Tandem Mass Spectrometry

Publication Title: 

Thirty-four polyphenolic substances in methanol extracts of the fruits of Terminalia bellerica, Terminalia chebula and Terminalia horrida, three plants used in Egyptian folk medicine, were initially identified by HPLC-ESI-MS and quantitated by analytical HPLC after column chromatography on Sephadex LH-20. After purification by semi-preparative HPLC the compounds were identified by their mass and fragmentation patterns using ESI-MS-MS. For several compounds detailed 1H/13C NMR analysis at 600 MHz was performed.

Pfundstein, Beate
El Desouky, Samy K.
Hull, William E.
Haubner, Roswitha
Erben, Gerhard
Owen, Robert W.
Publication Title: 
Journal of Food Science

Total phenolic content, total antioxidant activity of Myrobalan plum (Prunus cerasifera Ehrh.) and anthocyanin content in peel were analyzed in this article. In addition, ultra-performance liquid chromatography with photodiode array detection and electrospray ionization tandem mass spectrometry were used to determine anthocyanin composition of this fruit. The range of total phenolic content of the tested samples was 1.34 to 6.11 g/kg fresh weight (FW), and anthocyanin content in fruit peel of Myrobalan plum was from 1.93 to 19.86 g/kg peel.

Wang, Yan
Chen, Xiaoliu
Zhang, Yanmin
Chen, Xuesen
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis

Drug discovery from complex mixture like Chinese herbs is a challenge and extensive false positives make the obtainment of specific bioactive compounds difficult. In the present study, a novel sample preparation method was proposed to rapidly reveal the specific bioactive compounds from complex mixtures using ?-glucosidase as a case. Firstly, aqueous and methanol extracts of 500 traditional Chinese medicines were carried out with the aim of finding new sources of ?-glucosidase inhibitors.

Li, D. Q.
Zhao, J.
Xie, J.
Li, S. P.
Publication Title: 
Molecular Psychiatry

Autism affects males more than females, giving rise to the idea that the influence of steroid hormones on early fetal brain development may be one important early biological risk factor.

Baron-Cohen, S.
Auyeung, B.
N¯rgaard-Pedersen, B.
Hougaard, D. M.
Abdallah, M. W.
Melgaard, L.
Cohen, A. S.
Chakrabarti, B.
Ruta, L.
Lombardo, M. V.
Publication Title: 
Journal of Ethnopharmacology

AIM OF THE STUDY: Around the world, Tilia species have been used in traditional medicine for their properties as tranquilizer. Furthermore, Mexican species of Tilia have been grouped as Tilia americana var. mexicana, but their specific content in flavonoids is poorly described. In this study, inflorescences of Mexican Tilia were collected in three different regions of Mexico to compare their flavonoid content and anxiolytic-like response. MATERIALS AND METHODS: Flavonoid content was analyzed by using an HPLC-MS technique.

Aguirre-Hernández, Eva
González-Trujano, Ma Eva
Martínez, Ana Laura
Moreno, Julia
Kite, Geoffrey
Terrazas, Teresa
Soto-Hernández, Marcos
Publication Title: 
Journal of mass spectrometry: JMS

Neurotransmitters and their metabolites in central nervous system were known to play a significant role in sedation and hypnosis. A rapid and sensitive UFLC-MS/MS method for simultaneous determination of serotonin, 5-hydroxyindole acetic acid (5-HIAA), tryptophan (Try), dopamine (DA), norepinephrine (NE), ?-aminobutyric acid (GABA), glutamic acid (Glu) and acetylcholine (Ach) in rat brain without derivatization, ion-pairing reagent or pre-concentration was developed.

He, Bosai
Bi, Kaishun
Jia, Ying
Wang, Jiahong
Lv, Chunxiao
Liu, Ran
Zhao, Longshan
Xu, Huarong
Chen, XiaoHui
Li, Qing
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

Five metabolites of the antimalarial piperaquine (PQ) (1,3-bis-[4-(7-chloroquinolyl-4)-piperazinyl-1]-propane) have been identified and their molecular structures characterized. After a p.o. dose of dihydroartemisinin-piperaquine, urine collected over 16 h from two healthy subjects was analyzed using liquid chromatography (LC)/UV, LC/tandem mass spectrometry (MS/MS), Fourier transform ion cyclotron resonance (FTICR)/MS, and H NMR.

Tarning, J.
Bergqvist, Y.
Day, N. P.
Bergquist, J.
Arvidsson, B.
White, N. J.
Ashton, M.
Lindegardh, N.
Publication Title: 
Antimicrobial Agents and Chemotherapy

The two fixed-dose combinations of dihydroartemisinin and piperaquine (Artekin and Arterakine) were found to be bioinequivalent in healthy Vietnamese subjects. However, because the peak plasma concentrations and areas under the concentration-time curves of dihydroartemisinin and piperaquine were only marginally different between the two formulations, similar therapeutic efficacies are expected in the treatment of malaria infections.

Chinh, Nguyen Trong
Quang, Nguyen Ngoc
Thanh, Nguyen Xuan
Dai, Bui
Geue, Jason P.
Addison, Russell S.
Travers, Thomas
Edstein, Michael D.
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis

A liquid chromatographic tandem mass spectroscopy method for the quantification of artemisinin in human heparinised plasma has been developed and validated. The method uses Oasis HLB mu-elution solid phase extraction 96-well plates to facilitate a high throughput of 192 samples a day. Artesunate (internal standard) in a plasma-water solution was added to plasma (50 microL) before solid phase extraction.

Lindegardh, N.
Tarning, J.
Toi, P. V.
Hien, T. T.
Farrar, J.
Singhasivanon, P.
White, N. J.
Ashton, M.
Day, N. P. J.
Publication Title: 
Malaria Journal

BACKGROUND: Dihydroartemisinin (DHA), a powerful anti-malarial drug, has been used as monotherapy and artemisinin-based combination therapy (ACT) for more than decades. So far, however, the tissue distribution and metabolic profile of DHA data are not available from animal and humans. METHODS: Pharmacokinetics, tissue distribution, mass balance, and elimination of [14C] DHA have been studieded in rats following a single intravenous administration. Protein binding was performed with rat and human plasma.

Xie, Lisa H.
Li, Qigui
Zhang, Jing
Weina, Peter J.


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