Spectrometry, Fluorescence

Publication Title: 
Biochimica Et Biophysica Acta

Botulinum neurotoxins (BoNTs) are proteins of great interest not only because of their extreme toxicity but also paradoxically for their therapeutic applications. All the known serotypes (A-G) have varying degrees of longevity and potency inside the neuronal cell. Differential chemical modifications such as phosphorylation and ubiquitination have been suggested as possible mechanisms for their longevity, but the molecular basis of the longevity remains unclear.

Author(s): 
Kumar, Raj
Kukreja, Roshan V.
Cai, Shuowei
Singh, Bal R.
Publication Title: 
Die Pharmazie

Propofol whole blood and plasma concentrations at offset of hypnosis in eighteen patients were inversely related to patient age and body fat. The relationship between propofol concentrations and body fat is derived from the relationship between age and body fat and age was the single independent predictor of concentrations at offset of propofol hypnosis.

Author(s): 
O'Halloran, P. L.
Hosseini-Yeganeh, M.
McBride, L. J.
Ramzan, I.
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

In this study we have evaluated the application and reliability of using fluorescence (FLUO)-based high throughput screening assays with recombinant CYPs (rCYP). This was accomplished by screening 29 clinically important antiparasitic drugs for inhibition of the five major drug-metabolizing CYPs (-1A2, -2C9, -2C19, -2D6, and -3A4). Data from FLUO/rCYP assays were compared with that obtained by conventional HPLC assays using human liver microsomes (HLM) and rCYPs.

Author(s): 
Bapiro, T. E.
Egnell, A. C.
Hasler, J. A.
Masimirembwa, C. M.
Publication Title: 
Protein Science: A Publication of the Protein Society

Glutathione S-transferase of the malarial parasite Plasmodium falciparum (PfGST) represents a novel class of GST isoenzymes. Since the architecture of the PfGST substrate binding site differs significantly from its human counterparts and there is only this one isoenzyme present in the parasite, PfGST is considered a highly attractive target for antimalarial drug development. Here we report the mechanistic, kinetic, and structural characterization of PfGST as well as its interaction with different ligands.

Author(s): 
Hiller, Nicole
Fritz-Wolf, Karin
Deponte, Marcel
Wende, Wolfgang
Zimmermann, Herbert
Becker, Katja
Publication Title: 
Tumori

AIMS AND BACKGROUND: Previous studies showed that dihydroartemisinin (DHA) possessed antitumor activity in many human tumor cells through the induction of apoptosis. The aim of this study was to investigate the effects of DHA on apoptosis in the human hepatocellular carcinoma cell line HepG2 and the possible molecular mechanisms involved. METHODS: The inhibitory effect of DHA on HepG2 cells was measured by MTT assay. The percentage of apoptotic cells was detected by flow cytometry with double staining of fluorescein isothiocyanate-annexin V/propidium iodide.

Author(s): 
Gao, Xiaoling
Luo, Ziguo
Xiang, Tingxiu
Wang, Kejian
Li, Jian
Wang, Pilong
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis

An Indian patient referred to Clinica del Lavoro 'L.Devoto' of Milano showed clinical signs of heavy metal poisoning, possibly related to a sustained 6-month use of approx. 3 g/day of a traditional preparation (a whitish powder with a 'mineral' appearance) to treat urological problems. To confirm the causal relationship between the disease and the use of such product, metal testing was performed on the patient's hair and the ayurvedic remedy samples by total reflection X-ray fluorescence (TXRF).

Author(s): 
Borgese, L.
Zacco, A.
Bontempi, E.
Pellegatta, M.
Vigna, L.
Patrini, L.
Riboldi, L.
Rubino, F. M.
Depero, L. E.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

The putative oxidation of hydroethidine (HE) has become a widely used fluorescent assay for the detection of superoxide in cultured cells. By covalently joining HE to a hexyl triphenylphosphonium cation (Mito-HE), the HE moiety can be targeted to mitochondria. However, the specificity of HE and Mito-HE for superoxide in vivo is limited by autooxidation as well as by nonsuperoxide-dependent cellular processes that can oxidize HE probes to ethidium (Etd).

Author(s): 
Robinson, Kristine M.
Janes, Michael S.
Pehar, Mariana
Monette, Jeffrey S.
Ross, Meredith F.
Hagen, Tory M.
Murphy, Michael P.
Beckman, Joseph S.
Publication Title: 
Journal of Lipid Research

The metabolism of vitamin E involves oxidation of the phytyl chain to generate the terminal metabolite 7,8-dimethyl-2-(beta-carboxyethyl)-6-hydroxychroman (CEHC) via intermediate formation of 13'-hydroxychromanol and long-chain carboxychromanols. Conjugated (including sulfated) metabolites were reported previously but were limited to CEHCs.

Author(s): 
Jiang, Qing
Freiser, Helene
Wood, Karl V.
Yin, Xinmin
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

Curcuminoid extract and piperine are being evaluated for beneficial effects in Alzheimer's disease, among other intractable disorders. Consequently, we studied the potential for herb-drug interactions involving cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and sulfotransferase (SULT) enzymes. The curcuminoid extract inhibited SULT > CYP2C19 > CYP2B6 > UGT > CYP2C9 > CYP3A activities with IC(50) values ranging from 0.99 +/- 0.04 to 25.3 +/- 1.3 microM, whereas CYP2D6, CYP1A2, and CYP2E1 activities were less affected (IC(50) values > 60 microM).

Author(s): 
Volak, Laurie P.
Ghirmai, Senait
Cashman, John R.
Court, Michael H.
Publication Title: 
Biochemistry

The purpose of the study was to determine the effects of truncation of various regions of betaB1-crystallin on its structural properties and stability of heterooligomers formed by wild-type (WT) betaB1 or its deletion mutants with WT betaA3-crystallin.

Author(s): 
Srivastava, K.
Gupta, R.
Chaves, J. M.
Srivastava, O. P.

Pages

Subscribe to RSS - Spectrometry, Fluorescence