Withanolides

Publication Title: 
Chemical & Pharmaceutical Bulletin

Multiple shoot cultures of two experimental lines of Withania somnifera plants (RS-Selection-1 and RS-Selection-2) were established using nodal segments as explants. The hormonal combinations of benzyl adenine and kinetin not only influenced their morphogenetic response but also differentially modulated the level of biogeneration of withanolide A in the in vitro shoots of the two lines. Interestingly, withanolide-A, that was hardly detectable in the aerial parts of field-grown Withania somnifera (explant source), accumulated considerably in the in vitro shoot cultures of the plant.

Author(s): 
Sangwan, Rajender Singh
Chaurasiya, Narayan Das
Lal, Payare
Misra, Laxminarain
Uniyal, Girish Chandra
Tuli, Rakesh
Sangwan, Neelam Singh
Publication Title: 
Journal of Translational Medicine

Ayurveda is one of the ancient systems of health care of Indian origin. Roughly translated into "Knowledge of life", it is based on the use of natural herbs and herb products for therapeutic measures to boost physical, mental, social and spiritual harmony and improve quality of life. Although sheltered with long history and high trust, ayurveda principles have not entered laboratories and only a handful of studies have identified pure components and molecular pathways for its life-enhancing effects.

Author(s): 
Deocaris, Custer C.
Widodo, Nashi
Wadhwa, Renu
Kaul, Sunil C.
Publication Title: 
Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan

Ashwagandha (root of Withania somnifera) has been used for many purposes, it is mainly considered a tonic in traditional Ayurvedic medicine. This review focuses on the effects of compounds isolated from Ashwagandha on dementia models and on the spinal cord injury model. Our study demonstrated that the active constituents, withanolide A, withanoside IV, and withanoside VI, restored presynapses and postsynapses, in addition to both axons and dendrites in cortical neurons after Abeta(25-35)-induced injury.

Author(s): 
Tohda, Chihiro
Publication Title: 
Cancer Research

Withaferin A (WA) is derived from the medicinal plant Withania somnifera, which has been safely used for centuries in Indian Ayurvedic medicine for treatment of different ailments. We now show, for the first time, that WA exhibits significant activity against human breast cancer cells in culture and in vivo. The WA treatment decreased viability of MCF-7 (estrogen-responsive) and MDA-MB-231 (estrogen-independent) human breast cancer cells in a concentration-dependent manner.

Author(s): 
Stan, Silvia D.
Hahm, Eun-Ryeong
Warin, Renaud
Singh, Shivendra V.
Publication Title: 
Nutrition and Cancer

Withaferin A (WA) is derived from the medicinal plant Withania somnifera that has been safely used for centuries in the Indian Ayurvedic medicine for treatment of various ailments. We now demonstrate that WA treatment causes G2 and mitotic arrest in human breast cancer cells.

Author(s): 
Stan, Silvia D.
Zeng, Yan
Singh, Shivendra V.
Publication Title: 
Carcinogenesis

We have shown previously that withaferin A (WA), a promising anticancer constituent of Ayurvedic medicine plant Withania somnifera, inhibits growth of human breast cancer cells in culture and in vivo in association with apoptosis induction. The present study builds on these observations and demonstrates that WA inhibits constitutive as well as interleukin-6 (IL-6)-inducible activation of signal transducer and activator of transcription 3 (STAT3), which is an oncogenic transcription factor activated in many human malignancies including breast cancer.

Author(s): 
Lee, Joomin
Hahm, Eun-Ryeong
Singh, Shivendra V.
Publication Title: 
BMC genomics

BACKGROUND: Nuclear Factor kappa B (NF-κB) is a transcription factor involved in the regulation of cell signaling responses and is a key regulator of cellular processes involved in the immune response, differentiation, cell proliferation, and apoptosis. The constitutive activation of NF-κB contributes to multiple cellular outcomes and pathophysiological conditions such as rheumatoid arthritis, asthma, inflammatory bowel disease, AIDS and cancer. Thus there lies a huge therapeutic potential beneath inhibition of NF-κB signalling pathway for reducing these chronic ailments.

Author(s): 
Grover, Abhinav
Shandilya, Ashutosh
Punetha, Ankita
Bisaria, Virendra S.
Sundar, Durai
Publication Title: 
Molecular Carcinogenesis

We have shown previously that withaferin A (WA), a promising anticancer constituent of Ayurvedic medicine plant Withania somnifera, inhibits growth of MCF-7 and MDA-MB-231 human breast cancer cells in culture and MDA-MB-231 xenografts in vivo by causing apoptosis. However, the mechanism of WA-induced apoptosis is not fully understood. The present study was designed to systematically determine the role of tumor suppressor p53 and estrogen receptor-α (ER-α) in proapoptotic response to WA using MCF-7, T47D, and ER-α overexpressing MDA-MB-231 cells as a model.

Author(s): 
Hahm, Eun-Ryeong
Lee, Joomin
Huang, Yi
Singh, Shivendra V.
Publication Title: 
PloS One

Withaferin A (WA), a promising anticancer constituent of Ayurvedic medicinal plant Withania somnifera, inhibits growth of MDA-MB-231 and MCF-7 human breast cancer cells in culture and MDA-MB-231 xenografts in vivo in association with apoptosis induction, but the mechanism of cell death is not fully understood. We now demonstrate, for the first time, that WA-induced apoptosis is mediated by reactive oxygen species (ROS) production due to inhibition of mitochondrial respiration.

Author(s): 
Hahm, Eun-Ryeong
Moura, Michelle B.
Kelley, Eric E.
Van Houten, Bennett
Shiva, Sruti
Singh, Shivendra V.
Publication Title: 
PloS One

The medicinal plant Withania somnifera has been used for over centuries in Indian Ayurvedic Medicine to treat a wide spectrum of disorders. Withaferin A (WA), a bioactive compound that is isolated from this plant, has anti-inflammatory, immuno-modulatory, anti-angiogenic, and anti-cancer properties. Here we investigated malignant pleural mesothelioma (MPM) suppressive effects of WA and the molecular mechanisms involved.

Author(s): 
Yang, Huanjie
Wang, Ying
Cheryan, Vino T.
Wu, Wenjuan
Cui, Cindy Qiuzhi
Polin, Lisa A.
Pass, Harvey I.
Dou, Q. Ping
Rishi, Arun K.
Wali, Anil

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