Culture Media

Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Previously we reported [Packer, L. & Smith, J.R. (1974) Proc. Natl. Acad. Sci. USA 71, 4763-4767] that the lifespan of WI-38 human diploid fibroblasts in vitro was significantly increased by continuously growing the cell cultures in the presence of vitamin E(dl-alpha-tocopherol), but in 19 subsequent subcultivation series we were unable to reproduce these findings. While vitamin E is incorporated into the cells and is able to act effectively as an antioxidant, apparantly is intracellular antioxidant properties alone do not routinely result in an increase of cell lifespan.

Author(s): 
Packer, L.
Smith, J. R.
Publication Title: 
Journal of Cellular Physiology

Various concentrations of oxygen were used to determine the optimum culture medium PO2 for survival and proliferation of attached human and mouse fibroblasts grown from different inoculum sizes. When T-15 flasks were seeded with less than or equal to 2 X 10(4) cells (less than or equal to 1.3 X 10(3) cells/cm2), the highest plating efficiencies and cell yields were obtained with a culture medium PO2 of 40-60 mm Hg.

Author(s): 
Taylor, W. G.
Camalier, R. F.
Sanford, K. K.
Publication Title: 
Experimental Cell Research

Numerous studies have shown that supplementation of the growth medium of human fibroblasts with dexamethasone at physiologic concentrations extends replicative lifespan up to 30%. While this extension of lifespan has been used to probe various aspects of the senescent phenotype, no mechanism for the increased lifespan of human fibroblasts grown in the presence of dexamethasone has ever been identified.

Author(s): 
Mawal-Dewan, Madhu
Frisoni, Lorenza
Cristofalo, Vincent J.
Sell, Christian
Publication Title: 
Journal of Endodontics

INTRODUCTION: Dental pulp stem cells (DPSCs) have received much attention as a promising population of stem cells in regenerative endodontics. Securing a good blood supply during regeneration is a challenging task because of the constricted apical canal opening, which allows only a limited blood supply. The aim of this study was to investigate any potential synergistic effects of dental pulp stem cells and endothelial cells (ECs) on osteo-/odontogenic and angiogenic differentiation in vitro.

Author(s): 
Dissanayaka, Waruna Lakmal
Zhan, Xuan
Zhang, Chengfei
Hargreaves, Kenneth M.
Jin, Lijian
Tong, Edith H. Y.
Publication Title: 
Cell Proliferation

OBJECTIVES: This study has intended to investigate longevity of subcutaneous fat-derived mesenchymal stem cells (SF-MSCs) under extensive culturing. It has also focused on optimization of culture media for them over prolonged periods in vitro. MATERIALS AND METHODS: We evaluated SF-MSCs with reference to phenotypic characterization, proliferative ability, karyotype stability and differentiation potency with early (P3) and late passage (P20) conditions, using four different media, DMEM-LG, ALPHA-MEM, DMEM-F12 and DMEM-KO.

Author(s): 
Dhanasekaran, M.
Indumathi, S.
Rashmi, M.
Rajkumar, J. S.
Sudarsanam, D.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Mutations in the clk-1 gene of the nematode Caenorhabditis elegans result in slowed development, sluggish adult behaviors, and an increased lifespan. CLK-1 is a mitochondrial polypeptide with sequence and functional conservation from human to yeast. Coq7p, the Saccharomyces cerevisiae homologue, is essential for ubiquinone (coenzyme Q or Q) synthesis and therefore respiration. However, based on assays of respiratory function, it has been reported that the primary defect in the C. elegans clk-1 mutants is not in Q biosynthesis.

Author(s): 
Jonassen, T.
Larsen, P. L.
Clarke, C. F.
Publication Title: 
Science (New York, N.Y.)
Author(s): 
Strauss, Evelyn
Publication Title: 
Fungal genetics and biology: FG & B

The Candida albicans Goa1p is required for mitochondrial functions. In a strain lacking GOA1 (GOA31), respiration, mitochondrial membrane potential, complex I (CI) activity of the electron transport chain, and ATP synthesis are significantly decreased. A shortened chronological life span (CLS) of GOA31 occurs in 2% glucose that is associated with an increase in cell reactive oxidant species (ROS) and apoptosis. We now show that caloric restriction (CR) in media containing 0.5% glucose instead of 2% glucose-SC extends the CLS to the level of parental and gene-reconstituted strains.

Author(s): 
Chen, Hui
Calderone, Richard
Sun, Nuo
Wang, Yun
Li, Dongmei
Publication Title: 
Experimental Gerontology

We have previously shown that autophagy is required for chronological longevity in the budding yeast Saccharomyces cerevisiae. Here we examine the requirements for autophagy during extension of chronological life span (CLS) by calorie restriction (CR). We find that autophagy is upregulated by two CR interventions that extend CLS: water wash CR and low glucose CR. Autophagy is required for full extension of CLS during water wash CR under all growth conditions tested.

Author(s): 
Aris, John P.
Alvers, Ashley L.
Ferraiuolo, Roy A.
Fishwick, Laura K.
Hanvivatpong, Amanda
Hu, Doreen
Kirlew, Christine
Leonard, Michael T.
Losin, Kyle J.
Marraffini, Michelle
Seo, Arnold Y.
Swanberg, Veronica
Westcott, Jennifer L.
Wood, Michael S.
Leeuwenburgh, Christiaan
Dunn, William A.
Publication Title: 
PloS One

The traditional view on dietary restriction has been challenged with regard to extending lifespan of the fruit fly Drosophila melanogaster. This is because studies have shown that changing the balance of dietary components without reduction of dietary intake can increase lifespan, suggesting that nutrient composition other than dietary restriction play a pivotal role in regulation of longevity. However, this opinion has not been reflected in yeast aging studies.

Author(s): 
Wu, Ziyun
Liu, Shao Quan
Huang, Dejian

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