Transfection

Publication Title: 
The Journal of Neuroscience: The Official Journal of the Society for Neuroscience

Ketamine has important anesthetic, analgesic, and psychotropic actions. It is widely believed that NMDA receptor inhibition accounts for ketamine actions, but there remains a dearth of behavioral evidence to support this hypothesis. Here, we present an alternative, behaviorally relevant molecular substrate for anesthetic effects of ketamine: the HCN1 pacemaker channels that underlie a neuronal hyperpolarization-activated cationic current (I(h)).

Author(s): 
Chen, Xiangdong
Shu, Shaofang
Bayliss, Douglas A.
Publication Title: 
Science (New York, N.Y.)

Plasmodium falciparum chloroquine resistance is a major cause of worldwide increases in malaria mortality and morbidity. Recent laboratory and clinical studies have associated chloroquine resistance with point mutations in the gene pfcrt. However, direct proof of a causal relationship has remained elusive and most models have posited a multigenic basis of resistance.

Author(s): 
Sidhu, Amar Bir Singh
Verdier-Pinard, Dominik
Fidock, David A.
Publication Title: 
In Vivo (Athens, Greece)

The anti-malarial artesunate (ART) also inhibits the growth of cancer cells. The active moiety is an endoperoxide bridge whose cleavage generates reactive oxygen species and free radicals. We analyzed whether glutathione-related enzymes contribute to tumor resistance to ART and to the low toxicity of ART towards normal organs.

Author(s): 
Efferth, Thomas
Volm, Manfred
Publication Title: 
Molecular Cancer Therapeutics

The sesquiterpene lactone dihydroartemisinin (DHA), a semisynthetic derivative of the herbal antimalaria drug artemisinin, is cytotoxic to human tumor cells. Treatment of Jurkat T-lymphoma cells with DHA induced a breakdown of the mitochondrial transmembrane potential, release of cytochrome c, activation of caspases, and DNA fragmentation indicative of apoptosis induction.

Author(s): 
Handrick, René
Ontikatze, Teona
Bauer, Kerstin-Daniela
Freier, Florian
Rübel, Amelie
Dürig, Jan
Belka, Claus
Jendrossek, Verena
Publication Title: 
Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University

OBJECTIVE: To construct recombinant expression vectors of small interfering RNA (siRNA) targeting survivin and investigate apoptosis of glioma cell line U251 mediated by the survivin-targeting siRNA. METHODS: According to the sequence of the coding region of survivin gene, two strings of 19 nucleotides of inverted sequence flanking the loop sequence of two complementary 9-base oligonucleotides were designed and synthesized to form hairpin construct as the DNA templates for the target siRNA.

Author(s): 
Xu, Ru-xiang
Tu, Yan-yang
Jiang, Xiao-dan
Feng, Jiang-nan
Huang, Jun
Publication Title: 
The Journal of Pharmacology and Experimental Therapeutics

An extract of the plant Coleus forskohlii has been used for centuries in Ayurvedic medicine to treat various diseases such as hypothyroidism, heart disease, and respiratory disorders. Additionally, complex herbal mixtures containing this extract are gaining popularity in United States for their putative "fat-burning" properties. The active ingredient in C. forskohlii extract is the diterpene compound forskolin.

Author(s): 
Ding, Xunshan
Staudinger, Jeff L.
Publication Title: 
The Journal of Pharmacology and Experimental Therapeutics

Guggulsterone is the active ingredient in gugulipid, an organic extract of the Commiphora mukul plant. Gugulipid has been used for nearly 3000 years in Ayurvedic medicine, mainly as a treatment for arthritis. Herbal practitioners currently use gugulipid therapy in conditions as diverse as rheumatism, coronary artery disease, arthritis, hyperlipidemia, acne, and obesity. The active ingredient in gugulipid is guggulsterone, a plant sterol compound recently identified as a pregnane X receptor (PXR; NR1I2) ligand.

Author(s): 
Ding, Xunshan
Staudinger, Jeff L.
Publication Title: 
Cancer Research

Withaferin A (WA) is derived from the medicinal plant Withania somnifera, which has been safely used for centuries in Indian Ayurvedic medicine for treatment of different ailments. We now show, for the first time, that WA exhibits significant activity against human breast cancer cells in culture and in vivo. The WA treatment decreased viability of MCF-7 (estrogen-responsive) and MDA-MB-231 (estrogen-independent) human breast cancer cells in a concentration-dependent manner.

Author(s): 
Stan, Silvia D.
Hahm, Eun-Ryeong
Warin, Renaud
Singh, Shivendra V.
Publication Title: 
The Journal of Pharmacology and Experimental Therapeutics

An extract of the plant Coleus forskohlii has been used for centuries in Ayurvedic medicine to treat various diseases such as hypothyroidism, heart disease, and respiratory disorders. Additionally, complex herbal mixtures containing this extract are gaining popularity in United States for their putative "fat-burning" properties. The active ingredient in C. forskohlii extract is the diterpene compound forskolin.

Author(s): 
Ding, Xunshan
Staudinger, Jeff L.
Publication Title: 
The Journal of Pharmacology and Experimental Therapeutics

Guggulsterone is the active ingredient in gugulipid, an organic extract of the Commiphora mukul plant. Gugulipid has been used for nearly 3000 years in Ayurvedic medicine, mainly as a treatment for arthritis. Herbal practitioners currently use gugulipid therapy in conditions as diverse as rheumatism, coronary artery disease, arthritis, hyperlipidemia, acne, and obesity. The active ingredient in gugulipid is guggulsterone, a plant sterol compound recently identified as a pregnane X receptor (PXR; NR1I2) ligand.

Author(s): 
Ding, Xunshan
Staudinger, Jeff L.

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