Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Publication Title: 
Methods (San Diego, Calif.)

DNA methylation plays a vital role in normal cellular function, with aberrant methylation signatures being implicated in a growing number of human pathologies and complex human traits. Methods based on the modification of genomic DNA with sodium bisulfite are considered the 'gold-standard' for DNA methylation profiling on genomic DNA; however they require large amounts of DNA and may be prohibitively expensive when used on the large sample sizes necessary to detect small effects.

Author(s): 
Docherty, Sophia J.
Davis, Oliver S. P.
Haworth, Claire M. A.
Plomin, Robert
Mill, Jonathan
Publication Title: 
The Biochemical Journal

Elucidation of the principal targets of the action of the antimalarial drug artemisinin is an ongoing pursuit that is important for understanding the action of this drug and for the development of more potent analogues. We have examined the chemical reaction of Hb with artemisinin. The protein-bound haem in Hb has been found to react with artemisinin much faster than is the case with free haem.

Author(s): 
Kannan, Rangiah
Kumar, Krishan
Sahal, Dinkar
Kukreti, Shrikant
Chauhan, Virander S.
Publication Title: 
Antimicrobial Agents and Chemotherapy

Artemisinins induce drug metabolism through the activation of the pregnane X receptor (PXR) in vitro. Here, we report the resequencing and genotyping of PXR variants in 75 Vietnamese individuals previously characterized for CYP3A enzyme activity after artemisinin exposure. We identified a total of 31 PXR variants, including 5 novel single nucleotide polymorphisms (SNPs), and we identified significantly different allele frequencies relative to other ethnic groups.

Author(s): 
Piedade, Rita
Schaeffeler, Elke
Winter, Stefan
Asimus, Sara
Schwab, Matthias
Ashton, Michael
Burk, Oliver
Gil, José P.
Publication Title: 
PloS One

BACKGROUND: Hemozoin crystals are normally formed in vivo by Plasmodium parasites to detoxify free heme released after hemoglobin digestion during its intraerythrocytic stage. Inhibition of hemozoin formation by various drugs results in free heme concentration toxic for the parasites. As a consequence, in vitro assays have been developed to screen and select candidate antimalarial drugs based on their capacity to inhibit hemozoin formation.

Author(s): 
Thomas, Vincent
Góis, Ana
Ritts, Bruce
Burke, Peter
Hänscheid, Thomas
McDonnell, Gerald
Publication Title: 
Biomedical chromatography: BMC

Nardostachys jatamansi DC. is a highly reputed Medhya and Nootropic (Learning and Memory) Ayurvedic medicinal plant. Its use as herbal medicine singly and as an ingredient of multi-herbal formulations is fast increasing. In order to authenticate and evaluate it quantitatively, its standardization is highly warranted with respect to a reliable marker. In this connection a rapid and highly sensitive UPLC-QTOF MS method has been developed.

Author(s): 
Mallavadhani, Uppuluri Venkata
Panigrahi, Reba
Pattnaik, Banita
Publication Title: 
Journal of Biochemistry and Molecular Biology

The study of whole patterns of changes in protein expression and their modifications, or proteomics, presents both technological advances as well as formidable challenges to biological researchers. Nutrition research and the food sciences in general will be strongly influenced by the new knowledge generated by the proteomics approach. This review examines the different aspects of proteomics technologies, while emphasizing the value of consideration of "traditional" aspects of protein separation.

Author(s): 
Barnes, Stephen
Kim, Helen
Publication Title: 
Molecular & cellular proteomics: MCP

The skin is a highly accessible organ and thus provides an attractive immune environment for cost-effective, simple, and needle-free delivery of vaccines and immunomodulators. In this study, we pretreated mouse skin with an anionic surfactant, sodium lauryl sulfate (SLS), for a short period of time (10 min) followed by epicutaneous vaccination with hen egg lysozyme antigen. We demonstrated for the first time that pretreatment of skin with surfactant SLS significantly enhances the production of antibody to hen egg lysozyme.

Author(s): 
Huang, Chun-Ming
Wang, Chao-Cheng
Kawai, Mikako
Barnes, Stephen
Elmets, Craig A.
Publication Title: 
Journal of Applied Physiology (Bethesda, Md.: 1985)

This paper describes a method of determining protein synthesis and turnover using in vivo labeling of protein with deuterated water and analysis of matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI-TOF) spectrum. Protein synthesis is calculated using mass isotopomer distribution analysis instead of precursor to product amino acid enrichment ratio.

Author(s): 
Xiao, Gary Guishan
Garg, Meena
Lim, Shu
Wong, Derek
Go, Vay Liang
Lee, Wai-Nang Paul
Publication Title: 
Oncology Reports

The chemopreventive efficacy of cranberry juice concentrate in an experimental model of urinary bladder cancer was evaluated using female Fischer-344 rats. The animals received N-butyl-N-(4-hydroxybutyl)-nitrosamine (OH-BBN) for a period of eight weeks. Cranberry juice concentrate was administered at doses of 1.0 or 0.5 ml/rat/day beginning one week after the final OH-BBN treatment and continuing until the end of the study. The urinary bladders of all the rats were weighed and examined grossly for lesions, and all masses were submitted for pathological evaluation.

Author(s): 
Prasain, Jeevan K.
Jones, Kenneth
Moore, Ray
Barnes, Stephen
Leahy, Marge
Roderick, Robin
Juliana, M. Margaret
Grubbs, Clinton J.
Publication Title: 
Analytical Chemistry

Pancreatic cancer MIA PaCa cells were cultured in the presence and absence of (15)N amino acids mixture for 72 h. During protein synthesis, the incorporation of (15)N amino acids results in a new mass isotopomer distribution in protein, which is approximated by the concatenation of two binomial distributions of (13)C and (15)N. The fraction of protein synthesis (FSR) can thus be determined from the relative intensities of the "labeled" (new) and the "unlabeled" (old) spectra.

Author(s): 
Zhao, Yingchun
Lee, Wai-Nang Paul
Lim, Shu
Go, Vay Liang
Xiao, Jing
Cao, Rui
Zhang, Hengwei
Recker, Robert Roy
Xiao, Gary Guishan

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