression, anxiety and eating disorder. However, so far the CRH-receptor system has not been widely studied in humans.

Hiroi, N.
Wong, M. L.
Licinio, J.
Park, C.
Young, M.
Gold, P. W.
Chrousos, G. P.
Bornstein, S. R.
Publication Title: 
Human Molecular Genetics

Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by the impairment of cognitive functions and by beta amyloid (Abeta) plaques in the cerebral cortex and the hippocampus. Our objective was to determine genes that are critical for cellular changes in AD progression, with particular emphasis on changes early in disease progression.

Reddy, P. Hemachandra
McWeeney, Shannon
Park, Byung S.
Manczak, Maria
Gutala, Ramana V.
Partovi, Dara
Jung, Youngsin
Yau, Vincent
Searles, Robert
Mori, Motomi
Quinn, Joseph
Publication Title: 
Stem Cells (Dayton, Ohio)

Mesenchymal stem cells (MSCs) exhibit immune-suppressive properties, follow a pattern of multilineage differentiation, and exhibit transdifferentiation potential. Ease in expansion from adult bone marrow, as well as its separation from ethical issues, makes MSCs appealing for clinical application. MSCs treated with retinoic acid resulted in synaptic transmission, based on immunostaining of synaptophysin and electrophysiological studies. In situ hybridization indicated that the neurotransmitter gene preprotachykinin-I was expressed in these cells.

Cho, Kyung Jin
Trzaska, Katarzyna A.
Greco, Steven J.
McArdle, Joseph
Wang, Fu Shun
Ye, Jiang-Hong
Rameshwar, Pranela
Publication Title: 
The American Journal of Pathology

C-reactive protein (CRP) is a risk marker for cardiovascular events in apparently healthy persons. Cogent data show that, aside from the liver, CRP is produced in atherosclerotic lesions, kidney, neurons, and alveolar macrophages. Because several proatherogenic effects of CRP have been documented in endothelial cells, we examined human aortic endothelial cells (HAEC) for CRP production. We detected the presence of CRP mRNA by RT-PCR and in situ hybridization, intracellular protein by Western blot and secreted protein by ELISA.

Venugopal, Senthil Kumar
Devaraj, Sridevi
Jialal, Ishwarlal
Publication Title: 
FASEB journal: official publication of the Federation of American Societies for Experimental Biology

The aim of this research was to validate transcription magnetic resonance (MR) imaging (MRI) for gene transcript targeting in acute neurological disorders in live subjects. We delivered three MR probe variants with superparamagnetic iron oxide nanoparticles (SPION, a T2 susceptibility agent) linked to a phosphorothioate-modified oligodeoxynucleotide (sODN) complementary to c-fos mRNA (SPION-cfos) or beta-actin mRNA (SPION-beta-actin) and to sODN with random sequence (SPION-Ran).

Liu, Christina H.
Huang, Shuning
Cui, Jiankun
Kim, Young R.
Farrar, Christian T.
Moskowitz, Michael A.
Rosen, Bruce R.
Liu, Philip K.
Publication Title: 
Molecular and Cellular Biology

Because heat shock proteins have been shown to play a critical role in protecting cells from hyperthermia and other types of physiological stresses, it was of interest to determine what effect age and caloric restriction have on the ability of cells to regulate the expression of heat shock protein 70 (hsp70), the most prominent and most evolutionarily conserved of the heat shock proteins. Caloric restriction is the only experimental manipulation known to retard aging and increase survival of mammals.

Heydari, A. R.
Wu, B.
Takahashi, R.
Strong, R.
Richardson, A.
Publication Title: 
The American Journal of Physiology

To investigate whether brain leptin involves neuropeptidergic pathways influencing ingestion, metabolism, and gastrointestinal functioning, leptin (3.5 micrograms) was infused daily into the third cerebral ventricular of rats for 3 days. To distinguish between direct leptin effects and those secondary to leptin-induced anorexia, we studied vehicle-infused rats with food available ad libitum and those that were pair-fed to leptin-treated animals.

van Dijk, G.
Seeley, R. J.
Thiele, T. E.
Friedman, M. I.
Ji, H.
Wilkinson, C. W.
Burn, P.
Campfield, L. A.
Tenenbaum, R.
Baskin, D. G.
Woods, S. C.
Schwartz, M. W.


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