Transferrin

Publication Title: 
Anticancer Research

BACKGROUND: Artemisinin is a chemical compound extracted from the wormwood plant, Artemisia annua L. It has been shown to selectively kill cancer cells in vitro and retard the growth of implanted fibrosarcoma tumors in rats. In the present research, we investigated its mechanism of cytotoxicity to cancer cells. MATERIALS AND METHODS: Molt-4 cells, in complete RPMI-1640 medium, were first incubated with 12 microM of human holotransferrin at 37 degrees C in a humid atmosphere of 5% CO2 for one hour. This enhanced the iron supply to the cells.

Author(s): 
Singh, Narendra P.
Lai, Henry C.
Publication Title: 
Anticancer Research

BACKGROUND: Artemisinin is a compound isolated from the wormwood Artemisia annua L. It reacts with iron and forms cytotoxic free radicals. It is selectively more toxic to cancer than normal cells because cancer cells contain significantly more intracellular free iron. Previously, we found that covalently tagging artemisinin to transferrin enhanced the selectivity and toxicity of artemisinin toward cancer cells in vitro. In the present research, artemisinin-transferrin conjugate was tested in a rat breast cancer model.

Author(s): 
Lai, Henry
Nakase, Ikuhiko
Lacoste, Eric
Singh, Narendra P.
Sasaki, Tomikazu
Publication Title: 
PloS One

Heme (Fe2+ protoporphyrin IX) is an essential molecule that has been implicated the potent antimalarial action of artemisinin and its derivatives, although the source and nature of the heme remain controversial. Artemisinins also exhibit selective cytotoxicity against cancer cells in vitro and in vivo. We demonstrate that intracellular heme is the physiologically relevant mediator of the cytotoxic effects of artemisinins.

Author(s): 
Zhang, Shiming
Gerhard, Glenn S.
Publication Title: 
Asian Pacific journal of cancer prevention: APJCP

Cytotoxic activity of artemisinin and derivatives in the presence and absence of holo-transferrin and expression of genes involved in resistance of cancer cells were investigated in human cholangiocarcinoma (CL-6) and hepatocarcinoma (Hep-G2) cell lines in vitro. After incubation with the test drugs and 5-fluorouracil (5-FU) cytotoxicity was asessed by MTT assay.

Author(s): 
Chaijaroenkul, Wanna
Viyanant, Vithoon
Mahavorasirikul, Wiratchanee
Na-Bangchang, Kesara
Publication Title: 
PloS One

Artemisinin and its main active metabolite dihydroartemisinin, clinically used antimalarial agents with low host toxicity, have recently shown potent anticancer activities in a variety of human cancer models. Although iron mediated oxidative damage is involved, the mechanisms underlying these activities remain unclear. In the current study, we found that dihydroartemisinin caused cellular iron depletion in time- and concentration-dependent manners. It decreased iron uptake and disturbed iron homeostasis in cancer cells, which were independent of oxidative damage.

Author(s): 
Ba, Qian
Zhou, Naiyuan
Duan, Juan
Chen, Tao
Hao, Miao
Yang, Xinying
Li, Junyang
Yin, Jun
Chu, Ruiai
Wang, Hui
Publication Title: 
Daru: Journal of Faculty of Pharmacy, Tehran University of Medical Sciences

BACKGROUND: Artemisinin is the major sesquiterpene lactones in sweet wormwood (Artemisia annua L.), and its combination with transferrin exhibits versatile anti-cancer activities. Their non-selective targeting for cancer cells, however, limits their application. The aim of this study was to prepare the artemisinin and transferrin-loaded magnetic nanoliposomes in thermosensitive and non-thermosensitive forms and evaluate their antiproliferative activity against MCF-7 and MDA-MB-231 cells for better tumor-targeted therapy.

Author(s): 
Gharib, Amir
Faezizadeh, Zohreh
Mesbah-Namin, Seyed Ali Reza
Saravani, Ramin
Publication Title: 
Clinical journal of the American Society of Nephrology: CJASN

BACKGROUND: Because of the risk of performing renal biopsies in children with co-morbid conditions, we carried out this study to identify candidate protein biomarkers in the urine of HIV-infected children with renal disease. DESIGN, SETTING, PARTICIPANTS & MEASUREMENTS: Urine samples from HIV-infected children with biopsy proven HIV-nephropathy (HIVAN; n = 4), HIV-associated Hemolytic Uremic Syndrome (HIV-HUS; n = 2), or no renal disease (n = 3) were analyzed by two-dimensional electrophoresis (2-DE) and proteomic methods.

Author(s): 
Soler-García, Angel A.
Johnson, Douglas
Hathout, Yetrib
Ray, Patricio E.
Publication Title: 
American Journal of Physiology. Gastrointestinal and Liver Physiology

Dietary iron is particularly critical during periods of rapid growth such as in neonatal development. Human and rodent studies have indicated that iron deficiency or excess during this critical stage of development can have significant long- and short-term consequences. Since the requirement for iron changes during development, the availability of adequate iron is critical for the differentiation and maturation of individual organs participating in iron homeostasis.

Author(s): 
Hegde, Narasimha V.
Unger, Erica L.
Jensen, Gordon L.
Hankey, Pamela A.
Paulson, Robert F.
Publication Title: 
International Journal of Nanomedicine

Emodin is a multifunctional Chinese traditional medicine with poor water solubility. D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) is a pegylated vitamin E derivate. In this study, a novel liposomal-emodin-conjugating TPGS was formulated and compared with methoxypolyethyleneglycol 2000-derivatized distearoyl-phosphatidylethanolamine (mPEG2000-DSPE) liposomal emodin. TPGS improved the encapsulation efficiency and stability of emodin egg phosphatidylcholine/cholesterol liposomes.

Author(s): 
Wang, Tiechuang
Yin, Xiaodong
Lu, Yaping
Shan, Weiguang
Xiong, Subin
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