Publication Title: 
The Biochemical Journal

NaCT (sodium-coupled citrate transporter) is an Na(+)-coupled citrate transporter identified recently in mammals that mediates the cellular uptake of citrate. It is expressed predominantly in the liver. NaCT is structurally and functionally related to the product of the Indy (I'm not dead yet) gene in Drosophila, the dysfunction of which leads to lifespan extension. Here, we show that NaCT mediates the utilization of extracellular citrate for fat synthesis in human liver cells, and that the process is stimulated by lithium.

Inoue, Katsuhisa
Zhuang, Lina
Maddox, Dennis M.
Smith, Sylvia B.
Ganapathy, Vadivel
Publication Title: 
PLoS genetics

We have taken an engineering approach to extending the lifespan of Caenorhabditis elegans. Aging stands out as a complex trait, because events that occur in old animals are not under strong natural selection. As a result, lifespan can be lengthened rationally using bioengineering to modulate gene expression or to add exogenous components. Here, we engineered longer lifespan by expressing genes from zebrafish encoding molecular functions not normally present in worms. Additionally, we extended lifespan by increasing the activity of four endogenous worm aging pathways.

Sagi, Dror
Kim, Stuart K.
Publication Title: 
Cardiovascular Toxicology

In 2011, dozens of children and pregnant women in Korea died by exposure to sterilizer for household humidifier, such as Oxy(Æ) and Cefu(Æ). Until now, however, it remains unknown how the sterilizer affect the human health to cause the acute deaths. To find its toxicity for organ, we investigated the putative toxicity of the sterilizer in the cardiovascular system. The sterilizers, polyhexamethylene guanidine phosphate (PHMG, Cefu(Æ)), and oligo-[2-(2-ethoxy)-ethoxyethyl)-guanidinium-chloride (PGH, Oxy(Æ)) were treated to human lipoproteins, macrophages, and dermal fibroblast cells.

Kim, Jae-Yong
Kim, Hak Hyeon
Cho, Kyung-Hyun
Publication Title: 
Disease Models & Mechanisms

The study of telomere biology is crucial to the understanding of aging and cancer. In the pursuit of greater knowledge in the field of human telomere biology, the mouse has been used extensively as a model. However, there are fundamental differences between mouse and human cells. Therefore, additional models are required. In light of this, we have characterized telomerase-deficient zebrafish (Danio rerio) as the second vertebrate model for human telomerase-driven diseases.

Anchelin, Monique
Alcaraz-PÈrez, Francisca
MartÌnez, Carlos M.
BernabÈ-GarcÌa, Manuel
Mulero, Victoriano
Cayuela, MarÌa L.
Publication Title: 
Molecular Cell

Histone H3K4 demethylase LSD1 plays an important role in stem cell biology, especially in the maintenance of the silencing of differentiation genes. However, how the function of LSD1 is regulated and the differentiation genes are derepressed are not understood. Here, we report that elimination of LSD1 promotes embryonic stem cell (ESC) differentiation toward neural lineage. We showed that the destabilization of LSD1 occurs posttranscriptionally via the ubiquitin-proteasome pathway by an E3 ubiquitin ligase, Jade-2.

Han, Xiao
Gui, Bin
Xiong, Cong
Zhao, Linnan
Liang, Jing
Sun, Luyang
Yang, Xiaohan
Yu, Wenhua
Si, Wenzhe
Yan, Ruorong
Yi, Xia
Zhang, Di
Li, Wanjin
Li, Lifang
Yang, Jianguo
Wang, Yan
Sun, Yi Eve
Zhang, Dai
Meng, Anming
Shang, Yongfeng
Publication Title: 
Journal of Cellular and Molecular Medicine

Artemisinins are plant products with a wide range of medicinal applications. Most prominently, artesunate is a well tolerated and effective drug for treating malaria, but is also active against several protozoal and schistosomal infections, and additionally exhibits anti-angiogenic, anti-tumorigenic and anti-viral properties. The array of activities of the artemisinins, and the recent emergence of malaria resistance to artesunate, prompted us to synthesize and evaluate several novel artemisinin-like derivatives.

Soomro, Shahid
Langenberg, Tobias
Mahringer, Anne
Konkimalla, V. Badireenath
Horwedel, Cindy
Holenya, Pavlo
Brand, Almut
Cetin, Canan
Fricker, Gert
Dewerchin, Mieke
Carmeliet, Peter
Conway, Edward M.
Jansen, Herwig
Efferth, Thomas
Publication Title: 
Acta Pharmacologica Sinica

AIM: To investigate the embryotoxicity of dihydroartemisinin (DHA), the main active metabolite of artemisinin, in zebrafish, and explore the corresponding mechanisms. METHODS: The embryos of wild type and TG (flk1:GFP) transgenic zebrafish were exposed to DHA. Developmental phenotypes of the embryos were observed. Development of blood vessels was directly observed in living embryos of TG (flk1:GFP) transgenic zebrafish under fluorescence microscope.

Ba, Qian
Duan, Juan
Tian, Jia-qiang
Wang, Zi-liang
Chen, Tao
Li, Xiao-guang
Chen, Pei-zhan
Wu, Song-jie
Xiang, Li
Li, Jing-quan
Chu, Rui-ai
Wang, Hui
Publication Title: 
Journal of Molecular Endocrinology

The zebrafish model system is one of the most widely used animal models for developmental research and it is now becoming an attractive model for drug discovery and toxicological screening. The completion of sequencing the zebrafish genome and the availability of full-length cDNAs and DNA microarrays for expression analysis, in addition to techniques for generating transgenic lines and targeted mutations, have made the zebrafish model even more attractive to researchers.

Elo, B.
Villano, C. M.
Govorko, D.
White, L. A.
Publication Title: 
Journal of Cosmetic Dermatology

BACKGROUND: Cell migration, angiogenesis, inflammation, and extracellular matrix remodeling are key events in wound healing. Natural products, including fatty acids (FAs), can accelerate wound healing by modulating the aforementioned events. AIMS: This study aims to evaluate the effect of lucuma (Pouteria lucuma O Kezte) nut oil (LNO) on fibroblasts migration, angiogenesis, inflammation, bacterial and fungal growth, and wound healing. Methods  GC-MS analysis of FAs methyl esters (FAMES) was used for chemical characterization of LNO.

Rojo, Leonel E.
Villano, Caren M.
Joseph, Gili
Schmidt, Barbara
Shulaev, Vladimir
Shuman, Joel L.
Lila, Mary Ann
Raskin, Ilya
Publication Title: 
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-Chief) ... [et Al.]

At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating this task. Performing multiple sample extractions, concentrating sample extracts, and using several separation and detection methods are common strategies to overcome these challenges but require a great amount of resources.

Kirkwood, Jay S.
Maier, Claudia
Stevens, Jan F.


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