Cytoplasm

Publication Title: 
Experimental Cell Research

Normal human diploid cells, TIG-1, ceased to proliferate at about the 62 population doubling level (PDL). Transformed clones isolated from TIG-1 cells infected with wtSV40 and those with tsA900 SV40 cultured at 34 degrees C were subcultured up to about 80 PDL. When the culture temperature of tsA SV40-transformed cells was shifted from 34 to 39.5 degrees C at 51 PDL, the growth curve of these transformed cells changed to that of normal young cells.

Author(s): 
Ide, T.
Tsuji, Y.
Nakashima, T.
Ishibashi, S.
Publication Title: 
International Journal of Hematology

FKHRL1 is one of the human homologues of DAF-16, which is concerned with longevity in Caenorhabditis elegans. Previously, we demonstrated that FKHRL1 functions downstream of Akt in erythropoietin (EPO) signaling and that it is directly phosphorylated by activated Akt. Because phosphorylated FKHRL1 loses its transcriptional activity and translocates into the cytoplasm, FKHRL1 appears to be nonfunctional in the presence of EPO.

Author(s): 
Uchida, Mie
Kirito, Keita
Endo, Hitoshi
Ozawa, Keiya
Komatsu, Norio
Publication Title: 
Clinics in Endocrinology and Metabolism
Author(s): 
Merry, B. J.
Phillips, J. G.
Publication Title: 
Cryobiology
Author(s): 
Blackburn, E. D.
King, J. E.
Pace, W. G.
Thomford, N. R.
Publication Title: 
Molecular Cancer Therapeutics

The present study was undertaken to gain insights into the molecular mechanism of cell death (apoptosis) by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, using PC-3 human prostate cancer cells as a model. The viability of PC-3 cells, but not a normal prostate epithelial cell line (PrEC), was reduced significantly on treatment with guggulsterone in a concentration-dependent manner.

Author(s): 
Singh, Shivendra V.
Zeng, Yan
Xiao, Dong
Vogel, Victor G.
Nelson, Joel B.
Dhir, Rajiv
Tripathi, Yamini B.
Publication Title: 
Molecular Cancer Therapeutics

The present study was undertaken to gain insights into the molecular mechanism of cell death (apoptosis) by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, using PC-3 human prostate cancer cells as a model. The viability of PC-3 cells, but not a normal prostate epithelial cell line (PrEC), was reduced significantly on treatment with guggulsterone in a concentration-dependent manner.

Author(s): 
Singh, Shivendra V.
Zeng, Yan
Xiao, Dong
Vogel, Victor G.
Nelson, Joel B.
Dhir, Rajiv
Tripathi, Yamini B.
Publication Title: 
Phytomedicine: International Journal of Phytotherapy and Phytopharmacology

Bacopa monnieri (BM) an herb, found throughout the Indian subcontinent in wet, damp and marshy areas is used in Ayurvedic system of medicine for improving intellect/memory, treatment of anxiety and neuropharmacological disorders. Although extensively given to children as a memory enhancer, no data exists on its ability to modulate neuronal oxidative stress in prepubertal animal models.

Author(s): 
Shinomol, George K.
Muralidhara, null
Publication Title: 
The Journal of Nutrition

Despite recent advances in antibiotic therapy and intensive care, sepsis remains a widespread problem in critically ill patients. The high mortality from sepsis is in part mediated by bacterial endotoxin, which stimulates macrophages/monocytes to sequentially release early (e.g., tumor necrosis factor, interleukin-1, and interferon-gamma) and late [e.g., high mobility group box 1 protein (HMGB1)] proinflammatory cytokines. Our discovery of HMGB1 as a late mediator of lethal systemic inflammation has initiated a new field of investigation for the development of experimental therapeutics.

Author(s): 
Wang, Haichao
Li, Wei
Li, Jianhua
Rendon-Mitchell, Beatriz
Ochani, Mahendar
Ashok, Mala
Yang, Lihong
Yang, Huan
Tracey, Kevin J.
Wang, Ping
Sama, Andrew E.
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

In response to inflammatory stimuli (e.g., endotoxin, proinflammatory cytokines) or oxidative stress, macrophages actively release a ubiquitous nuclear protein, high-mobility group box 1 (HMGB1), to sustain an inflammatory response to infection or injury. In this study, we demonstrated mild heat shock (e.g., 42.5 degrees C, 1 h), or enhanced expression of heat shock protein (Hsp) 72 (by gene transfection) similarly rendered macrophages resistant to oxidative stress-induced HMGB1 cytoplasmic translocation and release.

Author(s): 
Tang, Daolin
Kang, Rui
Xiao, Weimin
Jiang, Lei
Liu, Meidong
Shi, Yongzhong
Wang, Kangkai
Wang, Haichao
Xiao, Xianzhong
Publication Title: 
Molecular Biology of the Cell

Maintenance of intestinal mucosal epithelial integrity requires polyamines that modulate the expression of various genes involved in cell proliferation and apoptosis. Recently, polyamines were shown to regulate the subcellular localization of the RNA-binding protein HuR, which stabilizes its target transcripts such as nucleophosmin and p53 mRNAs. The activating transcription factor-2 (ATF-2) mRNA encodes a member of the ATF/CRE-binding protein family of transcription factors and was computationally predicted to be a target of HuR.

Author(s): 
Xiao, Lan
Rao, Jaladanki N.
Zou, Tongtong
Liu, Lan
Marasa, Bernard S.
Chen, Jie
Turner, Douglas J.
Zhou, Huiping
Gorospe, Myriam
Wang, Jian-Ying

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