Tissue Culture Techniques

Publication Title: 
British Journal of Anaesthesia

BACKGROUND: Inhaled anaesthetics (IAs) produce multiple dose-dependent behavioural effects including amnesia, hypnosis, and immobility in response to painful stimuli that are mediated by distinct anatomical, cellular, and molecular mechanisms. Amnesia is produced at lower anaesthetic concentrations compared with hypnosis or immobility. Nicotinic acetylcholine receptors (nAChRs) modulate hippocampal neural network correlates of memory and are highly sensitive to IAs.

Author(s): 
Westphalen, R. I.
Gomez, R. S.
Hemmings, H. C.
Publication Title: 
Anesthesia and Analgesia

BACKGROUND: Benzodiazepines are widely used in clinical anesthesia as premedication, but also to induce general anesthesia. Recent in vitro studies suggest that ?-aminobutyric acid type A receptors, harboring a classical high-affinity benzodiazepine binding site, possess another "nonclassical" binding site for benzodiazepines. At present, it is unclear if, and to what extent, this novel nonclassical binding site is of relevance for the actions of benzodiazepines in the central nervous system.

Author(s): 
Drexler, Berthold
Zinser, Stefan
Hentschke, Harald
Antkowiak, Bernd
Publication Title: 
Journal of Translational Medicine

Ayurveda is one of the ancient systems of health care of Indian origin. Roughly translated into "Knowledge of life", it is based on the use of natural herbs and herb products for therapeutic measures to boost physical, mental, social and spiritual harmony and improve quality of life. Although sheltered with long history and high trust, ayurveda principles have not entered laboratories and only a handful of studies have identified pure components and molecular pathways for its life-enhancing effects.

Author(s): 
Deocaris, Custer C.
Widodo, Nashi
Wadhwa, Renu
Kaul, Sunil C.
Publication Title: 
PloS One

Diabetes mellitus is recognized as a leading cause of new cases of blindness. The prevalence of diabetic eye disease is expected to continue to increase worldwide as a result of the dramatic increase in the number of people with diabetes. At present, there is no medical treatment to delay or prevent the onset and progression of cataract or retinopathy, the most common causes of vision loss in diabetics. The plant Emblica officinalis (gooseberry) has been used for thousands of years as a traditional Indian Ayurvedic preparation for the treatment of diabetes in humans.

Author(s): 
Puppala, Muthenna
Ponder, Jessica
Suryanarayana, Palla
Reddy, Geereddy Bhanuprakash
Petrash, J. Mark
LaBarbera, Daniel V.
Publication Title: 
Journal of Translational Medicine

Ayurveda is one of the ancient systems of health care of Indian origin. Roughly translated into "Knowledge of life", it is based on the use of natural herbs and herb products for therapeutic measures to boost physical, mental, social and spiritual harmony and improve quality of life. Although sheltered with long history and high trust, ayurveda principles have not entered laboratories and only a handful of studies have identified pure components and molecular pathways for its life-enhancing effects.

Author(s): 
Deocaris, Custer C.
Widodo, Nashi
Wadhwa, Renu
Kaul, Sunil C.
Publication Title: 
Phytotherapy research: PTR

Curcuma longa (turmeric) has been used widely as a spice, particularly in Asian countries. It is also used in the Ayurvedic system of medicine as an antiinflammatory and antimicrobial agent and for numerous other curative properties. The aim of this study was to investigate the effects of an aqueous extract of Curcuma longa (AEC) on tissues involved in glucose homeostasis. The extract was prepared by soaking 100 g of ground turmeric in 1 L of water, which was filtered and stored at -20°C prior to use.

Author(s): 
Mohankumar, Sureshkumar
McFarlane, James R.
Publication Title: 
PloS One

Diabetes mellitus is recognized as a leading cause of new cases of blindness. The prevalence of diabetic eye disease is expected to continue to increase worldwide as a result of the dramatic increase in the number of people with diabetes. At present, there is no medical treatment to delay or prevent the onset and progression of cataract or retinopathy, the most common causes of vision loss in diabetics. The plant Emblica officinalis (gooseberry) has been used for thousands of years as a traditional Indian Ayurvedic preparation for the treatment of diabetes in humans.

Author(s): 
Puppala, Muthenna
Ponder, Jessica
Suryanarayana, Palla
Reddy, Geereddy Bhanuprakash
Petrash, J. Mark
LaBarbera, Daniel V.
Publication Title: 
Journal of Vascular Surgery

BACKGROUND: Defensins are cysteine-rich cationic polypeptides released from neutrophils that exhibit powerful antimicrobial activities. Because inflammation, including neutrophil infiltration and release of defensins, may play an important role in atherosclerosis and other vascular diseases, we determined whether alpha-defensin could cause endothelial dysfunction, a major initial event of atherosclerosis, in porcine coronary arteries. METHODS: Porcine coronary arteries were sliced into 5-mm rings and treated with different concentrations of human recombinant alpha-defensin for 24 hours.

Author(s): 
Kougias, Panagiotis
Chai, Hong
Lin, Peter H.
Yao, Qizhi
Lumsden, Alan B.
Chen, Changyi
Publication Title: 
Journal of Alternative and Complementary Medicine (New York, N.Y.)

BACKGROUND: Although acupuncture-needle manipulation is an important component of acupuncture therapy, little information is currently available on whether or not specific types of needle manipulation produce different effects on the body. Bidirectional (back-and-forth) rotation is one of the most common forms of needle manipulation used in acupuncture practice.

Author(s): 
Langevin, Helene M.
Bouffard, Nicole A.
Churchill, David L.
Badger, Gary J.
Publication Title: 
Clinical Immunology (Orlando, Fla.)

Oral bromelain has been anecdotally reported to decrease inflammation in ulcerative colitis (UC). Proteolytically active bromelain is known to decrease expression of mRNAs encoding pro-inflammatory cytokines by human leukocytes in vitro. To assess the effect of bromelain on mucosal secretion of cytokines in inflammatory bowel disease (IBD), endoscopic colon biopsies from patients with UC, Crohn's disease (CD), and non-IBD controls were treated in vitro with bromelain or media, then cultured. Secretion of pro-inflammatory cytokines and chemokines was measured.

Author(s): 
Onken, Jane E.
Greer, Paula K.
Calingaert, Brian
Hale, Laura P.

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